Transcript MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde Univ. of Arkansas John Clark WSU Cameron Peace Dorrie Main Kate Evans Univ.

MSU
Amy Iezzoni (PD)
Jim Hancock
Dechun Wang
Cholani Weebadde
Univ. of Arkansas
John Clark
WSU
Cameron Peace
Dorrie Main
Kate Evans
Univ. of Minnesota
Karina Gallardo
Jim Luby
Vicki McCracken
Chengyan Yue
Nnadozie Oraguzie
Former WSU
Oregon State Univ.
Raymond Jussaume
Alexandra Stone
Mykel Taylor
Cornell
Susan Brown
Kenong Xu
Clemson
Ksenija Gasic
Gregory Reighard
Texas A&M
Dave Byrne
USDA-ARS
Nahla Bassil
Gennaro Fazio Univ. of CA-Davis
Chad Finn
Tom Gradziel
Carlos Crisosto
Plant Research Intl,
Netherlands
Univ. of New Hamp.
Eric van de Weg
Tom Davis
Marco Bink
Acknowledgements
This project is supported by the Specialty
Crop Research Initiative of USDA’s
National Institute of Food and Agriculture
International Collaborators
Plant Research International, Netherlands
East Malling Research, UK
Andres Bello University, Chile
CRA-FRU, Rome, Italy
Plant & Food Research, New Zealand
IASMA, Trento, Italy
ARC, South Africa
INRA (Bordeaux, Avignon, Angers), France
RosBREED
participant
location
+ 2 Scientific Advisory Panel members (Spain, New Zealand)
Discovering a QTL
A major milestone or a stepping-stone into
practical marker-assisted breeding?
Cholani Weebadde
25 July 2013
ASHS, Palm Desert, CA
Outline of Presentation
 How does QTL discovery inform MAB?
 The RosBREED MAB pipeline - a strategy to
put DNA knowledge into practical application
 Workshop presentations
How does QTL discovery inform
Marker-Assisted Breeding
(MAB)?
What is Marker-Assisted Breeding?
Use of DNA information to support breeding decisions
Which wild germplasm to use?
Which traits to target? Which
parents & combinations to create?
Which seedlings to
progress?
Which selections
to trial?
Which advanced selections
to commercialize?
What published QTLs look like
 A bump on the
chromosome
 What does it tell the
breeder??? Nothing
much really! Just that
there may be a gene
in the “bump region”
of the chromosome
that is associated
with the trait
Known QTLs and their application
 Hundreds of
published
QTLs are
currently
available for
Rosaceae
crops
 Of these, only
a few were
being used
for MAB
RosBREED has bridged this chasm
RosBREED is dedicated to putting DNA knowledge into practical
application to increase the efficiency of rosaceous crop breeding
Genomics
Resources
100s of QTLs
published
Markerassisted
breeding
More efficient
development of new
cultivars
RosBREED Mission Statement
We will develop and apply marker-assisted breeding,
based on improved knowledge of industry value and
consumer preferences, to accelerate and increase
the efficiency of rosaceous cultivar release and
successful cultivar adoption
Amy Iezzoni, Michigan State Univ.
Cameron Peace, Wash. State Univ.
4 yrs
$7.2M federal
$7.2M matching
This project is supported
by the Specialty Crop
Research Initiative of
USDA’s National Institute of
Food and Agriculture
Demonstration
Breeders
&
Breeding
Trainees
How we bridged the Chasm….
The RosBREED MAB Pipeline:
a strategy to put DNA knowledge into practical
application….
Jewels in the genome analogy
An individual’s genome is the full complement of genetic
information that it inherited from its parents.
Within this vast repertoire of genetic information, individual’s
genes are being discovered that control critical production
and fruit quality traits.
Jewels
= valuable genetic discoveries ready for breeding application
= marker-locus-trait associations validated in a breeding program
Find
Polish
Put into application
in breeding
So What?!!
www.rocktumbler.com
Lots of Shiny Rocks!
Each breeder needs to
appraise for themselves
all these “gemstones”…
…to decide which ones
are worth polishing…
…to find the
“jewels”!
Socio-Economics Surveys (example for apple)
Breeders
Washington
Producers
Fruit flavor
Fruit crispness
15
Exterior color
26
Fruit firmness
6
7 5
Shelf life at retail
7
7 3
Sweetness/soluble solids
6
7 3
Sugar/acid balance
9
7
External appearance
13
No storage disorders
7 4
Disease resistance 2 5
Storage life 5
Other fruit quality attributes 2 3
Size 3
Juiciness 2
Tartness
Shape
Phytonutrient
Aroma
% of respondents
Michigan
Producers
43
Market
Intermediaries
41
23
23
10
So that resources for development of genetic
tests (polishing the shiny rocks) can be directed
to traits with the highest importance to
0
20
40
60
80
100
stakeholders….
RosBREED MAB Pipeline
• Integrate DNA information with conventional breeding,
systematically (8-stage MAB Pipeline)
“Jewels in the Genome” Available Now!
Information on the jewels on eXtension
“RosBREED Jewels are now featured in eXtension”
Workshop presentations
RosBREED success stories:
QTL and QTL allele validation in Cherry, Apple and Peach
Known
QTLs
1. Amy Iezzoni – Cherry
Breeding
markers
Simple
validation
2. Sujeet Verma – Apple
3. Ksenija Gasic - Peach
Allele effects
& distributions
Application
in breeding
Marker-Assisted Breeding for Apple:
Experiences from University of Minnesota and
Washington State University
Known
QTLs
Breeding
markers
Simple
validation
Allele effects
& distributions
Jim Luby
RosBREED Breeding Team Lead
Application
in breeding