Transcript Slide 1
POWERPOINT® LECTURE SLIDE PRESENTATION
by LYNN CIALDELLA, MA, MBA, The University of Texas at Austin
UNIT 1
5
PART B
Membrane Dynamics
HUMAN PHYSIOLOGY
AN INTEGRATED APPROACH
DEE UNGLAUB SILVERTHORN
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FOURTH EDITION
Carrier-Mediated Transport
Specificity
Competition
Saturation
Transport maximum
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Vesicular Transport
Phagocytosis
Cell engulfs bacterium or other particle into
phagosome
Endocytosis
Membrane surface indents and forms vesicles
Active process which can be nonselective
(pinocytosis) or highly selective
Potocytosis uses caveolae
Receptor-mediated uses clathrin-coated pits
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Receptor-Mediated Endocytosis
and Exocytosis
1 Ligand binds to membrane receptor.
9 Exocytosis
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
8 Transport vesicle
and cell membrane
fuse (membrane
recycling).
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
7 Transport vesicle
with receptors moves
to the cell membrane.
5 Receptors
and ligands
separate.
To lysosome or
Golgi complex
6 Ligands go to lysosomes
or Golgi for processing.
Endosome
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44 Vesicle loses
clathrin coat.
Intracellular fluid
Figure 5-24
Receptor-Mediated Endocytosis
and Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
Receptor
Intracellular fluid
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Figure 5-24, step 1
Receptor-Mediated Endocytosis
and Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
Receptor
Clathrin
Intracellular fluid
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Figure 5-24, steps 1–2
Receptor-Mediated Endocytosis
and Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
Intracellular fluid
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Figure 5-24, steps 1–3
Receptor-Mediated Endocytosis
and Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
44 Vesicle loses
clathrin coat.
Intracellular fluid
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Figure 5-24, steps 1–4
Receptor-Mediated Endocytosis and
Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
5 Receptors
and ligands
separate.
Endosome
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44 Vesicle loses
clathrin coat.
Intracellular fluid
Figure 5-24, steps 1–5
Receptor-Mediated Endocytosis and
Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
5 Receptors
and ligands
separate.
To lysosome or
Golgi complex
6 Ligands go to lysosomes
or Golgi for processing.
Endosome
Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings
44 Vesicle loses
clathrin coat.
Intracellular fluid
Figure 5-24, steps 1–6
Receptor-Mediated Endocytosis and
Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
7 Transport vesicle
with receptors moves
to the cell membrane.
5 Receptors
and ligands
separate.
To lysosome or
Golgi complex
6 Ligands go to lysosomes
or Golgi for processing.
Endosome
Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings
44 Vesicle loses
clathrin coat.
Intracellular fluid
Figure 5-24, steps 1–7
Receptor-Mediated Endocytosis and
Exocytosis
1 Ligand binds to membrane receptor.
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
8 Transport vesicle
and cell membrane
fuse (membrane
recycling).
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
7 Transport vesicle
with receptors moves
to the cell membrane.
5 Receptors
and ligands
separate.
To lysosome or
Golgi complex
6 Ligands go to lysosomes
or Golgi for processing.
Endosome
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44 Vesicle loses
clathrin coat.
Intracellular fluid
Figure 5-24, steps 1–8
Receptor-Mediated Endocytosis and
Exocytosis
1 Ligand binds to membrane receptor.
9 Exocytosis
Extracellular fluid
2 Receptor-ligand migrates to
clathrin-coated pit.
8 Transport vesicle
and cell membrane
fuse (membrane
recycling).
Clathrincoated pit
3 Endocytosis
Receptor
Clathrin
7 Transport vesicle
with receptors moves
to the cell membrane.
5 Receptors
and ligands
separate.
To lysosome or
Golgi complex
6 Ligands go to lysosomes
or Golgi for processing.
Endosome
44 Vesicle loses
clathrin coat.
Intracellular fluid
Exocytosis is the opposite of endocytosis
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Figure 5-24, steps 1–9
Transepithelial Transport
Polarized cells of transporting epithelia
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Figure 5-25
Transepithelial Transport of Glucose
[Glucose] low
Lumen of kidney
or intestine
Na+ [Na+] high
Glu
1
1 Na+ glucose symporter
brings glucose into cell
against its gradient using
energy stored in the Na+
concentration gradient.
2 GLUT transporter
transfers glucose to ECF
by facilitated diffusion.
Apical
membrane
[Glucose] high
Glu
Na+ [Na+] low
Epithelial
cell
Basolateral
membrane
Glu
Na+
3 Na+ -K+- ATPase pumps Na+
out of the cell, keeping ICF
Na+ concentration low.
K+
2
3
ATP
Extracellular
fluid
[Glucose] low
Glu
[Na+] high Na+
K+
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Figure 5-26
Transepithelial Transport of Glucose
Lumen of kidney
or intestine
[Glucose] low
Glu
Na+ [Na+] high
1
1
Na+ glucose symporter
brings glucose into cell
against its gradient using
energy stored in the Na+
concentration gradient.
Apical
membrane
[Glucose] high
Glu
Na+ [Na+] low
Epithelial
cell
Basolateral
membrane
Extracellular
fluid
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Figure 5-26, step 1
Transepithelial Transport of Glucose
[Glucose] low
Lumen of kidney
or intestine
Glu
Na+ [Na+] high
1
1 Na+ glucose symporter
brings glucose into cell
against its gradient using
energy stored in the Na+
concentration gradient.
2 GLUT transporter
transfers glucose to ECF
by facilitated diffusion.
Apical
membrane
[Glucose] high
Glu
Na+ [Na+] low
Epithelial
cell
Basolateral
membrane
Glu
2
Extracellular
fluid
[Glucose] low
Glu
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Figure 5-26, steps 1–2
Transepithelial Transport of Glucose
[Glucose] low
Lumen of kidney
or intestine
Na+ [Na+] high
Glu
1
1 Na+ glucose symporter
brings glucose into cell
against its gradient using
energy stored in the Na+
concentration gradient.
2 GLUT transporter
transfers glucose to ECF
by facilitated diffusion.
Apical
membrane
[Glucose] high
Glu
Na+ [Na+] low
Epithelial
cell
Basolateral
membrane
Glu
Na+
3 Na+ -K+- ATPase pumps Na+
out of the cell, keeping ICF
Na+ concentration low.
K+
2
3
ATP
Extracellular
fluid
[Glucose] low
Glu
[Na+] high Na+
K+
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Figure 5-26, steps 1–3
Transcytosis across the Capillary Endothelium
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Figure 5-27
Transcytosis across the Capillary Endothelium
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Figure 5-27 (1 of 3)
Transcytosis across the Capillary Endothelium
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Figure 5-27 (2 of 3)
Transcytosis across the Capillary Endothelium
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Figure 5-27 (3 of 3)
Transcytosis across the Capillary Endothelium
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Figure 5-27