Transcript GK-12 Saturday Workshop - UCI

GK-12 Saturday Workshop
RS: Roxanna Ochoa
CT: Charie Poderoso
October 20, 2012
In this lab, students will be introduced to
restriction enzymes, DNA profiling and gel
electrophoresis
Standards Addressed
• Cell Biology 1a, 1c
• Genetics 2d, 2e
• Investigation & Experimentation 7a, 7c
Timeline
Day 1
• Introduce DNA
fingerprinting
• RE and electrophoresis
activity
Day 2
• DNA fingerprinting
Worksheet
• DNA fingerprinting video
• Students will observe gel
and determine guilty
suspect
DNA
• DNA makes you unique. Your DNA is different from
every other person in the world.
• An individual’s DNA forms a unique pattern of bands
that can be used to identify the person.
• This unique banding pattern produced by fragments
of your DNA is called DNA fingerprinting.
• Law enforcement is able to gather the smallest
amount of evidence at a crime scene and compare it
to DNA from a suspect’s blood or saliva.
Day 1: Restriction Enzymes and
electrophoresis
• Introduce restriction enzymes and
electrophoresis (PPT presentation)
• Activity (Restriction Enzymes and electrophoresis Worksheet)
Restriction Enzymes and
electrophoresis
Day 2: Crime Scene
• Introduce students to crime scene scenario
• Class demonstration
• Load gels with suspect and crime scene DNA
(FisherScientific #S97676)
• Simplify lab by using dye samples (kit or make own)
• During gel electrophoresis:DNA fingerprinting
video.
• Analyze data
Lab Worksheet
Alternative
• NOVA Fingerprinting
High School Modification
• Students can make their own gels and prepare
their own DNA
• Explain electrophoresis and restriction
enzymes in more detail
• Change crime scene scenario