Transcript Custom Recombinant Protein Production

Custom Recombinant Protein
Production
Fitzgerald Industries International
30 Sudbury Rd, Acton,
MA 01720, USA
Phone: +1-978-371-6446
Email: [email protected]
Our Custom Protein Manufacturing Service
 ISO 9001:2008 Certified State-of-the-Art facility
 Small to Large Scale Manufacturing Capabilities
 Proprietary 3 Step Sequential Purification
 Technique guarantees >95% purity
 Expression Systems include E.coli and Insect Cells
 Sample Provision for Assay Validation
Our Expert Hands-on Approach
 Direct and constant contact with our expert technical
scientists
 Customization of our processes, reagents, and timelines
to suit your needs
 Custom quotes depending on what you require
 Multi-stage end products – from a purified plasmid to
full scale bulk protein production.
Our Protein Production Processes
 Step by Step Guide to our recombinant protein
manufacturing in E.coli and Insect Cell expression systems
 Download our schematic representations:
Step by Step Guide to Recombinant Protein Production in E.coli
Step by Step Guide to Recombinant Protein Production in Insect Cells
 Or watch on for an outline of both processes….
Protein Production using E.Coli Expression
System
Phase
Description
Timeline
1
Subcloning into Expression Vector
3-4 weeks
2
Generation and Identification of
Expression Positive Clones
2 weeks
3
Expression Optimization
2-3 weeks
4
Large-scale Culture
1 week
5
Protein Extraction & Purification
2-4 weeks
View the full process on our Custom Protein
Production section on our website
Protein Production using Insect Cells
Expression System
Phase Description
Timeline
1
Subcloning into Recombinant Baculovirus
Transfer Vector
3-6 weeks
2
Generation & Evaluation of Baculovirus
Recombinants
2-4 weeks
3
Amplification of Baculovirus Stock
2-3 weeks
4
Baculovirus Expression Optimization Service
1 week
5
Protein Production Service
2-3 weeks (1-5L),
4-5 weeks (5-20L),
8-10 weeks (20L)
6
Protein Extraction & Purification
2-3 weeks
View the full process on our Custom Protein
Production section on our website
Downloads & Useful Links
 Our website: www.fitzgerald-fii.com
 Direct link to Custom Protein Production page
 Infographics:
Recombinant Protein Production in E.coli
Recombinant Protein Production in Insect Cells
 Download our Custom Protein Manufacturing Flyer
Contact Us:
[email protected]
USA: + 1-978-371-6446
Europe: + 353-1-272-4455
Fitzgerald Industries International
30 Sudbury Rd, Acton, MA 01720, USA.