Transcript SPIFE IFE and QS

Rev 4/28/06
SPIFE 3000
• Semi-automated
electrophoresis system
• 11 analytes, with multiple
gel sizes for every workload
• Typical throughput
– up to 14 SPE
and 12 IFE gels
in 8 hours
• Used in hundreds
of medical centers
and reference laboratories
worldwide
SPIFE 3000
• Automated sample
application system
• Automated reagent
application for enzymatic
assays
• Temperature-controlled
electrophoresis, incubation
and drying
SPIFE 3000
• Automated staining
and drying of visible
analytes
• Positive ID of sample
tray and gel
SPIFE Assays
• Cholesterol Profile
• Serum Proteins
• Split-Beta SPE
• Urine Proteins
• Immunofixation
• Urine Immunofixation
• Hi Resolution Proteins
• Alkaline Hemoglobins
• Acid Hemoglobins
• CK & LD Isoenzymes
• Alk Phosphatase Isoenzymes
• CSF IgG IEF
• Lipoproteins
SPIFE IFE
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1, 2, 3, 6, 9 and 15 profile gels to
handle a wide range of needs
Up to 210 profiles in 8 hours
Antisera affinity & avidity
balanced for highest sensitivity
with least background
• Acid violet stain enhances
visualization
• Built-in QC wells
SPIFE IFE Procedure
• Load patients samples and
applicator blades
• Dilute automatically using
the Auto Sample Handler
or manually
• Set gel and electrodes in
place in electrophoresis
chamber
SPIFE IFE Procedure
• Blot surface of the gel and
begin electrophoresis
• SPIFE applies samples,
allows for absorption, and
begins electrophoresis
• After electrophoresis,
remove buffer ridges
from the gel
SPIFE IFE Procedure
• Fast and efficient antisera
application
• Set Rigid Antisera
Template in place
• Pipette color-coded
antisera for an entire
profile at once
SPIFE IFE Procedure
• Absorb excess antisera
from channels, preventing
cross-contamination
• Blot gel surface, using
antisera template as press
and allow SPIFE to
incubate and dry the gel
SPIFE IFE Procedure
• Move gel to SPIFE’s
staining unit for
automatic wash, stain,
destain and dry
• Finished!
All SPIFE IFE gels include
integral control wells for
optional use of ImmunoFix
Controls to verify antisera
reactivity
New Educational CD
Protein Electrophoresis and IFE
An Interpretive Guide
• 108 slides
• Basics of protein structure
• Disease states associated
with protein abnormalities
• Suggested protocol for
evaluating monoclonals
• When to run, how to run,
and what to look for
• Patterns to interpret from
simple to complex
• Covers serum and urine
Auto Sample Handler
•
Provides positive
sample ID from tube
to tray to gel
•
Primary tube
sampling with
liquid level
sensing
•
Bar code
reader with
LIS interface
Auto Sample Handler
• Automatically performs
dilutions for IFE
• Prepares sample trays,
ready for SPIFE
• Interfaces to
QuickScan 2000 for
workload listing
QuickScan 2000
• Provide IFE and
SPE results on a
single report
• Patterns can be
viewed and edited
over the LAN
• 10,000 gel archive
with easy retrieval
QuickScan 2000
• Scan anything large or
small in seconds:
10-sample to 100-sample
gels and L/S plates
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Simple to use –
no slits or
wavelengths to
select
Customized interpretive analysis
and decision limits for fast reporting
QuickScan 2000
• Multi-pattern display colorcoded for in/out of range
and reviewed results
• Build your own
decision limits and
interpretive
comments library
• Comments
automatically added
for “normals” and
coded for quick
retrieval
QuickScan 2000
• Choose quantitation of
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split beta SPE as one or
two fractions
Report formats can be
totally customized
Profile up to 100 scans
SPIFE System
Contact your local Helena technical representative for
more information or to arrange an in-lab
demonstration of the SPIFE System.
1-800-231-5663