Transcript Kein Folientitel

Cytokine production of human
preimplantation embryos
M. H. Özörnek, P. Bielfeld, U. Koldovsky
Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany
IFN gamma and IL-10 levels
on day 1 vs. day 2
I
U
/
m
l
(
I
F
N
)

p g /ml ( IL - 1
0
)
4 0
2
3 0
1 .5
IF N
2 0
1
IL -
1 0
0 .5
0
d a y
1
0
d a y
IFN gamma levels before
implantation
IU/ml
1
0,8
0,6
0,4
0,2
0
day 2
day 3
day 4
day 5
day 2
day 3
day 4
day 5
 Human
embryo do not produce
CSF-1 and LIF,
at least 2-5 days after fertilisation.
EGF production of human embryo
1.5
pg/ml
1
0.5
0
day d
2
ay d
3
ay d
4
ay
5
Importants of critical selection of
recurrent pregnancy loss patients
for successful immunotherapy
M. H. Özörnek, B. Mikat-Drodzdynski,
U. Koldovsky
Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany
Conditions for Immunotherapy
University of Düsseldorf
Primary recurrent pregnancy loss
 Absence of "classical" abortion reasons
 Absence of an autoimmune pathology
 negativ APCA and EAI tests
 negativ infection parameters (HIV,HBsAg,Anti HCV,TPHA,CMV)

Immunodiagnostik

Antipaternal Cytotoxic Antibody (APCA) [crossmatch]
A test for prooving the presence of cytotoxic
antibodies in patient serum against partner
lymphocytes

Erythrocyt Antibody Rosette Inhibition (EAI) Test
A test for prooving the Fc receptor blocking
antibodies
Protocol of Immunotherapy
E
A
I
a
n
d
A
P
C
A
p
o
s
i
t
i
v
n
e
g
a
t
i
v
n
o
t
h
e
r
a
p
y
i
m
m
u
n
i
z
a
t
i
o
n
r
e
p
e
a
d
E
A
I
a
n
d
A
P
C
A
a
f
t
e
r
4
6
w
e
e
k
s
p
o
s
i
t
i
v
n
e
g
a
t
i
v
n
o
t
h
e
r
a
p
y
2
.
i
m
m
u
n
i
z
a
t
i
o
n
Immunotherapy results
I
m
m
u
n
i
z
e
d
p
a
t
i
e
n
t
s
:
3
6
(
T
o
t
a
l
6
2
i
m
m
u
n
i
z
a
t
i
o
n
s
)
T
o
t
a
l
p
r
e
g
n
a
n
c
y
:
2
8
2
2
D
e
l
i
v
e
r
y
6
A
b
o
t
i
o
n
s
(
2
g
e
n
e
t
i
c
o
r
i
g
i
n
)
L
i
v
e
b
i
r
t
h
r
a
t
e
(
2
2
/
2
8
)
:
7
8
.
6
%
Conclusion
 If
RPL is primary,
 If all losses are in 1. Trimester,
 If there are no autoimmune antibodies,
 If both EAI and APCA tests are negativ
Immunization is a realistic therapy!