Zebrafish, Danio rerio, Husbandry, Egg to Egg, the Do’s

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Transcript Zebrafish, Danio rerio, Husbandry, Egg to Egg, the Do’s

The ZIRC Nursery

The Basics of a Successful Zebrafish Nursery System

European Zebrafish Meeting 2009 Rome, Italy Carrie L. Barton Zebrafish International Resource Center University of Oregon

ZIRC Autonursery System

• Modular rack system • Fully automatic water delivery • 500 ml polycarbonate tanks independent, adjustable water inlet Capacity Functional Capacity = 12,000 fish Total Capacity = 24,000 fish Density = 50 fish per tank

ZIRC Nursery Feeding and Water Schedule

• Water Flow Progression: Static-12hr-24hr • Food Progression and Size Overlap: Paramecia-Powder-Artemia

The Fundamentals

Goal: Embryo to Adult Effectively and Efficiently • Water Quality • Food Quantity • Food Size

Water Quality

Pre-nursery

• Embryo Medium: Modified E2 • Egg Quality •

Schedule

• Static vs. Running Water • This will largely depend on your particular system •

Flow Rate

• Clean Water

vs.

• Food Retention/Wash Out

Food Quantity

Relationship to Water Quality

• • Running Water = More Food Static System = Requires More Precise Food Delivery •

Number of Feedings Per Day

• 2-3 is Ideal •

Amount Administered at Each Feeding

• Density of Fish/Size of Tank • • • Avoid Overfeeding •

Overfeeding = Excessive Food Build Up = Poor Water Quality

Powdered Feed and Artemia Can Adversely Effect Your Water Quality if Over Fed Paramecia and Rotifers Can be Fed More Liberally

Food Choice

Commonly Use Foods:

• •

Paramecia

• •

Rotifers Artemia Nauplii Powdered Larval Diets

Live vs. Powdered Feeds

Brine Shrimp Pros: -Readily available -Easy to culture -Transition food Paramecia/Rotifers Pros: -Can be fed @ 4 dpf -Clean food -No overfeeding issues -Stays in water column Cons: -

Can adversely effect water quality if overfed -Large size limits when it can be fed -Quality is variable season to season

Cons: -

More labor intensive than powdered food -Small size limits when it can be fed

Powdered Food Pros: -Wide size range -Commercially Available -Not labor intensive -Transition food Cons: -

Can adversely effect water quality if overfed -Limited time in water column

Food Size

Food Particle Size vs. Fish Mouth Size

Food Size

Appropriate Size Range and Overlap

Paramecium Caudatum < Brine Shrimp (SFB) < Powdered Feeds 250 x 50 µm < 400 x 200 < 100-450 (Diameter)

Take Home Message

All Roads Lead to Rome.

” Translation: Different Paths Can Take One to the Same Goal.

The Three Goals

Water Quality

Food Quantity

Food Size -Be Ready and Willing to Observe and Make Modifications-

Contact Information

Zebrafish International Resource Center University of Oregon Web Address: www.zebrafish.org

Email: [email protected]

Phone: (541) 346-6028

Environmental Parameters

• • • • • • • •

Lighting: 14 hours/day Temperature: 28.5˚C (22-30˚C tolerated) pH: 7.2 to 7.6

Conductivity: 500 1500 µS Ammonia: 0 ppm Nitrite: 0 ppm Nitrate: < 10 ppm Exchange rate: 10% per day (RO water)