Transcript Бактериальная люцифераза

Bacterial luciferase
(from Vibrio harveyi)
α
субъединицы
Молекулярная
масса
α
40 кДа
Число
355
аминокислотных
остатков
β
heterodimer 77 кДа
EC#: 1.14.14.3
Structure Weight: 76965.65
β
37 кДа
324
Катализирумая реакция
+
+
RCHO
FMNH2
=
RCOOH
+
H
FMN
H
+
hv
478-505 нм
Luciferase surface
Fisher A J et al. J. Biol. Chem. 1996;271:21956-21968
©1996 by American Society for Biochemistry and Molecular Biology
Active site pocket surface map
Fisher A J et al. J. Biol. Chem. 1996;271:21956-21968
©1996 by American Society for Biochemistry and Molecular Biology
Crystal Structure of the Bacterial
Luciferase:Flavin Complex
The structure 3FGC has in total 4 chains. Out
of these 2 are sequence-unique.
http://www.proteopedia.org/wiki/index.php/3fgc
http://www.ebi.ac.uk/pdbsum/3fgc
Secondary structure
Structure Weight:
157490.22
http://www.rcsb.org/pdb/explore/explore.do?structureId=3FGC
http://www.rcsb.org/pdb/explore/jmol.do?struct
ureId=3FGC&bionumber=1
http://www.rcsb.org/pdb/explore/remediated
Sequence.do?structureId=3FGC
Источники:
1) The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions.
Fisher, A.J., Thompson, T.B., Thoden, J.B., Baldwin, T.O., Rayment, I. J. Biol. Chem.
1996;271:21956-21968
2) Crystal Structure of the Bacterial Luciferase:Flavin Complex Provides Insight into the
Function of the subunit, Campbell Z.T., Weichsel A, Montfort W.R., Baldwin T.O.,
Biochemistry. 2009 May 12.
http://www.rcsb.org/pdb/explore/jmol.do?structureId=3FGC&bionumber=1