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WVU Shared Research Facilities

Siera Talbott, PhD Lisa Sharpe September 17, 2012

Shared Research Facilities Family

 Cleanroom –  Kolin Brown Engineering Sciences Bldg (Evansdale)  Electron Microscopy –  Marcela Redigolo Engineering Sciences Bldg (Evansdale)  Materials Fabrication and Characterization Facility (MFCF) – Wei Ding  Engineering Sciences Bldg (Evansdale)  White Hall (Downtown)  Bio-Nano Research Facility –   Siera Talbott G75E Engineering Sciences Bldg (Evansdale) 381 Chemistry Research Laboratory Bldg (Downtown) SharedResearchFacilities.WVU.edu

Cleanroom

Specialized laboratory environment for fabricating and working with micro and nano scale devices or structures SharedResearchFacilities.WVU.edu

Dr. Kolin Brown (304-293-9683) [email protected]

Cleanroom

      Lithography  Photolithography  E-beam lithography Wet processing Deposition  E-beam evaporation   Sputtering PECVD Dry etching  Reactive Ion Etching  Oxygen Plasma Asher Thermal processing  Rapid thermal annealing  Furnaces Packaging Dr. Kolin Brown (304-293-9683) [email protected]

SharedResearchFacilities.WVU.edu

Cleanroom - Applications

 Electrode array test beds  Microfluidic chips and channels  Functionalized surfaces for biological sample attachments  Optical waveguide sensors  Electronic devices  LEDs Dr. Kolin Brown (304-293-9683) [email protected]

SharedResearchFacilities.WVU.edu

Electron Microscopy - TEM



2 nm

     Transmission Electron Microscope JEOL JEM 2100 Structural Characterization Thermo source (LaB6 filament) Operates at 120 KV (

for more sensitive samples / bio samples

) and 200 KV ( for other materials )

Resolution: 1.4 Å (lattice / atomic planes)

EDX capabilities (Chemical elements)  Two digital cameras:  Gatan ES500W (high speed + high quality imaging w/ field of view larger than traditional / allow imaging of intense electron diffraction patterns w/o blooming artifact)  Gatan Orius SC 1000 – ultra high quality in high resolution Dr. Marcela Redigolo (304-293-9973) [email protected]

SharedResearchFacilities.WVU.edu

Electron Microscopy - SEM

Dr. Marcela Redigolo (304-293-9973) [email protected]

       Scanning Electron Microscope JEOL JSM 7600FE In-lens thermal FEG (generates probe current 10x higher than conventional) Aperture angle control lens automatically optimizes the spot size Image topography/surface of majority of materials.

Gentle Beam mode: for sensitive

samples (biological or non conductive)

Resolution: 1 nm (15KV) EDX capabilities including X-ray mapping of the specimen SharedResearchFacilities.WVU.edu

Electron Microscopy - Sample Prep

  MultiPrep Precision Polishing System  Parallel Polishing   Precise Angle Polishing Site-specific Polishing Support instruments:      

Prepare samples for TEM, SEM, AFM, and more

Quantify material removal Optical Microscope  CO2 Critical Point Dryer Hot Plate / Stirrer  Low Speed Saw (up to 500 rpm)  Dicing / Cutting Saw Prepare biological samples for SEM.

Cut different types of wafers with precision Dr. Marcela Redigolo (304-293-9973) [email protected]

SharedResearchFacilities.WVU.edu

Materials Fabrication & Characterization Facility (MFCF)

• • • • • • • • • Atomic Force Microscopy (AFM) Fourier Transform Infrared Spectrometer (FTIR) Pulsed Laser Deposition System (PLD) Raman Spectroscopy (Raman) RF/DC Magnetron Sputtering System Spectroscopic Ellipsometer X-ray Diffractometer (XRD) X-ray Photoelectron Spectrometer (XPS) Ultraviolet Photoelectron Spectroscopy (UPS) Dr. Wei Ding (304-685-1938) [email protected]

SharedResearchFacilities.WVU.edu

Materials Fabrication & Characterization Facility (MFCF)

• Raman Micro-spectroscopy • Determining chemical composition, molecular structure and molecular interactions • Quantitative analysis of material chemical compositions • AFM (Atomic Force Microscopy) • Adhesion and friction forces measurements between surfaces in contact • Conductivity and magnetic property measurements at nanoscale Dr. Wei Ding (304-685-1938) [email protected]

