Transcript Clinical pathology: SEROMARKER FOR TB

Clinical pathology:
SEROMARKER FOR TB
GENERAL OBJECTIVE:
After finishing lab activity of Seromarker TB, the student
will be able to describe the Seromarker TB in the DMS case
problem
SPECIFIC OBJECTIVE:
At the end of lab activity of Seromarker TB, the student
will be able to interprete:
- the normal of Seromarker TB
- the abnormal of Seromarker TB
Introduction
• Tuberculosis : Chronic infectious disease
M. tuberculosis
 involves multiple organs
 >> lung
• Indonesia: number 3 in the world
after India and China
• West Java Health Profile 2000:
 The caused of morbidity (no 4) in the hospital
 Number 2 the caused of mortality
Transmission
• droplet nuclei
size 1-5 µm
1-3 AFB
• Source
of infection
Patients with
Sputum AFB (+)

Diagnosis:
- Direct smear:Ziehl Nielson: Acid fast
bacilli
- Culture: GOLD STANDARD
- Tuberculin test
- Chest X ray (Pulmonary TB)
Mycobacterium tuberculosis
Problem?
 Culture: 4-6 weeks
 Not available in all center/laboratory
 Extra pulmonary TB
 Smear negative Pulmonary TB
 Children
SEROMARKER TB/Immunological test
Detection of antibodies againts TB
Seromarker TB


Anti Myc. TB ELISA
ICT TB
- A chromatographic immunoassay for
qualitative detection of antibodies againts TB
in human serum or plasma
- The test employing recombinant antigen
38kD, 16 kD and 6 kD (ESAT-6),
lipoarabinomannan
- The use of a collection of antigen
improves the sensitivity of serological test
Seromarker TB

Mycotec TB( recombinant) employs
the complexes conjungate with gold
colloidal particle passes over the
immobilized multiple of recombinant
TB ag precoated on the test area (T)
once the patient sample is added to
the sample well.
Principle of the test
 If
there are some ab to TB
are present in patient sample
then the TB recombinant ag
capture them, a pink/purple
band is produced on the test
area.
 The
migration of remaining
complexes which were not
bound by those TB ab, will be
then immobilized to the control
area (C).
 A pink/purple band is appeared
on control area, the band on
control performs a properly test
Prosedur Pengujian
1. Pipetkan 100 μl serum atau plasma
2. Baca hasil 5-20 menit setelah penetesan
spesimen
Interpretasi Hasil
Positif
Tampak 2 garis warna merah muda atau ungu di area
Test (T) dan Kontrol (C)
Negatif
Tampak 1 garis warna merah muda atau ungu di area
Kontrol (C)
Invalid
Tidak tampak garis di area Kontrol (C)
Mekanisme Reaksi