Transcript Project Wise 2004 - Monroe Community College

Project Wise 2004
Biotechnology:
Performing the Technique of Gel
Electrophoresis
James Rules!
James is a biological all-star! 
What is Electrophoresis?
• The separation of biological
macromolecules in an
electrical field.
• Separation is based on:
– Charge
– Shape
– Size
• Electrophoresis has many,
many uses
– DNA Fingerprinting,
Sequencing.
– It is used for catching the bad
guy/gal
• C.S.I.
I wonder
if they will
find out I
killed the
lab tech.
Bill Ryan
is next!
Lab 1: EDVOTEK #102:
Restriction Enzyme Cleavage of
DNA
• In this lab we used restriction enzymes in
order to cut DNA at specific areas.
Lab 2: EDVOTEK #104:
Size Determination of DNA Restriction
Fragments
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Lab 3: EDVOTEK #105
Mapping of Restriction Sites on Plasmid DNA
• The purpose of this lab is to accurately
locate the genes on the chromosome.
• This is used in the Human Genome
Project.
Have a Great End to Your
Summer!
Project Wise Bio-Tech Group:
Laurie Asermily
Sheri Backus
Joe Bonanno
Davette Carpenter
Amber Corbin
Elaine Esan
Shawn Flanagan
Vonda McLeon
Jane O’Donnell
Steve Polo
Beth Shea