Transcript Autoclave Training - EHS Director's Roundtable

Autoclave Training
November 16, 2005
University of Kentucky
Department of Biosafety
Learning Objectives
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What materials can be autoclaved?
What Personal Protective Equipment (PPE) is
necessary for autoclave use?
How does one properly load the autoclave?
What steps assist in efficient decontamination
of biohazardous waste?
How does one verify autoclave performance
for decontamination of biohazardous waste?
Importance of Proper Autoclave Use
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Tremendous pressure from steam in chamber
provides explosive potential
High temperatures and presence of extremely hot
water creates potential for burns and scalding
Inadequate decontamination allows for the potential
of biological hazards and personnel and
environmental contamination
Autoclave Explosion
What can be autoclaved
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Surgical Instruments
Glassware
Plastic tubes and pipette tips
Solutions and water
Animal food and bedding
Waste
Types of waste
Waste Type
Container
What You Need To Do
Biological Infectious Waste
Orange or clear autoclave bag
Autoclave
Medical Waste
Biological Infectious Waste which
cannot be autoclaved
Red incineration bag in red waste
container
Medical Center- close and place
by trash in lab for pick-up,
Outside Medical Center- contact
Environmental Management for
pick-up
Sharps- All needles, syringes,
scalpels, blades, and all other
sharp medical waste
Sharps container
Medical Center- close and place
by trash in lab for pick-up,
Outside Medical Center- contact
Environmental Management for
pick-up
Animal Carcasses- no biological,
chemical, or radiological hazard
Opaque bag
Return to appropriate DLAR
location
Animal Carcasses- intentionally
infected
Red incineration bag
Incinerate (Medical Center or call
Environmental Management pickup)
Used Biological Culture Media
Glass or plastic
Disinfect with bleach or autoclave
Principles of Autoclave Operation
• Steam penetrates objects in the autoclave
• Condensation creates negative pressure and
draws in additional steam
• Moist heat kills microorganisms via
coagulation of proteins
• Two types of autoclaves
• Gravity Displacement
• Vacuum Assisted
Gravity Displacement
Vacuum Assisted
Closed
Closed
Air
Air
Air
Air
Open
Open
Open
Open
Steam
Steam
Steam
Steam
Closed
Closed
Autoclave Use Basics
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Personal Protective Equipment (PPE)
Packaging
Loading
Operating
User logs
Maintenance logs
Unloading
Improper Autoclave Practices
PPE for Autoclave Users
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Eye Protection
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Lab Coat, Buttoned
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Closed-toed Shoes
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Heat-resistant Gloves
Packaging for Autoclaving
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Be sure the material should be autoclaved
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Utilize containers and autoclave bags
appropriate for autoclaving
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No volatile chemicals, sharps, red bag waste,
radioactivity
Clear or orange bags
UK Stores 6532-0046
Do not overfill containers
Packaging for Autoclaving
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Carefully add water to autoclave bags
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Prepare packaging to allow steam penetration
Check that all containers including bags are vented
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Add approximately 1 liter of water to 24” x 30” bag
Aids in reaching appropriate temperature quickly
Creates steam in the autoclave bag to replace cold air
Loosely close autoclave bags
Vent lids on bottles containing solutions
Place packaged material in a secondary container
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Stainless steel tray or autoclavable polypropylene bin
Loading the Autoclave
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Load material to allow efficient steam
penetration
Autoclave clean items and waste separately
Do not allow material to be autoclaved to
touch the sides or top of the chamber
Operating the Autoclave
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Be sure the autoclave is functioning properly before use
Record information in User Log
Close door properly and securely
Choose the correct conditions for your material
For decontamination:
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Temperatures between 121-124°C
Total processing time 60-120 minutes
Exposure time greater than 20 minutes
Pressure minimum 15 PSI
Select liquid cycle or slow exhaust cycle
Autoclave User Log
• Maintain User Logs for Two Years
Date
Time
EX 1/1/2005 10:23 AM JD
1
2
3
4
5
6
2
Biohazard Waste
Initials
Lab Biosafety
Level (BSL 1, 2, or
3)
Log Number
• Complete User Log with Every Use
Time Temp
Lab Room #
Media Other (min.) . (°C)
EHS 123
X
60
121°C
Pressure
(PSI)
15
Tape strip here. If autoclave
generated documentation, please
Results
initial to indicate that waste load
(Pass/Fail)
met parameters.
pass
CITP strip tapped here showing pass
Autoclave Maintenance Log
• Maintain Maintenance Logs for Five Years
• Complete Maintenance Log for Every Repair
• Maintain records of Yearly Calibration
AUTOCLAVE MAINTENANCE LOG (to be kept with copy of Instruction Manual at each Autoclave)
Building/Room:
_____________
Serial Number:
_______________
Model:
_____________
Contact Person: ________________________
Date
Problem
Remedial Action/Operator Initials
Service Description
Comments
Make: ________________
Unloading the Autoclave
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Put on Personal Protective Equipment
Allow the autoclave to completely finish cycle
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Pressure gauge must read zero
Verify cycle conditions were met
Open door slightly to allow steam to escape
Allow contents to cool before removal
Carefully remove items
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Be especially careful with fluids and plastic bins
Improper Autoclave Practices
Results of Improper Autoclave Use
Autoclave Verification Program
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Program will ensure that all potentially
hazardous biological waste is properly
decontaminated prior to deposition in landfill
Program requires testing of the ability of
campus autoclaves to kill microorganisms
There are over 50 autoclaves on campus
which require this verification program
Autoclave Verification
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Each Load of Biohazardous Waste
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Autoclaves with automatic documentation
 Review the printed report
 If conditions were met, initial, date, and place User Log
number on printed report
 If conditions were not met, do not remove the load, repeat
autoclave cycle.
 If conditions are not met again, autoclave must be labeled
out of order.
 Contact responsible individual posted on autoclave.
 Responsible individual contacts service company to
schedule repairs.
Autoclave Verification
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Each Load of Biohazardous Waste
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Autoclaves without automatic documentation
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A Chemical Integrator Test Pack (CITP) must be
incorporated in each load.
Based on color change the performance of the
autoclave will be evaluated.
Tape the autoclaved CITP strip in the appropriate
section of the User Log for your load.
Autoclave Verification
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Monthly Verification for Biological Waste
Autoclaves
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Biological Indicator Test
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Checks all conditions of autoclave cycle, time,
temperature, and pressure
Verifies ability of autoclave to kill a microorganism
Utilize the 3M Attest Biological Indicator Monitoring Kit
Record results in Biological Indicator Results Log
Biological Indicator Results Log
• Maintain Results Logs for Five Years
• Complete Results Log with Every Monthly Test
Biological Indicator Test Results Log (Monthly)
Building/Room#:
Room E-Bar Number:
#:
Model:__________
Serial
Biological Indicator Type and Brand: ____________________________ Contact
Person/Department:______________________
Operator Name
Date of
Autoclaving
and
Incubation
Cycle Selected and
Temperature
Cycle
Time
Incubation
Time
(Hours)
Biological Indicator Lot
Number and Expiration
Date
Results
Pass
Fail
Biological Indicator Test Procedure
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Label Attest package containing biological
indicator vial with date, autoclave ID, and load
number from user log
Place the indicator vial package in the autoclave
with the biohazard waste load
Run the autoclave cycle as usual
Using proper unloading procedures, remove the
indicator package from the autoclave
Biological Indicator Test Procedure
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Label the indicator vial with all information from
package
Check indicator vial label for color change from rose to
brown
Place the biological indicator vial into the 56°C
incubator
Biological Indicator Test Procedure
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Place a non-autoclaved, labeled control tube
in the incubator
Examine the indicator tube at 8, 12, 24, and
48 hours for any color change
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Yellow color change indicates bacterial growth
Compare to non-autoclaved control at each time
point
Record 48 hour test results in Results Log
Biological Indicator Test Results
Unprocessed
Positive Control
Processed
Learning Objectives Review
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What materials can be autoclaved?
Surgical Instruments
Glassware
Plastic tubes and pipette tips
Solutions and water
Animal food and bedding
Waste
Learning Objectives Review

