Transcript Principle of Operation: ALZET cross

ALZET Speakers Kit
ALZET Osmotic Pumps
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ALZET Osmotic Pump Advantages
Ensure around-the-clock exposure to test agents at predictable levels
Permit continuous administration of short half-life proteins and peptides
Convenient method for chronic dosing of laboratory animals
Minimize unwanted experimental variables and ensure reproducible,
consistent results
Eliminate the need for nighttime or weekend dosing
Reduce handling and stress to laboratory animals
Small enough for use in mice or very young rats
Allow for targeted delivery of agents to virtually any tissue
Cost-effective research tool
ALZET Osmotic Pumps
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Injection vs. Infusion
ALZET Osmotic Pumps
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Principle of Operation
ALZET pumps have 3 concentric layers:
 Rate-controlling, semi-permeable
membrane
 Osmotic layer
 Impermeable drug reservoir
ALZET pumps work by osmotic
displacement. Water enters the pump
across the outer, semi-permeable
membrane due to the presence of a high
concentration of sodium chloride in the
osmotic chamber. The entry of water
causes the osmotic chamber to expand,
thereby compressing the flexible reservoir
and delivering the drug solution through
the delivery portal.
ALZET Osmotic Pumps
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ALZET Osmotic Pumps
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ALZET® osmotic pumps are available
in three sizes
ALZET Osmotic Pumps
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ALZET Brain Infusion Kits
Together with ALZET pumps, the Brain
Infusion Kits permit the continuous delivery
of test compounds to the CNS for extended
periods of time without external connections,
restraints, or frequent handling of laboratory
animals.
ALZET Osmotic Pumps
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Specifications of ALZET Brain Kits
Brain Kits 1 & 2
Brain Kit 3
Brain Infusion Cannula
Material (tube)
Dimensions (steel tube)
Material (elbow stop, flange)
Stainless steel
28 gauge
30 gauge
ID = 0.18 mm; OD = 0.36 mm
ID = 0.16 mm; OD = 0.31 mm
Material (elbow stop, flange)
Volume inside tube
Polycarbonate
0.32 µl
0.23µl
Height Adjustment Spacers
Material
Polycarbonate
Dimensions (height)
0.5 mm
Catheter Tubing
Material
Polyvinylchloride (Medical grade)
Length
15 cm (approx.)
Inside diameter
0.69 mm (± 0.08)
Outside diameter
1.14 mm (± 0.08)
Volume per 15 cm
56 µl (3.7 µl/cm)
ALZET Osmotic Pumps
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ALZET Brain Infusion Kits
ALZET Brain Infusion Kits are designed for targeted delivery to the cerebral
ventricles or direct microperfusion of discrete brain structures. Available kits
have slightly different cannula designs.
ALZET Osmotic Pumps
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In vitro vs. in vivo pumping rate
The typical pumping rate of the Model 2ML4 ALZET Osmotic Pump over time
in Sprague Dawley rats after subcutaneous implantation (n=105)
ALZET Osmotic Pumps
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Typical pumping rate of the Model 2002 ALZET
Osmotic Pump over time (n-20)
ALZET Osmotic Pumps
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ALZET Contact Information
ALZET Customer Service
Mailing Address
(Pricing and Ordering)
DURECT Corporation
P.O. Box 530
Cupertino, CA. 95015-0530

Toll free: 1-877-922-5938
 Phone: 1-408-253-8574
 Fax: 1-408-865-1406
ALZET Technical Support

Toll free: 1-800-692-2990
 Phone: 1-408-367-4036
 Fax: 1-408-865-1406
ALZET Osmotic Pumps
Web Address
www.alzet.com
www.alzet.com