Transcript プロテオーム解析 微生研 小林 大哲 V.vC 培養後期 7.5hr V.vC 培養

プロテオーム解析
微生研
小林
大哲
V.vC 培養後期 7.5hr
485
234
223
484
302
219
455
476
344
1797
447
482
440
569
913
568
1790
491
734
1791
922
1201
1192
1072
1085
780
1071
993
1788
1293
1061
1454
1487
1792
1441
1795
1566
1568
1796
1789
V.vC 培養後期 7.5hr
解析結果
LA N K
4
1
1
1
1
1
S po t.N O . P ro te in .N O .
2007/4/3
1293
V V 1 _2 2 2 4
1487
440
V V 1 _0 1 5 9
455
V V 1 _0 1 5 9
491
V V 2 _0 5 6 9
568
V V 1 _2 2 6 6
569
V V 1 _2 2 6 6
734
V V 2 _0 8 5 4
P ro te in n am e
B L - - ppm
P e ptide to l. ±
S c o re
A B C - ty p e u n c h a ra c te riz e d tra n s p o rt s y s te m , p e rip la s m ic c o m p o n e n t
87
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
P h o s p h o m a n n o m u ta s e
F u m a ra te h y d ra ta s e
F u m a ra te h y d ra ta s e
T ry p to p h a n a s e
53
78
110
70
70
176
2007/4/4
1
1
1
1
1
1192
1201
1566
1568
1796
219
223
780
V V 2720
V V 1 _0 1 5 8
V V 1 _0 1 5 8
o u te r m e m b ra n e p ro te in O m p U
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
20
V V 1 _1 3 3 8
V V 2 _0 1 8 8
e lo n g a tio n fa c to r E F - 2
s e rin e h y d ro x y m e th y ltra n s fe ra s e
30
50
92
92
62
0
0
74
69
解析結果
LA N K
4
1
1
1
1
1
S po t.N O . P ro te in .N O .
2007/4/3
1293
V V 1 _2 2 2 4
1487
440
V V 1 _0 1 5 9
455
V V 1 _0 1 5 9
491
V V 2 _0 5 6 9
568
V V 1 _2 2 6 6
569
V V 1 _2 2 6 6
734
V V 2 _0 8 5 4
P ro te in n am e
B L - - ppm
P e ptide to l. ±
S c o re
A B C - ty p e u n c h a ra c te riz e d tra n s p o rt s y s te m , p e rip la s m ic c o m p o n e n t
87
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
P h o s p h o m a n n o m u ta s e
F u m a ra te h y d ra ta s e
F u m a ra te h y d ra ta s e
T ry p to p h a n a s e
53
78
110
70
70
176
2007/4/4
1
1
1
1
1
1192
1201
1566
1568
1796
219
223
780
V V 2720
V V 1 _0 1 5 8
V V 1 _0 1 5 8
o u te r m e m b ra n e p ro te in O m p U
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
s u c c in a te d e h y d ro g e n a s e c a ta ly tic s u b u n it
20
V V 1 _1 3 3 8
V V 2 _0 1 8 8
e lo n g a tio n fa c to r E F - 2
s e rin e h y d ro x y m e th y ltra n s fe ra s e
30
50
92
92
62
0
0
74
69
比較
Voyager Spec #1=>BC[BP = 1267.7, 7057]
1267.7546
100
7056.7
90
80
Spot No.491 / Phosphomannomutase
% Intensity
70
60
50
1666.0284
40
1176.8042
1137.6417
30
1536.9415
2212.2369
20
10
1196.7695
1020.6741
1045.7233
1475.9166
1185.8519 1323.8158
0
999.0
2226.2453
1650.9434
1717.0036
1707.9364
1399.4
2128.3221
1799.8
2385.0789
2200.2
2600.6
0
3001.0
Mass (m/z)
Voyager Spec #1=>BC[BP = 1175.4, 3896]
100
1175.3654
3896.5
90
2211.5597
80
1045.4096
Spot No.1487
% Intensity
70
60
50
40
30
20
2225.5639
1309.3187
1179.3963
1493.4373
1320.3410 1475.4696
1305.4033
1434.4841
1165.3833
1537.4640
1357.4555
1107.3701
2103.5963
1716.4676
1707.4020
1792.3260
1994.5106
2839.5369
2823.5522
2239.5727 2384.3560
2298.5864
10
0
999.0
1399.4
1799.8
2200.2
Mass (m/z)
2600.6
0
3001.0
今後の方針


同定不可能な原因を突き止める
残り４スポットを同定する