Transcript Rate of diffusion of ethylene blue and red saffranin dye

Rate of diffusion of
methylene blue and red saffranin
dye
under three physical conditions
Project Three
SATURDAY SCIENCE ACADEMY
Group Members
 Brandon
Bell
 Alicia Blakley
 Isis Breadshaw
 Deitra Clark
 Cheryl Smith
 Melvin Stewart
Objective
To learn about the affect of temperature
on the diffusion of dye molecules in a
liquid medium. Heat energy excites molecules
and increases the kinetics of molecular
motion. In this exercise participants used ice-
cold water, boiling water and normal tap
water and recorded the time it took for the
complete diffusion of the dye molecules in
water under the above physical conditions..
Method


Two dye; saffranin and
methylene blue were
used in the experiment.
100 ul of the dye were
gently dropped in three
100 ml transparent
plastic cup containing
ice water, boiling water
and tap water.
Method
(Continued)

Time taken for equal
distribution of the dye
molecules across the
medium was recorded
for each observation.
Data
Dye
Methylene
Methylene
Methylene
Methylene
Methylene
Methylene
Methylene
Methylene
Methylene
Saffranin
Saffranin
Saffranin
Saffranin
Saffranin
Saffranin
Saffranin
Saffranin
Saffranin
Reps
Blue
Blue
Blue
Blue
Blue
Blue
Blue
Blue
Blue
Types
Initial
Time
Final
Time
Total
Time
Temp
1
Tap Water
10:58
11:46
48.0
25
2
Tap Water
11:09
11:51
42.0
24
3
Tap Water
11:10
11:57
47.0
25
1 Boiling Water
11:00
11:05
5.0
62
2 Boiling Water
11:06
11:07:30
1.5
75
3 Boiling Water
11:08
11:09:30
1.5
82
1
Ice Water
11:00
12:34
94.0
10
2
Ice Water
11:00
12:44
104.0
8
3
Ice Water
11:00
12:46
106.0
8
1
Tap Water
11:00
11:43
43.0
24
2
Tap Water
11:00
11:50
50.0
25
3
Tap Water
11:00
11:56
56.0
23
1 Boiling Water
11:00
11:08
8.0
65
2 Boiling Water
11:15
11:20
5.0
73
3 Boiling Water
11:25
11:31
6.0
72
1
Ice Water
11:05
11:30
25.0
8
2
Ice Water
11:06
11:40
34.0
6
3
Ice Water
11:00
11:35
35.0
8
Data (Continued)
Methylene Blue Saffranin
Tap Water
Tap Water
Tap Water
Boiling Water
Boiling Water
Boiling Water
Ice Water
Ice Water
Ice Water
48.0
42.0
47.0
5.0
1.5
1.5
94.0
104.0
106.0
43.0
50.0
56.0
8.0
5.0
6.0
25.0
34.0
35.0
Average Time
Methylene Blue Saffranin
Tap Water
Boiling Water
Ice Water
45.7
2.7
101.3
49.7
6.3
31.3
Standard Deviation
Methylene Blue Saffranin
Tap Water
Boiling Water
Ice Water
3.2
2.0
6.4
6.5
1.5
5.5
The collected data were subjected
to descriptive statistics on data.
Rate of Diffusion
120.0
100.0
80.0
Tap Water
Time in
60.0
Minutes
40.0
Boiling Water
Ice Water
20.0
0.0
Methylene Blue
Satranin
Regress Time of diffusion of
methylene blue dye on Temperature
Temp Line Fit Plot
150.0
Total Time
Regression Statistics
Multiple R
0.924
R Square
0.854
Adjusted R Square
0.834
Standard Error
17.544
Observations
9
100.0
Total Time
50.0
Predicted Total
Time
0.0
-50.0
0
50
Temp
Intercept
Temp
Coefficients Standard Error
97.722
9.482
-1.350
0.211
t Stat
P-value
10.306 1.75376E-05
-6.408 0.000364427
100
Conclusion
 The
Dye methylen blue responded
significantly in the three physical
conditions.
 Rate of diffusion was the least at ice
water.
 Fastest diffusion was observed in hot
water.