What Personal Protective Equipment (PPE) is
necessary for autoclave use?
Learning Objectives Review

How does one properly load the autoclave?
Load material to allow efficient steam penetration
Autoclave clean items and waste separately
Do not allow material to be autoclaved to touch
the sides or top of the chamber
Learning Objectives Review

What steps assist in efficient decontamination
of biohazardous waste?
Add approximately 1 liter of water to 24” x 30” bag
Loosely close autoclave bags
Place packaged material in an approved 2° container
Learning Objectives Review
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How does one verify autoclave performance for
decontamination of biohazardous waste?
Each Load
Verify cycle conditions via autoclave printout
CITP indicator use
Monthly
Utilize Attest Biological Indicator Vial
References and Links
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http://ehs.uky.edu/biosafety/autoclave.html
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http://www.uottawa.ca/services/ehss/autoclaves.htm
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http://www.3m.com/
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http://www2.umdnj.edu/eohssweb/aiha/technical/labequipment.
htm#Autoclave
http://ehs.uky.edu/em/ Environmental Management 323-6280
Questions or Additional Information
Brandy Nelson
Sr. Biological Safety Specialist
Department of Biological Safety
252 East Maxwell Street
Lexington, KY 40506-0314
Phone: 859-257-3753
Cell: 859-699-6082
[email protected]
http://ehs.uky.edu