Transcript 2. IRRI's Pedigree selection

Planning rice breeding programs
for impact
Pedigree Selection
in the IRRI Irrigated Breeding Program
Parminder S. Virk
PBGB Division
Can anyone define
“pedigree”?
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Learning objectives
•
Describe the pedigree method
•
Describe naming of pedigree at IRRI
•
Describe crosses at IRRI
•
Describe types of crosses
•
Demonstrate IRRI nurseries
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Pedigree Method
The pedigree method owes its name to the
pedigree record of selected plants maintained to
trace ancestral relationship among such plants
A detailed description of present day pedigree
method was outlined by Love (1927)
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Pedigree Method
In this method individual plants are selected starting with F2
and in the subsequent segregating generations their
progenies are tested till the progenies become homozygous.
During entire operation a record of all parent-offspring
relationship is kept: PEDIGREE RECORD
In this method each progeny in every generation can be
traced back to the original F2 plant
Pedigree describes parents, grandparents,
great-grandparents,...
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How is the naming of
pedigree at IRRI?
Based on cross number and serial numbers of
selected plants
IR numbers are assigned consecutively to the
crosses (now IR80389) made at IRRI just before
the hybrid seeds are incubated for germination
When plant selections are made from F2 and
subsequent generations of an IR cross, numerical
designations are given for each breeding line
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Example:
IR2061-214-3-8-2 (IR28)
Selections from the F2 were grown in the pedigree nursery as F3 rows:
IR2061-1, ------IR2061-214 -----and so on up to IR2061-806
From selection 214, three plants were grown as F4 selections:
IR2061-214-1, IR2061-214-2 and IR2061-214-3
From selection IR2061-214-3, 10 plants were again selected as F5 rows
These selections were designated as IR2061-214-3-1 up to 2061-214-310
From selection IR2061-214-3-8, three plant selections were made and
planted in the pedigree nursery as F6 rows IR2061-214-3-8-1, IR2061214-3-8-2 and IR2061-214-3-8-3
At maturity selection IR2061-214-3-8-2 appeared uniform and was bulk
harvested and evaluated in replicated trials
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How is the naming of pedigree
in your country/institute?
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Procedure
What are the breeding objectives?
•
•
•
•
High Yield Potential
Short Growth Duration
Superior Grain Quality
Multiple disease and insect resistance
Choice of Parents?
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Donors for various traits
(Hybridization Block)
Staggered sown 3 times each season
at 2-weeks interval
IRRI Varieties are donors in various programs as
 Irrigated
 Rainfed
 Flood prone and
 Hybrid rice
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Hybridization Block
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Hybridization Block
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Field Book
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Electronic Field Book
ENTRYNO DESIGNATION
RTV BPH1 GLH GELCON
SummaryBLofB1HBs
HB8401
HB8402
HB8403
HB8404
HB8405
HB8406
HB8407
HB8408
HB8409
IR 20
IR 29
IR 36
IR 43
IR 58
IR 64
IR 72
IR 74
PSB RC 10
7
6
6
6
5
5
7
3
2
1
1
1
1
1
1
1
6
1
87
60
81
85
58
71
75
79
85
8
4
2
8
2
3
2
2
2
8
4
6
7
4
5
4
4
4
44 L
94 L
42 HI/I
81 L
34 I/L
77 I
78 I/L
51 L
56 I/L
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GT
AMY GRL GRS CLK
25
2
25
18
26
23
26
26
25
5
3
3
3
5
3
3
3
3
5
1
1
3
5
1
1
1
1
2
0
3
5
1
4
2
5
4
Selected Donors
IR 65610-24-3-6-3-2-3
IR 67966-44-2-3-2
IR 68544-29-2-1-3-1-2
IR 68552-100-1-2-2
IR 76910-20-6 (xa 5,xa 13,Xa 21 )
IRBB 60-1
IR 69726-116-1-3
IR 73885-1-4-3-2-1-10
IR 73887-1-8-2-1
IR 73888-1-2-7
BG 90-2
IR 45912-9-1-2-2
IR 65620-192-3-3-3-2
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IR 68440-36-2-2-3
Planning Crosses!
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Further improvement in
Indica germplasm
 For Yield, grain quality and incorporation of
resistance to diseases and insects
Released varieties x Elite Indica Lines
Elite Indica Lines x Elite Indica Lines
Elite Indica Lines x Basmati Type Lines
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Developing
Basmati Type Germplasm
Basmati x Elite Indica Lines
Basmati x Basmati Type Derivatives
Basmati Type Derivatives x
Basmati Type Derivatives
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Improving Tropical Japonicas
for temperate regions (Korea)
Tropical Japonica x Tropical Japonica crosses
Tropical Japonica x Korean Varieties
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Further Improvement in NPTs
 For Yield, grain quality and incorporation
of resistance to diseases and insects
Improved NPTs x Improved NPTs
Improved NPTs x Released Indica Varieties
Improved NPTs x Elite Indica Varieties
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What crosses do you
currently focus on in
your institute?
