Transcript Blood Start Quit TABLE OF CONTENTS Blood Components Blood Typing Blood Cell Differential Identification Blood Cell Counting Using a Hemocytometer Quit Blood BLOOD TYPING ABO Agglutination Result Rh Agglutination Result Type A Type AB Rh.

Blood
Start
Quit
TABLE OF CONTENTS
Blood Components
Blood Typing
Blood Cell Differential Identification
Blood Cell Counting Using a Hemocytometer
Quit
Blood
BLOOD TYPING
ABO Agglutination Result
Rh Agglutination Result
Type A
Type AB
Rh +
Type B
Type O
Rh -
Antigen – Antibody Composition
Blood Type Compatibility Comparisons
Quit
Blood
ABO Blood Typing
Type A
Type B
Type AB
Type O
Quit
Blood
Type A Agglutination Result
Agglutination
Note the appearance
of fine granulation
(pepper grains) in the blood
No
Agglutination
Note the lack of granulation
in the blood
Quit
Blood
Type B Agglutination Result
No
Agglutination
Agglutination
Quit
Blood
Type AB Agglutination Result
Agglutination
Agglutination
Quit
Blood
Type O Agglutination Result
No
Agglutination
No
Agglutination
Quit
Blood
Rh Blood Typing
Rh+
Rh-
Quit
Blood
Rh+ Agglutination Result
Agglutination
Quit
Blood
Rh- Agglutination Result
No
Agglutination
Quit
Blood
BLOOD CELL DIFFERENTIAL
IDENTIFICATION
Human Blood Smear
Erythrocytes
Leukocytes
Thrombocytes
Quit
400x
400x
1000x
Cell Types
400x
1000x
Blood
Human Blood Smear 400x
erythrocyte
thrombocyte
leukocyte
Quit
Blood
Erythrocytes 400x
Quit
Blood
Erythrocytes 1000x
Quit
Blood
LEUKOCYTES
Neutrophil
1000x
Eosinophil
1000x
Basophil
1000x
Lymphocyte
1000x
Monocyte
1000x
Quit
Blood
Leukocyte - Neutrophil 1000x
thrombocyte
erythrocyte
neutrophil
Quit
Blood
Leukocyte - Eosinophil 1000x
erythrocyte
eosinophil
thrombocyte
Quit
Blood
Leukocyte - Basophil 1000x
erythrocyte
basophil
thrombocyte
Quit
Blood
Leukocyte - Lymphocyte 1000x
thrombocyte
lymphocyte
erythrocyte
Quit
Blood
Leukocyte - Monocyte 1000x
erythrocyte
monocyte
thrombocyte
Quit
Blood
Thrombocytes 400x
thrombocytes
Quit
Blood
Thrombocytes 1000x
thrombocytes
Quit
Blood
BLOOD CELL COUNTING USING
A HEMOCYTOMETER
Microvolume Pipetters
Hemocytometer
Loading the Hemocytometer
Hemocytometer Grid (Unloaded) 40x
Hemocytometer Grid (Unloaded) 100x
Hemocytometer Grid (Unloaded) 400x
Hemocytometer Grid (Loaded) 100x
Hemocytometer Grid (Loaded) 400x
Quit
Blood
Microvolume Pipetters
Quit
Blood
Hemocytometer
grid
grid
Quit
Blood
Loading Hemocytometer
pipet tip
cover slip
Quit
Blood
Hemocytometer Grid (Unloaded) 40x
1
2
3
4
5
RBC counted in
5 numbered areas
Quit
Blood
Hemocytometer Grid (Unloaded) 100x
3
Area 3 of previous
magnification
Quit
Blood
Hemocytometer Grid (Unloaded) 400x
Quit
Blood
Hemocytometer Grid (Loaded) 100x
Hemocytometer
loaded with RBC
Quit
Blood
Hemocytometer Grid (Loaded) 400x
cell not
counted
inside of each box,
cell not counted if
on bottom line or
on right line
cell
counted
inside of each box,
cell counted if within
box, on top line or on
left line
Quit
Blood
separate from the
lighter, liquid
components (plasma)
When whole blood is
spun down in a
centrifuge, the heavier,
solid component
(cells) …
Quit
Blood
click on a blood component
Quit
