Transcript MICHAEL HOOKER MICROSCOPY

MICHAEL HOOKER MICROSCOPY
• Core Facility—open to all UNC investigators
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Confocal – fluorescence/reflection/frap/fret
Atomic Force Microscope
Laser Micro-Dissection
Light Microscopy –
tans./fluor./DIC/phase/multimode/time-lapse
http://mhmicroscopy.med.unc.edu
Mike Chua
843-3268
MICROSCOPY SERVICES
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UNC Core facility
Confocal
Laser Capture Microscopy
Electron Microscopy
Light Microscopy
http://www.med.unc.edu/microscopy/
Bob Bagnell 966-2413
FLOW CYTOMETRY CORE
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UNC Core Facility
Deconvolution System (3D)
Laser Scanning Cytometer
FACS
http://flowcytometry.med.unc.edu/
Larry Arnold 966-1530
R. SUPERFINE - T. SALMON LABS
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Scanning Electron Microscopy
Transmitted Electron Microscopy
Atomic Force Microscopy
3D Force Microscopy
Confocal Microscopy
Light Microscopy
[email protected]
[email protected]
RUSSELL TAYLOR- VISUALIZATION
LAB
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Scientific Visualization
Distributed Virtual Worlds
Haptic Display
Interactive 3D Computer Graphics
UNC NIH National Research Resources
for Computer Integrated Systems for
Microscopy and Manipulation-http://www.cs.unc.edu/Research/nano/cis
mm/
UNC NEUROIMAGING LABORATORY
G. Gerig, A. Belger, J. Lieberman, J. Piven
• Cognitive Neuroscience
– ERP lab: Functional brain mapping
– Functional brain mapping using fMRI
• Analysis of MRI diffusion tensor imaging (DTI)
• 3D ultrasound: Quantitative image analysis
• Developmental Neuroimaging: UNC-NDRC Core
• High-power computer system dedicated to neuroimage
analysis
Neurolab: http://zeus.ia.unc.edu/
NIRL: http://www.nirl.unc.edu/
NDRC: http://www.fpg.unc.edu/~ndrc/cores.cfm
BARRY WHITSEL LAB
• Intrinsic Optical Signal Imager (cell-based,
in vivo)
[email protected]
BIOLOGY
Kerry Bloom – Ted Salmon – Tony Perdue
• Spinning disk Confocals
• Laser Scanning Confocals
• Transmission Electron Microscopy
[email protected]
[email protected]
[email protected]
BIOMEDICAL ENGINEERING
• MRS Metabolomics Imaging (J. MacDonald)
• Cortical Intrinsic Signal Imaging
• Multiphoton fluorescence Imaging
• Transmembrane Potential Imaging
• Intracellular Calcium Imaging
• Biophotonics Computer Imaging
[email protected]
CELL & DEVELOPMENTAL BIOLOGY
J. LeMasters – J. Costello – K. Jacobson
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Confocal – Single & 2 Photon
Cryo – Transmitted Electron Microscopy
Scanning Electron Microscopy
Optical Tweezers
Fluorescence Recovery After Photobleaching
[email protected]
[email protected]
[email protected]
CHEMISTRY
• Nuclear Magnetic Resonance
• Spectroscopy
• Total Internal Reflection Microscopy –
Nancy Thompson
[email protected]
DENTAL SCHOOL
• Transmitted Electron Microscopy
• Scanning Electron Microscopy
Wallace Ambrose
[email protected]
PEDIATRICS/EPA—J. CARSON
• Freeze Fracture
• Transmitted Electron Microscopy
• Scanning Electron Microscopy
• Light Microscopy
[email protected]
RADIATION ONCOLOGY
Edward Chaney
• CT
• Segmentation
• Registration
• 3D & 4D Display
[email protected]
RADIOLOGY – RESEARCH
Weili Lin– Etta Pisano – Stephen Aylward
• 3 Tesla Magnetic Resonance Imaging (Lin)
• Micro Single-Photon Computed Tomography
(Lin)
• Diffraction Enhanced Imaging(Pisano)
• Computer Aided Diagnosis and Display Lab
(CADDLab: Aylward)
-- 2D and 3D image segmentation and registration
--Multi-modal image resgistration
[email protected]; [email protected];
[email protected]
COMPUTER-ASSISTED SURGERY
& IMAGING LAB (CASILAB)
Elizabeth Bullitt
• Vessel/Tumor
Imaging and Analysis
• Imaged Guided
Surgery
[email protected]
GENE THERAPY CENTER
• Optical Imaging (Cryocooled,
luminescence and fluorescence)
Allison Hawke
[email protected]
LINEBERGER COMPREHENSIVE
CANCER CENTER
• Light Microscopy
• Fluorescence Microscopy
Steve Oglesbee
[email protected]
NEUROSCIENCES & NEURODEVELOPMENTAL
DISORDERS RESEARCH CENTERS
Eva Anton - Robert Sealock
• Confocals – Single & 2-Photon
• Light Microscopy
[email protected]
[email protected]
VASCULAR BIOLOGY CENTER
Cam Patterson
• Ultrasound for small animals
[email protected]
MEDICAL IMAGE DISPLAY & ANALYSIS
GROUP (MIDAG)
• At Comp Sci, Psychiatry, Rad Onc, Radiology,
Surgery
• Analysis Capabilities
• Image Analysis
• Image Display
• Augmented Reality (Computer Science)
• Segmentation
• Registration
http://midag.cs.unc.edu
NC STATE UNIVERSITY
• BOTANY DEPARTMENT – Light Microscopy,
Confocal, Nina Allen, Box 7612, NCSU
• John MacKenzie – Coordinator, EM Center
[email protected]
• Phillip Russell – Dir, Analytical Instru. Facility
Atomic Force & TEM, [email protected]
• Michael Dykstra – Dir, Laboratory Advanced
Microscopy, TEM & Confocal
[email protected]
• VET SCHOOL – X-ray, CT, ultrasound, MRI
Ian Robertson, [email protected]
DUKE-UNC BRAIN IMAGING &
ANALYSIS CENTER (BIAC)
• fMRI Neuroscience acquisition and
analyses
http://www.biac.duke.edu/
POSSIBLE APPLICATIONS
(from NIH proposal – T. Van Dyke & B. Tsui)
High-resolution pinhole SPECT studies:
• Can do a series of images with a single
injection depending upon the length of the
half-life of the radionucleotide.
• Locating tumors and bone metastases
microPET studies:
• Locating a large variety of primary tumors
and their metastases
POSSIBLE APPLICATIONS
(from NIH proposal – T. Van Dyke & B. Tsui)
MRI and MRS studies:
• Tumor growth
• Changes in tumor vascularity
Optical Imaging studies:
• Luciferase localization
• Reporter gene expression
MicroCT studies:
• Tumor growth
FUTURE DEVELOPMENTS
(from NIH proposal – T. Van Dyke & B. Tsui)
• Use Animal Model systems for refining specific
imaging technologies—i.e. technologies to
measure blood flow and microvascular
permeability within tumors.
• Imaging results compared to histologic analyses
can help develop technologies to measure
disease progression and then be used to screen
development of disease in backgrounds with
additional genetic alterations.