SharedResearchFacilities.WVU.edu

•

Materials Fabrication & Characterization Facility (MFCF)

XPS (X-ray Photoelectron Spectroscopy) • Micro-area element composition determination • • Chemical state determination at surface Depth profile analysis of structures and interfaces • XRD (X-ray Diffractometry) • Determining crystal structure of solids, powders and thin films • Reciprocal space mapping of crystalline materials and 2D mapping of wafers Dr. Wei Ding (304-685-1938) [email protected]

SharedResearchFacilities.WVU.edu

Bio-Nano Research Facility - Equipment

• • • • • Mammalian cell culture Sample preparation & analysis Microscopy • Optical Profiler • • Fluorescent, brightfield and DIC Video Spectroscopy • Mass spectrometry • Fluorescence spectrometry Sterilization Dr. Siera Talbott (304-293-0747) [email protected]

SharedResearchFacilities.WVU.edu

Bio-Nano Research Facility

• Mammalian cell culture • Cellular viability and toxicity assays • Microscopy Dr. Siera Talbott (304-293-0747) [email protected]

SharedResearchFacilities.WVU.edu

Bio-Nano Research Facility – Mass spec

SharedResearchFacilities.WVU.edu

C:\XCALIBUR\...\20120328carbamazepine01 3/28/2012 3:51:06 PM RT: 0.00 - 10.00

100 90 80 70 60 50 40 30 20 10 0.06

0.38

0 0.0

0.5

1.98

2.05

0.79

1.27

1.56

1.81

1.89

1.0

1.5

2.0

2.56

2.73

2.90

3.12

3.44

3.70

4.01

4.38

4.68

4.81

4.86

4.93

5.03

5.38

5.56 5.68 5.93 6.19

2.5

3.0

3.5

4.0

4.5

5.0

Time (min) 5.5

20120328carbamazepine01 # 1415 RT: 3.32

AV: 1 NL: 2.11E6

T: FTMS + p ESI Full ms2 [email protected] [50.00-505.00] 100 90 80 70 60 50 40 30 20 10 0 69.07

81.07

95.09

109.10 121.10

147.12

189.13

161.13 172.98

191.14

195.09

213.01

201.16

60 80 100 120 140 160 180 200 220 236.16

238.16

240 249.04

261.08

277.09

260 280 m/z 293.07

300 6.0

321.12

320 6.5

6.45

6.71 6.99 7.42

340 7.0

360 7.5

380 7.87

8.0

400 8.28

8.48

8.89 9.03

8.5

420 436.78

440 9.0

460 9.52

9.71

9.5

480 10.0

NL: 5.23E7

TIC MS 20120328c arbamazep ine01 504.46

500

How to become a SRF user?

www.sharedresearchfacilities.wvu.edu

 FOM software  User agreement for each facility  Safety trainings  Equipment trainings SharedResearchFacilities.WVU.edu

Usage

Fees

 Most equipment is billable by hour – http://sharedresearchfacilities.wvu.edu/srfCosts.html

Per Hour WVU Users

$21

WV Academic Users

$25

Non-Profit Organizatio n Users

$31

External Users

$33

SEM Assisted SEM Unassisted TEM Assisted TEM Unassisted

$6 $65 $50 $10 $125 $96 $13 $156 $120 $18 $230 NA SharedResearchFacilities.WVU.edu

2012 Support

User Fees by Facility

    Cleanroom $73,740 Electron Microscopy $52,700 Materials Fabrication and Characterization $28,766 Bio-Nano Research $755

Other Support

      ECAS $72,406 BSCEMR $72,406 Provost $110,000 HEPC $100,000 WVU/WVURC $139,000 RII Grant Support for BNRF $931,241

Anticipated 2013 Support

User Fees by Facility

    Cleanroom $71,250 Electron Microscopy $58,000 Materials Fabrication and Characterization $30,000 Bio-Nano Research $25,000

Other Support

     ECAS $34,398 BSCEMR $51,597 HEPC $100,000 WVU/WVURC $294,013 RII Grant Support for BNRF $365,351 For additional information, please contact: Lisa Sharpe, Assistant Director of Program Operations, WVU SRF [email protected]

304-293-6872

THANK YOU!!