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Cytokine production of human
preimplantation embryos
M. H. Özörnek, P. Bielfeld, U. Koldovsky
Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany
IFN gamma and IL-10 levels
on day 1 vs. day 2
I
U
/
m
l
(
I
F
N
)
p g /ml ( IL - 1
0
)
4 0
2
3 0
1 .5
IF N
2 0
1
IL -
1 0
0 .5
0
d a y
1
0
d a y
IFN gamma levels before
implantation
IU/ml
1
0,8
0,6
0,4
0,2
0
day 2
day 3
day 4
day 5
day 2
day 3
day 4
day 5
Human
embryo do not produce
CSF-1 and LIF,
at least 2-5 days after fertilisation.
EGF production of human embryo
1.5
pg/ml
1
0.5
0
day d
2
ay d
3
ay d
4
ay
5
Importants of critical selection of
recurrent pregnancy loss patients
for successful immunotherapy
M. H. Özörnek, B. Mikat-Drodzdynski,
U. Koldovsky
Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany
Conditions for Immunotherapy
University of Düsseldorf
Primary recurrent pregnancy loss
Absence of "classical" abortion reasons
Absence of an autoimmune pathology
negativ APCA and EAI tests
negativ infection parameters (HIV,HBsAg,Anti HCV,TPHA,CMV)
Immunodiagnostik
Antipaternal Cytotoxic Antibody (APCA) [crossmatch]
A test for prooving the presence of cytotoxic
antibodies in patient serum against partner
lymphocytes
Erythrocyt Antibody Rosette Inhibition (EAI) Test
A test for prooving the Fc receptor blocking
antibodies
Protocol of Immunotherapy
E
A
I
a
n
d
A
P
C
A
p
o
s
i
t
i
v
n
e
g
a
t
i
v
n
o
t
h
e
r
a
p
y
i
m
m
u
n
i
z
a
t
i
o
n
r
e
p
e
a
d
E
A
I
a
n
d
A
P
C
A
a
f
t
e
r
4
6
w
e
e
k
s
p
o
s
i
t
i
v
n
e
g
a
t
i
v
n
o
t
h
e
r
a
p
y
2
.
i
m
m
u
n
i
z
a
t
i
o
n
Immunotherapy results
I
m
m
u
n
i
z
e
d
p
a
t
i
e
n
t
s
:
3
6
(
T
o
t
a
l
6
2
i
m
m
u
n
i
z
a
t
i
o
n
s
)
T
o
t
a
l
p
r
e
g
n
a
n
c
y
:
2
8
2
2
D
e
l
i
v
e
r
y
6
A
b
o
t
i
o
n
s
(
2
g
e
n
e
t
i
c
o
r
i
g
i
n
)
L
i
v
e
b
i
r
t
h
r
a
t
e
(
2
2
/
2
8
)
:
7
8
.
6
%
Conclusion
If
RPL is primary,
If all losses are in 1. Trimester,
If there are no autoimmune antibodies,
If both EAI and APCA tests are negativ
Immunization is a realistic therapy!