A Zebrafish Holding Chamber for Use with a Micro CT Scanner

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Transcript A Zebrafish Holding Chamber for Use with a Micro CT Scanner

A Zebrafish Holding Chamber for
Use with a Micro CT Scanner
University of Wisconsin - Madison
Biomedical Engineering Design Courses
INTELLECTUAL PROPERTY STATEMENT
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subsequent presentations is the property of the University and of the researchers
presenting this information. In addition, any information provided herein may
include results sponsored by and provided to a member company of the
Biomedical Engineering Student Design Consortium (SDC). The above information
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1) Agrees to use this information solely for purposes related to this review;
2) Agrees not to use this information for any other purpose unless given written
approval in advance by the Project Group, the Client / SDC, and the Advisor.
3) Agrees to keep this information in confidence until the University and the
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4) Continued attendance at this presentation constitutes compliance with this
agreement.
Team Members
Austin Ramme, Kristen Sipsma,
Andrew Neumann, and Mike Haggerty
Client
Jill M. Kolesar, Pharm.D., BCPS
Associate Professor of Pharmacy
Advisor
Professor Justin Williams
Presentation Outline
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Problem Statement
Background Information
Current Devices
Design Specifications
Alternative Designs
Final Design
Future Work
Problem Statement
 Client
– An Evaluation of Carcinogenesis in a Zebrafish Model
– Ultimate Goal: Cure Cancer
 Design Team
– Design a Zebrafish Holding Chamber
– Compatible with a Micro CT Scanner
– Sustains Zebrafish Life
Background Information
 CT = Computed Tomography
 Benefits of Zebrafish Research
– Quick Breeders
– Low Maintenance
– Mapped Genome
 Zebrafish Anatomy
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Length: 1-6cm
Lifespan: 2 years
Water Temp: 64-75° F
Bony Fish: Breathe Without Moving
Typical CT Scanner
Current Devices
 Market Products
– Rodents
– Zebrafish
Rodent Tray
 Client’s Device
– Cardboard
– Zebrafish Slits
– High Mortality Rate
Client’s Current Device
Preliminary Experimentation
 February 11, 2004 Conclusions
– Survival: 1.5 mL for >30 min
– Increased Breathing Rates
– Preferred Orientation
– No Adverse Behavior in Cuvette
– No Adverse Behavior with Restraint
Design Specifications
 No leakage
 Zebrafish must remain
alive for 20 minutes
 Metal, glass, and dense
materials must not be
used
 Minimize all motion of the
zebrafish
 Disposable or
autoclavable
Micro CT Scanner
Design 1: Falcon Tube
 Falcon tube filled with
water with sponge
inside
 Small slits to hold fish
 One to two fish per
tube
 Sponge absorption
Design 2: Plexiglas Container
Side View of Plate in Container
Thumb – Screws
3D View of Container – No Plates In Place
Multiple Fish
Adequate Water
Contamination
Water
65mm
65mm
Design 3:
The Cuvette
Cuvette
Stabilization of Fish
Alignment Tray
Disposable/Inexpensive
Water Supply
Space Efficient
Design Matrix
Criteria:
Cuvette
Design
Plexiglas
Container
Falcon Tube with
Sponge
Cost
4
3
3
Ease of Use
3
3
2
Constructability
5
3
4
Effectiveness:
Immobilization
4
4
3
Effectiveness:
Survival Rate
5
5
4
Totals
21
18
16
Each criterion was rated on a 0 to 5 scale with 5
being the best.
Future Work
 Cuvette Tops
– Rubber Stopper
– Market
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Sizing Difficulties
Opaque Materials
Build Prototype
Prototype Testing
Questions ?