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F1 Nursery
Types of crosses:
Single crosses
Backcrosses
Three way/Top crosses
Around 900 crosses yearly (wet & dry season)
Cross designation (IR80369)
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F1
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Field Operations
Grow 2-6 rows (30 Plants) /cross
Rouge off-types
Identify accidental selfs
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F2 Nursery
Protected:
 In general susceptible to diseases and insects
Unprotected:
 In general resistant to diseases and insects
First grow in blast nursery &
Reject Susceptible populations
Space planted 35 x 25 cm.
Population size from 2,500- 5,000 individuals
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Blast Nursery
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Blast Nursery
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Blast Nursery
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Protected F2
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Unprotected F2
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Field Operations
• Inoculate with BB Race1/Race2
• Rogue susceptible/undesirable plants
• Harvest desirable plants (100-500 plants)
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Pedigree nursery
Four nurseries/year:
• January & July (Unprotected)
• May & November (Protected)
 Space planted 30 x 20 cm
 Every 20th row is a check variety (4 Checks)
 16,000 to 20,000 rows (30 plants)
What system do you
have in your institute?
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Pedigree Nursery in May
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Pedigree Nursery in May
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Pedigree Nursery in July
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Pedigree Nursery in July
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DATE : 2002-04-23
Cross
Parents
PN MAY 2002 SCREENING REQUIREMENTS
A G
A
G
Start End Sel. Gen. B P H L B B B R GEL M R
1 2 3 H L 1 2 O TEMP Y E
F9
80001 80003 3
X X X
X
IR 74713 IR 69807-13-2-1
Screening requirements
Memo IR 69715-36-1-1
IR 75261 IR 65600-38-1-2-1 80004 80006
PSB RC 64
IR 75263 IR 66158-38-3-2-1 80007 80012
IR 68058-64-1-2
IR 75266 IR 66158-38-3-2-1 80013 80015
IR 68450-36-3-2-2-3
3
F8
6
F8
3
F8
X
X X X
X
X
X
X X X
X
X
X
X X
X
X
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X
Field Book
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F3 single rows
F4 onwards 3/6 rows
Selection between rows/within rows- at
least 3 plants.
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17811 Progeny Rows
Composition of 2004 NOV PN
Gen
Cross type
No.
Gen
Cross type
No.
F3
I/I/I
4
F5
I/I
5
IND/IND
18
I/I/I
2
IND/WH
3
I/J/I
18
JAP/BAS/ARO
4
J/I
6
JAP/I/I
1
J/J
3
Jap/Korean
10
Micronutrient
21
NPT/IND
19
I/I
17
NPT/JAP/IND
3
NPT/I
32
I/I
11
NPT/NPT
17
I/I/I
1
J/I/I
18
I/J
31
Micronutrient
40
NPT/BAS
6
I/J
2
NPT/I/I
5
Micronutrient
20
NPT/NPT
14
NPT/NPT/I
3
F4
F6
F7
IRRI: Planning Breeding Programs for Impact
2004 NOV PN
Cross
Parents
Start
End
Sel
.
Gen
.
Type
IR 79228
IR 72903-6-2-2-1
46001
46006
6
F7
I/J
46007
46009
3
F7
I/J
46010
46015
6
F6
I-NPT/NPT
46016
46018
3
F6
I-NPT/NPT
46019
46037
18
F6
I-NPT/NPT
46038
46050
12
F6
I-NPT/NPT
46051
46053
3
F6
I-NPT/NPT
46054
46072
18
F6
NPT/NPT
IR 67961-15-1-1
46073
46082
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9
F6
NPT/NPT
IR 67966-44-2-3-2
IR 79232
IR 72905-49-3-2-2
IR 68552-100-1-2-2
IR 79483
IR 71700-247-1-1-2
IR 68544-29-2-1-3-1-2
IR 79484
IR 71700-247-1-1-2
IR 72225-20-3-2-3
IR 79485
IR 71700-247-1-1-2
IR 73707-45-3-2-3
IR 79487
IR 72158-11-5-2-3
IR 72225-20-3-2-3
IR 79489
IR 72967-12-2-3
IR 68544-29-2-1-3-1-2
IR 79493
IR 67961-15-1-1
IR 68544-29-2-1-3-1-2
IR 79494
IR 72225-29-1-2-1
OYT
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RYT
IRRI: Planning Breeding Programs for Impact
Any questions or comments?
IRRI: Planning Breeding Programs for Impact