Transcript A new medium for isolators and cleanrooms

New Product Anouncement
Cephalosporinase - Plates
Requirements for Plates !
• Inactivation of a broad
spectrum of cephalosporins
• Inactivation of residuals of
disinfectants (especially
contact plates)
ß-Lactam-Antibiotics
•
Penicillins
•
1st Generation Cephalosporins:
•
2nd Generation Cephalosporins:
•
3rd Generation Cephalosporins:
•
•
4th Generation Cephalosporins:
Cefquinom, Cefepim
•
Carbapenems:
Ampicillin, Mezlocillin, Penicillin
Cefazolin, Cefalotin, Cefalexim
Cefamandol, Cefuroxim, Cefoxitin (Cefomycin or Methoxa-Cephalosporine)
Cefotaxim, Ceftriaxon (Rocephin), Ceftazidim
Meropenem, Imipenem, Ertapenem
Activity of ß-Lactamase I
• ß-Lactamase I (= Penicillinase or Penase)
hydrolyses the ß-Lactam ring of Penicillins
• Definition - Activity of Penicillinase:
1 Unit = Cleavage of 1 Micromol Penicillin G
per minute at pH 7 and 25°C
Activity of ß-Lactamase II
• ß-Lactamase II (= Cephalosporinase or
Cephase) hydrolyses the ß-lactam ring of
Cephalosporins
• Definition - Activity of ß-Lactamase II:
1 Unit = Cleavage of 1 Micromol
Cephalosporin C per minute at pH 7 and
25°C
Activity of different ß-Lactamase II
Media - Tests using antibiotic discs Inactivation of different Cephalosporins
(test strain S. aureus ATCC 6538 / (antibiotic discs)
TSA-Contact +LTHTh+ßLactamaseII - ICR (2281e)
TSA Contact +LT +ßLactamaseII - ICR+ (825)
Biomerieux 3P Contact TSA+Neutr.+ßLact.
49
43
44
diameter of inhibition zone (mm)
39
34
34
31
30
28
29
25
24
24
23
24
21
20
19
25
18
19
14
13
14
13
12
14
10
10
9
7
5
4
0
0
0
0
0
0
0
Cefazolin 30
Meropenem 10
Cephotaxim 30
Ceftriaxon 30
Imipenem 10
-1
Cefexim 5
Cefuroxim 30
Cefquinom 10
Cefepim 30
Cefoxitin 30
Activity of different ß-Lactamase II
Media - Tests using anitbiotic discs Inactivation of different Cephalosporins
(test strain S. aureus ATCC 6538 / (antibiotic discs)
TSA+ß-Lactamase II -ICR (03079e)
TSA+LT+ßLactamaseII -ICR (03080e)
Biomerieux 3P Sedimentation TSA+Neutr.+ßLact.
44
41
diameter of inhibition zone (mm)
39
33
34
29
27
24
26
34
27
23
22
19 19 19
19
17
14
14
14
13
16
15
16
13 13
11
9
4
0
0
0
0
0
0
0 0
0
-1
Cefexim 5
Cefazolin 30
Meropenem 10
Cephotaxim 30
Ceftriaxon 30
Imipenem 10
Cefuroxim 30
Cefquinom 10
Cefepim 30
Cefoxitin 30
Summary of spectrum of efficacy for TSA
Contact +LT +ß-Lactamase II
(Article number 825)
• Good inactivation in antibiotic disc experiments:
– Cefixim 5 µg /disc
– Cefazolin 30 µg /disc
– Meropenem 10 µg /disc
– Cefotaxim 30 µg /disc
– Ceftriaxon 30 µg /disc
– Imipenem 10 µg /disc
•
Moderate inactivation in antibiotic disc experiments:
– Cefuroxim 30 µg /disc
– Cefquinom 10 µg /disc
• Poor or missing inactivation in antibiotic disc experiments:
– Cefepim 30 µg /disc
– Cefoxitin 30 µg /disc
Developement of new
Cephalosporinase with a broad
inactivation spectrum
LACTAMATORTM
Inactivating Properties of Different ßLactamase II Media - Tests using
Antibiotic Discs and S. aureus Method:
