Transcript The Effect of Artificial Sugar and Raw Sugar on Daphnia’s

The Effect of Artificial Sugar
and Raw Sugar on the
Daphnia’s Heartbeat
Katie Clarke
Nov. 7 2006
Purpose
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To see how the daphnia’s heartbeat is
affected by the artificial sugar and the
raw sugar.
Background- Daphnia
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Over 150 species
Males- 2 millimeters
Females- 3-5
millimeters
Diet of algae
Background- Artificial
Sugar (Aspartame)
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Aspartic Acid
40%
an excitotoxin
Can pass through the blood brain
barrier
Background- Aspartame
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Phenylalanine
50%
4% is in protein we eat
Background- Aspartame
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Methyl Ester
10%
Wood Alcohol
Breaks down into formaldehyde
Background- Raw Sugar
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Not processed
From crushed sugar canes
Thin coating of molasses
Hypothesis
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If the daphnia is exposed to artificial
sugar containing aspartame the
daphnia’s heartbeat will be higher than
it would be with raw sugar.
Variables
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Dependant Variable- Daphnia’s
heartbeat
Independent Variable- Artificial sugar
and Raw sugar
Control
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Control- Daphnia’s heartbeats with
water
Constants
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Constants- The daphnia, the amount
of raw sugar used, the amount of
artificial sugar used, and the time each
daphnia was watched.
Materials
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2 Cultures of
Daphnia Magna
1 Teaspoon
1 Microscope
1 Slide
Artificial Sugar
“Equal”
Raw Sugar “Sugar
in the Raw”
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3 Eyedroppers
2 Cups of water
1 Watch
Procedure
Results
Daphnia's Heartbeats per Minute
Daphnia Heartbeats
600
500
400
Water
300
Equal
200
Sugar in the Raw
100
0
1
2
3
Minutes
4
5
Results
Daphnia's Average Heartbeat
Minutes
1
2
3
4
5
Water
218
245
217
267
270
Artificial Sugar
312
502
299
306
304
Raw Sugar
290
293
301
296
298
Conclusion
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To see how the daphnia’s heartbeat is
affected by the artificial sugar and the
raw sugar.
The Hypothesis was accepted
Possible because of the excitotoxin
Aspartame affects the brain more than
the heart.
Used in Real Life
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To learn more about what we eat.
Recommendations
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Learn more about how it affects the
heart
Make the project last longer
Acknowledge
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My parents
J.T. and Cori
Mrs. Peffley