Transcript UVStar

®
UVStar
Unique Microplates for
the UV-Range
Pre-UVStar®-Period
At my time,
only quartz glass was
available, and it was

fragile

expensive Microplates

hard to clean for reuse

limited in throughput
2
Uniqueness of UVStar®

Microplates with outstanding optical properties

Dedicated for OD reading in the UV-range
 Proprietary,

unique resin
Optical window down to 200 nm
 Available
in 96 and 384 well format
3
Prerequisites for UVStar®
 Availability
 Material
of a unique proprietary plastic
free of any UV-blocking additives
 Production
of high quality films (140 µm)
 Lamination
process free of glue and solvents
 Adaptation
of µClear®-process to UVStar®
4
µClear®-Technology

Direct lamination technology

Process free of glue or solvents

50 - 500 µm films

Enables multi-mode detection
Worldwide Patents
Germany:
DE 19712484
Europe:
Pat. pending
USA:
Pat. pending
5
Applications of UVStar®-Plates
 Optical
window down to 200 nm
 Background:
< 60 mE @ 260 nm
 DNA-quantification
 Purity
@ 260 nm
of DNA @ 260/280 nm
 Protein
concentration @ 280 nm
6
Absorbance of DNA vs. Protein
4
Protein
3
OD
DNA
2
1
0
240
245
250
255
260
265
270
wavelength [nm]
7
275
280
285
290
Clear Bottom Plates
1 mm bottom
140 µm bottom
 bonding of glass sheets
 2 component injection molding
 bonding of plastic films
8
Advantages of µClear®-Technology
120
transmittance [%]
100
80
µClear®
60
PS 150 µm
40
PS 1 mm
20
0
200
250
300
350
wavelength [nm]
9
400
450
500
From µClear® to UVStar®
120
transmittance [%]
100
80
UVStar®
60
UV-resin #2
40
µClear®
UV-resin #1
20
0
200
250
300
350
wavelength [nm]
10
400
450
500
Absorbance of UV-transparent Resins
Greiner vs. Competitors
2,0
1,6
Greiner: 140 µm
Competitor A: 100 µm
1,2
OD
Competitor C: 190 µm
0,8
0,4
0,0
200
220
240
260
wavelength [nm]
11
280
300
UV-Light Absorbance
384 well µClear® vs. UVStar®
2,8
2,4
absorbance [E]
2,0
1,6
UVStar®
1,2
µClear®
0,8
0,4
0,0
200
250
300
350
wavelength [nm]
12
400
450
500
Measurement of DNA-Conc.
96 vs. 384 Well UVStar®-Plates
2,0
2
R = 0,9931
R2 = 0,9992
absorbance [E]
1,6
1,2
96 well
0,8
384 well
0,4
0,0
0
20
40
60
conc. DNA [µg/mL]
13
80
100
120
Cell Growth (WST)
in TC-treated 384 Well UVStar®-Plates
4
absorbance [E]
3
C127
2
L929
Expon
entiell
(L929)
Expon
entiell
(C127)
1
0
1
2
3
days post seeding
14
4
Growth of CHO-Cells
in TC-treated 96 Well Plates
10.000
fluorescence [RFU]
8.000
UVStar®
6.000
CELLSTAR®
4.000
2.000
0
1
2
3
days post seeding
15
4
7
Homogeneity of 384 Well Plates
0,100
absorbance [E]
0,080
232 nm
280 nm
260 nm
340 nm
0,060
0,040
0,020
0,000
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
column
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Performance of Background
384 Well UVStar® @ 260 nm
0,060
absorbance [E]
0,050
0,040
0,030
P
M
0,020
J
G
0,010
D
A
0,000
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
column
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Drift and Background
Greiner vs. Competitor
0,055
Competitor C
0,054
OD @ 260 nm
UVStar®
0,053
0,052
0,051
0,050
0,049
1
2
3
4
5
7
6
Row
18
8
9
10
11
12
Drift and Background
Greiner vs. Competitor
Competitor
0,062
Greiner
0,062
0,0580-0,0620
0,0580-0,0620
0,0540-0,0580
0,0540-0,0580
0,0500-0,0540
0,0460-0,0500
0,058
0,0500-0,0540
0,058
0,0460-0,0500
0,054
0,054
0,050
0,050
R7
R7
0,046
0,046
1
2
R4
3
4
5
6
7
8
9
10
11
1
R1
12
Mean
Mean
0,0524
Standard Deviation
0,002
Correlation of Variation 4 %
2
R4
3
4
5
6
7
8
9
10
11
R1
12
0,0498
Standard Deviation
0,001
Correlation of Variation 2 %
19
Pathlength & Lambert Beer’s Law
I = Io ·e-k’ · c · x
Io
Io
I
X
I
X
Well pathlength [x]:
Cuvette pathlength [x]:
not defined
fixed
20
Correlation Pathlength vs. Absorption
@ 260 nm
Quartz Cuvette
[10 mm] = 0,08 OD
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Pathlenght [mm]
OD
0.8 mm
0,0059
2.0 mm
0,0200
4.0 mm
0,0516
6.0 mm
0,0578
8.0 mm
0,095
10.0 mm
0,0828
Chemical and Thermal Resistance
Chemical / Temperature
UVStar®
+ 40oC / - 80oC
resistant
> 40oC
non-resistant
DMSO
resistant
Ethanol 96 %
resistant
Methanol
resistant
HCl (36 %)
resistant
H2SO4 (40 %)
resistant
Pentane
non-resistant
Heptane
non-resistant
Benzene
non-resistant
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Benefits of UVStar®-Plates
 Available
in a 96 well and 384 well format
 Extended
optical window from 200 to 1000 nm
 Background
 Minimized
 Ideal
 No
less than 0.060 E @ 260 nm
variation from well to well
for quantifying DNA and total protein
more need for expensive/fragile quartz plates
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®
UVStar
Made by