Transcript The Effect of Cold Storage on the Survival of Coccinella

The Effect of Cold Storage on the
Survival of
Coccinella undecimpunctata
Cary Langley
Biology Department
Eastern CT State University
Possible storage of Coccinella
undecimpunctata (Col.,
Coccinellidae) under low temperature
and its effect on some biological
characteristics
A. H. Abdel-Salam and N. F. AbdelBaky
The Journal of Applied Entomology
Vol. 124: 169-176
Coccinella undecimpunctata
•Common Name = Eleven Spotted Lady Beetle
•Predators of

Aphids
Whiteflies
•Biological Control Agents
•Cold Stored for Shipping Purposes
Weevils
Why Study Cold Storage of
Coccinella undecimpunctata
• Survival of Cold Temperatures
• Developmental Stages Surviving the
Cold
• Developmental Stage Best Used for
Short and Long-Term Shipping
Cold Storage Effect
Stages Used
•
•
•
•
Egg
First, Second, Third & Fourth Larval Instars
Pupae
Adults: Directly After Eclosion Without Food
Five Days After Eclosion With Food
Ten Days After Eclosion With Food
• Refrigerated At 6°C
• Refrigerated For 7, 15, 30, 45, 60 Days
Results of the Study
Eggs Hatched (%)
Hatching Success of Refrigerated Eggs
70
60
50
40
30
20
10
0
7
15
30
45
Storage Periods (Days)
60
Survival of Larval Instars
(%)
Larval Instars Success Rate
100
1st Larval
Instar
80
60
2nd Larval
Instar
40
3rd Larval
Instar
20
4th Larval
Instar
0
7
15
30
45
60
Storage Periods (Days)
Survival of Larval Instars
(%)
Larval Instars Success Rate
100
1st Larval
Instar
80
60
2nd Larval
Instar
40
3rd Larval
Instar
20
4th Larval
Instar
0
7
15
30
45
60
Storage Periods (Days)
Survival of Larval Instars
(%)
Larval Instars Success Rate
100
1st Larval
Instar
80
60
2nd Larval
Instar
40
3rd Larval
Instar
20
4th Larval
Instar
0
7
15
30
45
60
Storage Periods (Days)
Larval Instars Success Rate
100
90
1st
Larval
Instar
Survival of Larval Instars
(%)
80
70
2nd
Larval
Instar
60
50
3rd
Larval
Instar
40
30
4th
Larval
Instar
20
10
0
7
15
30
45
Storage Periods (Days)
60
Adults Emerged from
Stored Pupae (%)
Pupae Success Rate
90
80
70
60
50
40
30
20
10
0
7
15
30
45
Storage Periods (Days)
60
Adults Success Rate
100
Survival of Adults (%)
90
80
Emergence
70
60
5 Days
50
40
10 Days
30
20
10
0
7
15
30
45
Storage Periods (Days)
60
Adults Success Rate
100
Survival of Adults (%)
90
80
Emergence
70
60
5 Days
50
40
10 Days
30
20
10
0
7
15
30
45
Storage Periods (Days)
60
Adults Success Rate
100
Survival of Adults (%)
90
80
Emergence
70
60
5 Days
50
40
10 Days
30
20
10
0
7
15
30
45
Storage Periods (Days)
60
Final Thoughts
• Eggs, Larval Instars & Pupae Survived
Short-Term Storage, 7 Days
• Adult Storage More Long-Term, 45 Days
Due to Diapause
• Simple Method for Storing Large Numbers
• Easy Shipping
• Synchronization With Insect Pest
• Suppress Insect Pests When Required
In Conclusion
• Cold Stored
• Fed Adults Are Most Receptive
• Biological Control Agents