Transcript A Zebrafish Holding Chamber for Use with a Micro CT Scanner

A Zebrafish Holding Chamber for
Use with a Micro CT Scanner
University of Wisconsin - Madison
Biomedical Engineering Design Courses
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Team Members
Austin Ramme, Kristen Sipsma,
Andrew Neumann, and Mike Haggerty
Client
Jill M. Kolesar, Pharm.D., BCPS
Associate Professor of Pharmacy
Advisor
Professor Justin Williams
Presentation Outline
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Problem Statement
Background Information
Current Devices
Design Specifications
Alternative Designs
Final Design
Future Work
Problem Statement
 Client
– An Evaluation of Carcinogenesis in a Zebrafish Model
– Ultimate Goal: Cure Cancer
 Design Team
– Design a Zebrafish Holding Chamber
– Compatible with a Micro CT Scanner
– Sustains Zebrafish Life
Background Information
 CT = Computed Tomography
 Benefits of Zebrafish Research
– Quick Breeders
– Low Maintenance
– Mapped Genome
 Zebrafish Anatomy
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Length: 1-6cm
Lifespan: 2 years
Water Temp: 64-75° F
Bony Fish: Breathe Without Moving
Typical CT Scanner
Current Devices
 Market Products
– Rodents
– Zebrafish
Rodent Tray
 Client’s Device
– Cardboard
– Zebrafish Slits
– High Mortality Rate
Client’s Current Device
Preliminary Experimentation
 February 11, 2004 Conclusions
– Survival: 1.5 mL for >30 min
– Increased Breathing Rates
– Preferred Orientation
– No Adverse Behavior in Cuvette
– No Adverse Behavior with Restraint
Design Specifications
 No leakage
 Zebrafish must remain
alive for 20 minutes
 Metal, glass, and dense
materials must not be
used
 Minimize all motion of the
zebrafish
 Disposable or
autoclavable
Micro CT Scanner
Design 1: Falcon Tube
 Falcon tube filled with
water with sponge
inside
 Small slits to hold fish
 One to two fish per
tube
 Sponge absorption
Design 2: Plexiglas Container
Side View of Plate in Container
Thumb – Screws
3D View of Container – No Plates In Place
Multiple Fish
Adequate Water
Contamination
Water
65mm
65mm
Design 3:
The Cuvette
Cuvette
Stabilization of Fish
Alignment Tray
Disposable/Inexpensive
Water Supply
Space Efficient
Design Matrix
Criteria:
Cuvette
Design
Plexiglas
Container
Falcon Tube with
Sponge
Cost
4
3
3
Ease of Use
3
3
2
Constructability
5
3
4
Effectiveness:
Immobilization
4
4
3
Effectiveness:
Survival Rate
5
5
4
Totals
21
18
16
Each criterion was rated on a 0 to 5 scale with 5
being the best.
Future Work
 Cuvette Tops
– Rubber Stopper
– Market
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Sizing Difficulties
Opaque Materials
Build Prototype
Prototype Testing
Questions ?