Transcript Slide 1

Introducing the DSQII
Terry Jeffers
Agenda
• Update on Thermo’s Environmental Initiatives
• Discussion on new approaches to the challenges of 8270
• Introducing Thermo Electrons Productivity Kits
• EnviroLab Forms 2.0 – High Throughput Quatification
• New application note: Rapid Analysis of 8270
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Thermo Electron: Environmental Initiative
• Focus on Environmental Laboratory needs:
– Requirement for Productivity
– Need for good work flow in Generating, Reviewing and Reporting
data
– Benefit from guides for operation, maintenance and troubleshooting
specific Methodologies
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Thermo Electron: Environmental Initiative
Product Sales Specialist
Environmental Demo Chemist
Environmental Seed Chemist
New hardware: DSQ II
Productivity Kits:
8270 and 525
8260 and 524
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*
Pitt Falls for Method 8270
Those little things that stop everything
Activity in the GC/MS system
• Activity
– These sites can be everywhere
• Injector, column, source
• SPCC compounds are used to show good system
– Nitrophenols are some of the best compounds to find active sites
• Minimizing Effects of Activity
– Deactivated liners
– Good column that shows no active sites
– High flow rates to reduce the residence time of any active
compound.
– Balance temperature zones to reduce activity and thermal break
down.
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Chromatography
How important is it really?
GC and Autosampler
• Separations accomplished with slow chromatography
– Common GC methods for 8270
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Fast injection
250 - 300 0 C injector temperature
1 mL/min flow rate
30 m x 0.25 mm ID column
Oven ramps between 5 0 and 10 0 C/min from 35 0 to 300 0 C
• Separations accomplished with fast chromatography
– Alternate GC method for 8270
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•
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Hot needle injection
250 0 C injector temperature
3 mL/min flow rate
30 m x 0.25 mm ID x 0.5 µm column
Oven stepped ramps to provide the critical pair separations.
Hot Needle Injection: Run at Lower Inlet
Temperature: Fewer Replacements of Liner
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Increase the Mobile Phase:
3 mL/min vs 1mL/min
• Increase capacity of stationary
– No fronting of peak shape for 160 ng/uL PNA
• Decrease the irreversible absorption of active species
– Pentachlorophenol < 10%RSD
• (5-160 ng/uL)
• 0.1 ng/uL MDL
– replicates at 1 ng/uL 4.7%RSD
– Minimal breakdown for endrin or 4,4-DDT
• Provide Gaussian shape peaks for the most volatile
– No solvent skewing of NDMA and pyridine
• Shorter run times: 17 minutes
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Effects of increased flow rates
• Why increase the flow rate to 3.0 mL/min
– Learned from applications developed for the analysis of explosives.
– Decrease the residence time of the compounds in potential active
areas.
– Decreases the run time without higher temperatures that could
potentially damage thermally labile compounds.
– Do not need to expose the column to higher temperatures that will
damage it.
– Provides better separations and narrower peak widths.
– This is balanced with a 20:1 split flow ratio.
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Injection: Choice of Liner
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PNAs ( 5 ng and 160 ng)
RT: 17.32 - 18.34
RT: 17.45
AA: 1357507
BP: 276.1
100
NL:
4.27E5
m/z=
275.5276.5 F:
MS ICIS
031706_b_0
03
RT: 18.05
AA: 1192699
BP: 276.1
80
60
40
20
0
100
RT: 17.47
AA: 38042327
BP: 276.1
NL:
1.14E7
m/z=
275.5276.5 F:
MS ICIS
031706_b_0
08
RT: 18.07
AA: 32375912
BP: 276.1
80
60
40
20
0
17.4
13
17.5
17.6
17.7
17.8
17.9
Time (min)
18.0
18.1
18.2
18.3
NDMA and Pyridine
RT: 1.71 - 5.17
Split
100
80
4.94 5.05 NL:
3.81
3.19
2.46
1.79E8
TIC F: MS
C07
60
4.10
4.45
2.42
40
3.29
20
2.71
3.12
0
100
3.62
3.44
3.97
4.13
4.50 4.84
4.99
Splitless
80
60
4.87
40
20
4.52
0
2.0
14
2.5
3.0
3.5
Time (min)
4.0
4.5
5.0
NL:
8.09E6
TIC F: MS
A07
Two Methods: Splitless and Split
RT: 0.00 - 30.08
5.85
100
9.50
80
12.89
8.92
8.72
60
40
5.05
NL:
5.80E8
TIC F: MS
C07
13.53
14.74
5.40
15.18
17.10
20
17.87 19.87 21.84
0
100
NL:
1.37E8
TIC F: MS
A07
9.42
80
8.10
60
8.22
7.49
40
12.12
11.32
7.34
13.34
14.74 15.10
15.82
6.37 6.90
4.87
20
17.41
18.73 22.26
25.26
26.19
0
0
15
5
10
15
Time (min)
20
25
30
TRACE Ultra DSQ II GC/MS
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Source materials and geometry
• New DuraBriteTM Source Open Geometry
– Passive Collector
• Extends Dynamic Range
• Improves Robustness
– Optional Closed EI Ion Volume
• Constructed of new proprietary material for enhanced
inertness
• Protects Lens 1 from ion burn
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New DynaMax XR Ion Detection System
SGE ETP AF635 Electron Multiplier
• Detector linear range
output current >30µA
• Simple snap out replacement of
multiplier assembly
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New Linear/Log Hybrid Electrometer
• Extends output current to 70µA
• Provides dynamic range to >106
• No loss of response or precision at the lower
sample/analyte levels
Expected Results
Results I can get, you can get them too.