1. Inoculation of >106 CFU of S. aureus ATCC 6538 - 2. Antibiotic disc is applied - 3. Incubation for 1d at 34°C - 4. Determination of inhibition zone
(diameter in mm)
46
47
TSA Contact + LTHTh - ICR+
(article 820, without
Cephalosporinase)
40
41
38
36
34
Inhibition diameter (mm)
35
31
29
28
29
28
27
25
25
31
29
32
TSA Contact + LTHTh + ßLactamase II - ICR (article
2281e)
2727
27
24
21
23
32
21
21
18
16
17
12
11
TSA Contact + LT + ßLactamase II - ICR+ (article
825)
11
11
TSA Contact + LT +
Cephase - ICR+ (article 823)
5
0
0
00
00
0
00
00
0
0
00 0
00 0
00 0
0 0
C
C
ef
ep
im
(3
ef
0
qu
µg
in
)
om
C
(
10
ef
ot
µg
ax
)
im
C
(
30
ef
tri
µg
ax
)
on
C
(
3
ef
0
ur
µg
ox
)
im
(
Im
30
ip
µg
en
)
e
m
M
er
(1
op
0
µg
en
)
em
Er
(1
ta
0
pe
µg
ne
)
m
(
C
10
ef
µg
ox
)
iti
n
(
3
Pe
0
µg
ni
ci
)
ll in
(1
Am
0
pi
µg
ci
)
ll in
M
(
2
ez
5
µg
lo
zi
)
l li
n
C
(3
ef
0
az
µg
ol
)
in
(3
0
µg
)
-1
Inactivating Properties of Different ßLactamase II Media - Tests using
Antibiotic Discs and K. rhizophila Method:
1. Inoculation of >106 CFU of K. rhizophila ATCC 9341 - 2. Antibiotic disc is applied - 3. Incubation for 1d at 34°C - 4. Determination of
inhibition zone (diameter in mm)
47
45
44
41
39
39
41 38
40
40
38
36
Inhibition diameter (mm)
35
34
35
TSA Contact + LTHTh - ICR+
(article 820, without
Cephalosporinase)
42
35
34
33
32
31
30
29
TSA Contact + LTHTh +ßLactamase II - ICR (article
2281e)
31
30
29
29
26
25
23
19
17
14
14
12
12
14
12
13
TSA Contact + LT + ßLactamase II - ICR+ (article
825)
11
TSA Contact + LT +
Cephase - ICR+ (article 823)
5
0
0
0
0
0
0
00
0
00 0
0 00
00 0
C
C
ef
ep
im
(3
ef
0
qu
µg
in
)
om
C
(
1
ef
0
ot
µg
ax
)
im
C
(3
ef
0
tri
µg
ax
)
on
C
(
30
ef
ur
µg
ox
)
im
(
Im
30
ip
µg
en
)
e
m
M
er
(1
op
0
µg
en
)
em
Er
(
ta
10
pe
µg
ne
)
m
(1
C
0
ef
µg
ox
)
iti
n
(
30
Pe
µg
ni
ci
)
ll in
(
Am
10
pi
µg
ci
)
ll in
M
(
25
ez
lo
µg
zi
)
l li
n
C
(3
ef
0
az
µg
ol
)
in
(3
0
µg
)
-1
0
Summary of spectrum of efficacy for TSA
Contact +LT +Cephase ICR+
(Article number 823)
Good inactivation of the following antibiotics:
– Penicillins:
• Ampicillin 25 µg, Mezlocillin 30 µg, Penicillin 10 µg
– Cephalosporins 1st generation:
• Cefazolin 30 µg /disc
– Cephalosporins 2nd generation:
• Cefuroxim 30 µg, Cefoxitin 30 µg (Cephamycin)
– Cephalosporins 3rd generation:
• Cefotaxim 30 µg, Ceftriaxon 30 µg
– Cephalosporins 4th generation:
• Cefepim 30 µg, Cefquinom 10 µg
– Carbapenems:
• Ertapenem 10 µg, Imipenem, 10 µg, Meropenem 10 µg
New Products
•
TSA Contact +LT + Cephase – ICR+ - article number 823
Product available for advance orders
shelf life of 4 months completed in June 2009
•
TSA +LT + Cephase – ICR – article number 03085e
Product available for advance orders
shelf life of 4 months completed end of July 2009
•
•
LACTAMATORTM LCT-5
(10 vials each with 50 IU Cephalosporinase and 500 IU Penicillinase,
lyophilised)
Product available in April 2009
•
•
LACTAMATORTM LCT-20
(1 vial with 2 kIU Cephalosporinase and 20 kIU Penicillinase,
lyophilised)
Product available in April 2009