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Collecting P. philadelphica in central-southern Mexico Research purposes • To study the genetics of fruit size increase in tomatillo occurred under domestication. This will be carried out by association mapping • To study the population structure in tomatillo: results are useful for association mapping • To study the phylogeographic history of tomatillo • To Investigate where and how cultivated forms where domesticated: history of domestication Jalisco Puebla Guerrero Oaxaca Chiapas Sources of collection of germplasm • • • • • Undisturbed Vegetation Disturbed vegetation: Wild forms or “tomate de milpa” Maize Fields: Wild forms or “tomate de milpa Cultivated fields: small-fruited and large-fruited varieties Traditional markets: Wild forms, small and large-fruited varieties • Farmer’s stock: small and large-fruited varieties Other Physalis species collected: Physalis ampla Physalis angulata Physalis ampla Physalis angulata Variation in fruit size Wild Domesticated “Tomate de milpa” grows in maize fields called milpas (Jalisco) Cultivated field in Puebla Tomatillo is very abundant today in traditional markets in Mexico. It is the fifth most important vegetable species cultivated in this country 23579 Ha were cultivated in 1990 Women harvest the fruits (November, Puebla) Women process seeds for next growing season (Puebla) Fruits are stored in boxes Ready to be sold Diseases: Coleoptera eat the leaves Ethnobotany of the husk tomato . The word “tomate” comes from the Nahuatl indigenous word “Ayacach tomatl” and means berry. . Local names: Miltomate (oax), tomate verde (Jal), tomatillo (Jalisco, Oax, Puebla), tomate de cascara (Jal, Puebla, Oax, Chiapas), tomate de hoja (Jalisco, Puebla). . Aztecs cultivated the husk tomato with maize and used to call it “miltomate” that means “tomate de milli” or cultivated tomato with maize. It was used for cooking and medicinal purposes. Ethnobotany of the husk tomato Uses: Salsa Verde Infusion of calix to make the dough for tamales Leaves and fruits to treat headaches and stomachaches Juice to treat the sore throats Cooked calix to treat diabetes Sowing and Harvest Dates Jalisco Puebla Oaxaca Chiapas Sowing Harvest June July August-September September August-September September-October October-November November Number of germplasm collections available at BANGEV, USDA and the new collections State Baja California Chiapas Chihuahua Colima Guanajuato Guerrero Hidalgo Jalisco México Michoacán Morelos Nayarit Oaxaca Puebla San Luis Potosí Sonora Zacatecas Total BANGEV No. of No. of accessions collection sites 3 1 USDA No. of accessions - No. of collection sites - New Collections No. of No. of accessions collection sites - 1 1 7 7 4 74 26 21 9 9 100 125 1 1 1 6 4 3 45 10 14 6 7 1 16 1 8 4 - 5 4 - 22 13 2 13 3 39 11 - 18 11 1 13 2 37 8 - 1 2 391 1 2 119 12 9 105 90 Number of wild and landrace collections of P. philadelphica per state 40 Number of collections 35 30 25 20 15 10 5 0 Jalisco Michoacan Guerrero Hidalgo State wild landrace Puebla Oaxaca Chiapas Main Findings New collections mainly from under-collected states: Oaxaca and Chiapas Great variation in fruit size exist along the range of distribution Truly wild forms were not found. Wild forms are commonly associated with maize or grow in disturbed habitats near cultivated fields Local preferences in fruit type: Purple fruits in Jalisco, big and green fruits in Puebla, yellow fruits in Guerrero, and small and purple fruits in Chiapas The crop is still grown on a traditional agricultural system