Transcript ABO & Rh Blood Typing Lab - Mrs. Basepayne's Science Spot

ABO & Rh Blood Typing Lab
1. Name in MLA Format
2. Title
3. Read the introduction and write a 5 sentence introduction
summary for the lab.
4. Hypothesis – based on your family genetics what blood type
do you think you are?
5. Read and write down the procedures so you know what to do
Wednesday
6. Draw Tables 1 and 2. For table 1 increase the tables to
include 4 blood samples (1 for each person in your group)
7. Write down the following assessment questions leaving room
to answer each question:
1.
1 – 9, 11-12
8. Conclusion – be sure to address whether your hypothesis was
right or wrong, what you learned, etc.
Lab Write Up Procedures
1. Put on all lab safety equipment.
2. Wash your hands with soap and water.
3. Thoroughly clean the tip of one finger on your non-writing
hand with a sterile alcohol pad.
4. Carefully remove a lancet package from the end that is closest
to the blunt end of the lancet and then remove it.
5. Prick the sterile area on your finger with the lancet.
• If you cannot perform this step, ask your teacher for help.
6. Carefully place the lancet back in its package and dispose of it
in the biohazard bag.
Procedures
7. Add one drop of blood to each well of the blood typing
tray.
• Make sure that you work quickly and there is enough
blood that it does not dry to the tray.
8. Clean the tip of your finger with a new sterile alcohol
pad and dispose of it in the biohazard bag.
9. Add one drop of anti-A serum to the A well, one drop of
the anti-B serum to the B well, and one drop of anti-Rh
to the Rh well.
10.Using a clean toothpick, stir the A well thoroughly.
Dispose of the toothpick in the biohazard bag.
11. Repeat step 10 for B and Rh wells. Be sure to use a new
toothpick for each well to avoid cross contamination.
12. Examine each well for agglutination. Agglutination indicates a
positive test result.
13. Record your results in Table 1 and determine your blood type.
14. Pool the class data and calculate the percentage of students with
each blood type using the following formulas:
Total number of students with type (x) blood X 100
Total number of students in class
15. Record your results in Table 2.