Calibration Curve
• First page of the calibration
curve report
• All SPCC and CCC
requirements met
• Average of all RSD calibration
curves of 7.46%
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SPCCs (5ng to 160 ng min >0.05)
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2,4-dinitrophenol
0.11
4-nitrophenol
0.19
hexachlorocyclopentadiene
0.212
N-nitroso-di-n-propylamine
0.688
CCCs < 30% RSD for Initial Calibration Curve
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BNAs
%RSD
Acids
%RSD
1,4-dichlorobenzene
2.2
2,4-dichlorophenol
8.6
acenaphthene
1.6
3.9
benzo(a)pyrene
7.1
4-chloro-3methylphenol
2-chlorophenol
Di-n-octylphthalate
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pentachlorophenol
9.1
diphenylamine
1.9
phenol
4.7
fluoranthene
3.8
2,4,6-trichlorophenol
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hexachlorobutadiene
2.6
2-nitrophenol
8.5
5.1
Quantitated Data File
• First page of the Quantitation
Report
• This shows the quantitation of
the 20 ng/uL sample for the
calibration curve
• Meets the requirements of the
internal standards and
surrogates
• Meets all ion ratio requirements
• No manual integrations were
required
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Robustness: Split (2gr sediment/2mL methylene
chloride + 25 uL diesel fuel)
RT: 0.00 - 17.11
8.33
100
7.77
90
Relative Abundance
9.36
9.83
7.18
80
70
10.28
10.71
6.53
5.01 5.82
60
NL:
1.08E9
TIC F: MS
rep96
8.87
11.12
4.04
50
11.51
40
30
11.90
2.34
3.97
12.26
20
12.61
12.96
1.40 1.70
10
14.00 14.87
0
0
2
4
6
8
Time (min)
10
12
2.5000
2.0000
1.5000
1.0000
0.5000
0.0000
1 3 5 7 9 11 13 15 17 19 21 23 25
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14
16
Phenol(CCC) 2%
1,4-Dichlorobenzene(CCC) 1%
2-Nitrophenol(CCC) 2%
2,4-Dichlorophenol(CCC) 1%
Hexachlorobutadiene(CCC) 2%
4-Chloro-3-methylphenol(CCC) 2%
2,4,6-Trichlorophenol(CCC) 1 %
Acenaphthene(CCC) 1%
Pentachlorobenzene(CCC) 2%
Diphenylamine(CCC) 2%
Pentachlorophenol(CCC) 2%
Fluoranthene(CCC) 2%
Di-n-octylphthalate(CCC) 3%
Benzo(a)pyrene(CCC) 1%
How can we prove it
Productivity Solutions
Productivity Solutions
Integrating the DSQ II with Software, Methods,
and Consumables to Get Productive, Quickly
Productivity Solutions
• Provide all of the necessary material to run the method
– Proven Thermo Column
– Deactivated Liners
– Standards
• Snap and shoot – prediluted for entire method validation
– All other consumables for about 2 weeks
• Provide all of the software
– EnviroLab Forms 2.0
– Interactive Reference CD
• All of the methods, sequences,
– Validation data used to generate all of what you see here and in the
manuals
• Two Boxes full of materials, manuals, and CD’s.
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Productivity Kit
• SOP manual for method
• How to Guide
• Quick Start Guide
• Provide consumables for running method
– Standards, liners, septa, syringes, column,…
• Instrument method, processing method, reporting package
• CD deliverable
– SOP
– Data from method validation
– Instrument method
– Processing method
– Reporting software with templates
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Pieces of the Productivity Kit
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Quick Start Guide
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8270C/D Productivity Solution
• Customer’s system can be taken from installation sign off to
method IDL’s in as little as 2 days!
• 8270 is a value added product which will sell for ~ $3000 US
• Add on service provided by Thermo FSE’s
• US Field Service Engineers are being trained on the 8270 PS
• Kits serve as a great tool for training aides for the lab
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Productivity Solutions are a Win-Win
• Good for customers –
– Improves overall experience
– Increases satisfaction
– Eases transition to Thermo GC/MS
– Gives optimal performance for the DSQ II
• Good for Thermo
– Easy to support these solutions
Helps the customer and support by establishing a known
set of criteria to achieve success.
Address the new lab environment less highly skilled users
with higher throughput requirements
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Redefining the Way We Look at GC/MS
Hardware
Productivity Solutions target routine GC/MS applications to offer value to
the customer
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EnviroLab Forms v 2.0
High Throughput Quantification
EnviroLab Forms v 2.0
• An entirely new data processing and reporting system running
as a layer over Xcalibur 1.4
• While targeted towards environmental laboratories and
applications, it has been shown to be valuable for any
laboratory performing high-throughput quantification
• Adds a significant complement of capabilities that Xcalibur
– Ion ratio calculations
• Peak integration
• Reporting
– Integrated data review and reporting
• No more batch reprocessing to re-generate reports of edited results
– Fully automated while still allowing sophisticated manual review
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Data Review
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Report View
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EnviroLab Forms v 2.0
• Brand new workflow oriented user interface
• Substantially improved ion ratio review and editing support
• Comprehensive set of textual and graphical reports
• Fully automated, fully manual or any combination in between
• Can be used as a high-throughput quantification engine for
any application
– Simply turn off or don’t use those options which aren’t relevant for a
given user
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Conclusion
• The DSQ II system provides updates to hardware and
software that make it easy to use
• The Productivity Solution for method 8270 provides a method
that has been validated across multiple DSQ II systems.
• All of this allows a new operator to be productive immediately
• The proven changes to the method allow an increased amount
of productivity and throughput for the analyst and the
laboratory.
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•Questions?
Thank You
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