Transcript Consumer Products Study - Montclair State University

Consumer Products Study
Disinfectants, Mouthwash
Antibacterial Hand Soap,
Antibiotics, and Spices
By THE WESTON SCHOLARS!!!!!
(and gifted and talented students.)
MATERIALS
 Tape
 Wax pencil
 Petri dish
 Cotton swabs
 Forceps
 Nutrient broth
 Sterile disk
 Inoculating wire loop
 Sterile water
 Bunsen burner
 Nutrient agar plate
 All consumer products
Procedure
Preparing Nutrient Agar Plates
1. Suspend 23g of nutrient agar powder in 1L
purified H2O.
2. Mix and heat solution until powder is dissolved
and solution becomes an translucent yellow.
3. Autoclave at 121ºC for 15 minutes.
4. Fill as many petri dishes as possible (half full)
with nutrient agar and let them solidify.
Procedure (cont’d)
5. Dip cotton swab in sterile H2O and take
samples from mouth, skin, and
environment.
6. Rub the swab on nutrient agar plate.
7. Cover nutrient agar plates and label them.
8. Invert nutrient agar plates and place them
into the incubator at 37ºC.
Procedure (cont’d)
9. For discontinuous streaking, sterilize wire
loop by flaming until the wire turns bright
orange.
10.Cool loop by lightly touching the wire to the
nutrient agar.
11.Using the wire loop, take bacteria from the
nutrient agar plate and streak 4 times on a
new agar plate and flame the loop in
between.
12.Cover, label, and tape plates shut.
13.Place, inverted, in the incubator at 37°C.
Discontinuous Streaking
Procedure (cont’d)
14. For the continuous streaking, use the same
procedure as the discontinuous streaking,
except you just streak back and forth until
you cover the whole nutrient agar plate.
Continuous Streaking
Procedure (cont’d)
Re-suspend bacteria
1. Pour 10ml of nutrient broth into the continuously streaked
plates.
2. Swab bacteria until all bacteria is suspended in liquid
3. Swab bacteria into new plates.
4. Dip sterile disk in disinfectants, antibiotics, spices,
mouthwash, or antibacterial hand soap and place in center of
plates. Tap gently to secure.
5. Label plates and place them inverted in the incubator.
6. Let plates sit in the incubator over night.
7. Measure the zone of inhibition (in millimeters) and make
your conclusions.
Zone of Inhibition
Disinfectants
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Ajax
Algacide
America’s Choice Pine Cleaner
Ammonia
10% Bleach
Clorox
Fantastik-Clear
Fantastik-Purple
Scrubbin’ Bubbles
Soft Scrub
Spic and Span
Tilex
Touch of Glass
Wavicide
White Vinegar
Windex
Xtra Pine
409
Disinfectants
Results
Disinfectants
Zone of Inhibition
Ajax
25mm
Algacide
19mm
America’s Choice
9mm
Clear Ammonia
0mm
Clorox
30mm
Clear Fantastik
11mm
Purple Fantastik
14mm
Scrubbin’ Bubbles
10mm
Soft Scrub
9mm
Spic and Span
12mm
Tilex
9mm
Touch of Glass
1mm
Wavicide
5mm
White Vinegar
15mm
Windex
1mm
Xtra Pine
10mm
409
23mm
10% Bleach
0mm
Results
Disinfectants Zones of Inhibition
35
Zone of Inhibition (mm)
25
20
25
Scrubbin Bubbles
Soft Scrub
Spic and Span
19
15
14
11
9
10
Tilex
12
9
Touch of Glass
10
9
Wavicide
White Vinegar
5
5
0
Fantastik-Clear
Fantastik-Purple
23
15
10
Americas Choice
Clear Ammonia
Clorox
30