Blood
PLASMA (~55%) includes
water, proteins,
electrolytes, antibodies,
organic nutrients and
organic wastes
Quit
Blood
ERYTHROCYTES
(~45%)
Quit
Blood
“BUFFY COAT” (~1%)
LEUKOCYTES and
THROMBOCYTES
Quit
Blood
ANTIGEN-ANTIBODY COMPOSITION
TYPE A
Rh+
TYPE B
Rh-
TYPE AB
TYPE O
Quit
Blood
Antigen
Antibody
Type A Whole Blood
Antigen
Antibody
b
A
RBC
Quit
Blood
Antigen
Antibody
Type B Whole Blood
Antigen
Antibody
B
a
RBC
Quit
Blood
Antigens
Antigen
Type AB Whole Blood
Antibodies
A
Antigen
B
RBC
Quit
Blood
Antigens
Type O Whole Blood
Antibodies
Antigen
Antibody
B
a
Antibody
b
RBC
Quit
Blood
Antigen
Antibody
Rh+ Whole Blood
Antigen
Antibody
Rh+
Antigen
B
a
RBC
Quit
Blood
Antigen
Antibody
Rh- Whole Blood
Antigen
Antibody
B
a
RBC
Quit
Blood
BLOOD TYPE COMPATIBILITY COMPARISONS
Type A RBC
Type B RBC
Type A plasma
Type AB plasma
Type A plasma
Type AB plasma
Type B plasma
Type O plasma
Type B plasma
Type O plasma
Type AB RBC
Type O RBC
Type A plasma
Type AB plasma
Type A plasma
Type AB plasma
Type B plasma
Type O plasma
Type B plasma
Type O plasma
Quit
Blood
Type A RBC
crossed with
Type A Plasma
Antigen
Antibody
A
b
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type A RBC
crossed with
Antigen
Type B Plasma
Antibody
Antibody
aa
A
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type A RBC
crossed with
Type AB Plasma
Antigen
A
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type A RBC
crossed with
Antigen
Type O Plasma
Antibody
Antibody
aa
A
Antibody
b
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type B RBC
crossed with
Type B Plasma
Antigen
Antibody
Antibody
B
aa
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type B RBC
crossed with
Antigen
Type A Plasma
Antibody
Antibody
B
ba
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type B RBC
crossed with
Type AB Plasma
Antigen
Antibody
B
a
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type B RBC
crossed with
Type O Plasma
Antibody
Antibody
Antigen
aa
B
Antibody
b
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type AB RBC
crossed with
Antigen
Antigen
Type A Plasma
Antibody
A
A
b
Antigen
Antigen
B
B
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type AB RBC
crossed with
Antigen
Antigen
Type B Plasma
Antibody
a
A
A
Antigen
Antigen
B
B
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type AB RBC
crossed with
Type AB Plasma
Antigen
Antigen
A
A
Antigen
Antigen
B
B
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type AB RBC
crossed with
Antigen
Antigen
Type O Plasma
Antibody
a
A
A
Antibody
Antigen
Antigen
b
B
B
RBC
Agglutination YES
Hemolysis YES
Compatible NO
Quit
Blood
Type O RBC
crossed with
Type A Plasma
Antigen
Antibody
Antibody
B
ba
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type O RBC
crossed with
Type B Plasma
Antigen
Antibody
Antibody
B
aa
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type O RBC
crossed with
Type AB Plasma
Antigen
Antibody
B
a
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES
Type O RBC
crossed with
Type O Plasma
Antibody
Antibody
Antigen
aa
B
Antibody
b
RBC
Agglutination
Hemolysis
Compatible
Quit
Blood
NO
NO
YES