Transcript Ahut_China - 2013.igem.org

Shining Purifier
Methods
1. Biological method
Ammonia nitrogen biological removal
2. Bio-chemical method
3. Physic-chemical method
a. Blow-off method
b. Membrane separation technique
c. MAP precipitation method
Anaerobic
ammonium
oxidation
(Anammox)
bacteria
L. van Niftrik et al., 2008. Combined structural and chemical analysis of the anammoxosome:
A membrane-bounded intracytoplasmic compartment in anammox bacteria.
Journal of Structural Biology 161 (2008) 401–410
NH4+ + NO2- → N2 + 2H2O
1.30
1.25
Mathematical model
CFU/ml
(Bacterial
Population)
A
1.20
ds
 rs (1  s / S m )
dt
1.15
1.10
1.05
Days
1
2
3
4
1.0
CFU/ml
(Bacterial
Population)
B
ds2
s2
s1
 r2 s 2 (1 
 2
)
dt
N2
N1
A
ds1
s1
s2
 r1 s1 (1 
 1
)
dt
N1
N2
0.8
0.6
0.4
Days
1
2
3
4
5
1.2
CFU/ml
(Bacterial
Population)
1.0
A
s3
ds1
s1
s2
 r1s1 (1 
 1
3
)
dt
N1
N2
N3
C
ds3
s
s
 r3 s3 (1  3   4 1 )
dt
N3
N1
B
ds2
s
s
 r2 s 2 (1  2   2 1 )
dt
N2
N1
0.8
0.6
0.4
Days
2
4
6
8
10
1.4
D
s
ds1
s
s
 r11 s1 (1  1   1 2   3 3 )
dt
N1
N2
N3
C
ds2
s2
s1
 r2 s 2 (1 
  21
)
dt
N2
N1
B
ds3
s3
s1
 r3 s3 (1 
  41
)
dt
N3
N1
CFU/ml
(Bacterial
Population)
1.2
1.0
0.8
0.6
0.4
2
4
6
8
Days
10
1.4
D
s3
ds1
s1
s2
 r11 s1 (1 
 1
3
)
dt
N1
N2
N3
A
s3
ds1
s1
s2
 r1 s1 (1 
 1
3
)
dt
N1
N2
N3
CFU/ml
(Bacterial
Population)
1.2
1.0
0.8
0.6
0.4
Days
2
4
6
8
10
Delft University of Technology, Department of Biotechnology,
Environmental Biotechnology Group
Key enzymes
NIR- Nitrite reductase
BBa_K1196018
HS- Hydrazine synthetase
BBa_K1196019
HAO- Hydrazine oxidoreductase
BBa_K1196020
Fdh- Formate dehydrogenase
BBa_K1196021
NADH- Ubiquinone reductase
(H+- translocating)
BBa_K1196022
Cyt bc1- Cytochrome C reductase BBa_K1196023
酶机理图 ?
devices
00910 7/12/13
(c) Kanehisa Laboratories
Delft University of Technology, Department of Biotechnology,
Environmental Biotechnology Group
Ladderane
lipids in the
anammox
membrane
Delft University of Technology, Department of Biotechnology,
Environmental Biotechnology Group
酶机理图 ?
devices
00910 7/12/13
(c) Kanehisa Laboratories
荧光细菌效果
Human Practice
Jian xu
School Tryouts
Partner
and
Sponsers
Sewage
treatment
New force
Propaganda
Survey
Findings
Acknowledgement
ANHUI UNIVERSITY OF
TECHNOLOGY
Thank you for listening!
Central catabolism of anammox bacteria
00910 7/12/13
(c) Kanehisa Laboratories
ANHUI UNIVERSITY OF
TECHNOLOGY
Sheng Ding
L. van Niftrik et al., 2008. Combined structural and chemical analysis of the anammoxosome:
A membrane-bounded intracytoplasmic compartment in anammox bacteria.
Journal of Structural Biology 161 (2008) 401–410
00910 7/12/13
(c) Kanehisa Laboratories
NH4+ + NO2- → N2 + 2H2O
酶机理图 ?
devices
00910 7/12/13
(c) Kanehisa Laboratories