30
Ajax
Algacide
1
0
Disinfectants
1
Windex
0
Xtra Pine
Four o' Nine
Ten Percent Bleach
Best Disinfectants
#1
#3
#2
Mouthwashes
•
•
•
•
•
•
•
•
•
•
•
•
Biotene
Cepacol
CVS Brand
Diluted Hydrogen Peroxide
Listerine
Listerine Tartar Control
Plax
Rite Aid Brand
Salt Water
Scope
ShopRite Brand
Tom’s Natural Mouthwash
Results
Mouthwashes
Zone of Inhibition
Biotene
2mm
Cepacol
12mm
CVS Brand
3mm
Hydrogen Peroxide
18mm
Listerine
2mm
Listerine Tartar Control
2mm
Plax
10mm
Rite Aid Brand
3mm
Salt Water
0mm
Scope
3mm
ShopRite Brand
3mm
Tom’s Natural Mouthwash
0mm
Results
Mouthwash's Zones of Inhibition
20
Zone of Inhibtion (mm)
DILUTED HYDROGEN
PEROXIDE
LISTERINE
16
14
12
12
LISTERINE TARTAR
CONTROL
PLAX
10
10
8
RITE AID
6
4
2
CEPACOL
CVS
18
18
BIOTENE
2
3
2
3
2
3
SALT W ATER
3
SCOPE
0
0
1
Mouthwashes
0
SHOPRITE
TOMS NATURAL
MOUTHW ASH
Best Mouthwashes
#3
#1
#2
Hand Soaps
•
•
•
•
•
•
Bath & Body Works
Clean n Smooth
Ajax
Palmolive
Soft Soap
April Body & Shower
Results
Type of Soap
Zone of Inhibition
Clean & Smooth
4mm
Bodyworks
1mm
Softsoap
17mm
Palmolive
15mm
April Bath & Shower
3mm
AJAX
18mm
Results
S
A
pr
il
B
a
th
&
P
Name of Soap
X
JA
A
ho
w
er
ol
iv
e
al
m
oa
p
S
of
ts
ks
od
yw
or
B
an
&
S
m
o
ot
h
19.00
18.00
17.00
16.00
15.00
14.00
13.00
12.00
11.00
10.00
9.00
8.00
7.00
6.00
5.00
4.00
3.00
2.00
1.00
-
C
le
Zone of Inhibition
Soaps
Best Hand Soaps
#3
#1
#2
Antibiotics on the skin bacteria
•
•
•
•
•
•
•
•
•
Tetracycline
Rifampin
Chloramphenicol
Kanamycin
Streptomycin
Triple Sulfonides
Polymyxin B
Erythomycin
Cephalothin
Results
Name of Antibiotics Zone of Inhibition
Sig. of Zone of Inhibition
Tetracycline
21mm
Sensitive
Cephalothin
25mm
Sensitive
Rifampin
28mm
Sensitive
Chloramphenicol
23mm
Sensitive
Kanamycin
18mm
Sensitive
Streptomycin
12mm
Intermediate
Triple Sulfonides
0mm
Resistant
Polymyxin B
10mm
Intermediate
Erythomycin
15mm
Resistant
Te
tra
cy
c li
ne
Ce
ph
alo
thi
n
Ri
fa
Ch
m
lor
pin
am
ph
en
ic o
Ka
l
na
m
yc
St
in
re
pt
om
Tr
yc
ipl
in
e
Su
lf o
nid
es
Po
lym
yx
in
Er
B
yth
om
yc
in
Zone of Inhibition
Results
Antibiotics
30.00
25.00
20.00
15.00
10.00
5.00
0.00
Name of Antibiotics
Best Antibiotics
#1 Rifampin
#2 Caphalothin
#3 Chloramphenicol
Spices
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Cinnamon
Paprika
Chili Powder
Onion Powder
Cream of Tartar
Coriander
Mustard Powder
All Spice
Alum
Cumin
Nutmeg
Cajun Seasoning
Curry
Ground Ginger
Garam Marsala
Garlic
Results
Spices
Zone of Inhibition
Cinnamon
5mm
Paprika
0mm
Chili Powder
3mm
Onion Powder
2mm
Cream of Tartar
9mm
Coriander
0mm
Mustard Powder
4mm
Allspice
6mm
Alum
10mm
Cumin
4mm
Nutmeg
4mm
Cajun Seasoning
2mm
Curry
3mm
Ground Ginger
2mm
Garam Masala
4mm
Garlic
10mm
Results
Spices as Disinfectants
12
10
Zone of Inhibition (MM)
10
10
9
8
6
6
5
4
4
4 4
4
3
3
2
2
2
0
0
0
Spices
2
Cinnamon
Paprika
Chili Powder
Onion Powder
Cream of Tartar
Coriander
Mustard Powder
All Spice
Alum
Cumin
Nutmeg
Cajun Seasoning
Curry
Ground Ginger
Garam Marsala
Garlic
Best Spices
#3
#1
#2
Thank you!
Dr. Lee H. Lee
Pat Platner
Ms. English
Shi-Fang Hsu
Ms. Portas
Tina Chu
Alex Peroff
AND
Mr. & Mrs. Weston