Transcript Title

It’s a Mystery!
The Case of the Scientist Slayer
Katelin Eastman, Kelsey Moore, Hannah
Stork, and Natalie Hansen
Period 2
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It was a dark and windy night…
All the professors at Monarch
University appeared to have gone
home. All, that is, except those in
(and acquainted with) the Monarch
science department. Their raging
party, hosted by the infamous Dr.
Doodles Donley, could be heard
echoing throughout the halls of the
prestigious university…
Suddenly...
Guests began to leave (slowly at first), but
after a while, those who could not hold the
orange marmalade were forced to quickly
vacate the area.
The only guests who remained were Dr.
Lazarus Dangerkitty, Dr. Sloopy Stretch, Dr.
Sandro Music, and Dr. Red Walrus
Meanwhile…
The great geniuses at the Secret Science Squad
(S.S.S.) received a frantic phone call
CALLER:
Please…send
someone…anyone…help…Donley…d
dddead…quickly.
The wind that night was so strong that the call
was interrupted and sporadic.
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AGENT MOORE: Oh no! We have to help them. Dr.
Donley is dead!
AGENT STORK: Hansen, prepare our Secret Science
Speedracer – we’ll have to get there as fast as we
can.
AGENT HANSEN: Right away, Stork. Perhaps we should
take the Secret Science Spaceship instead – it can go
up to 500 mph. We’ll be able to get there in .025
seconds.
AGENT STORK: Of course! How could I forget the
Spaceship?
The agents flew at great speed, eventually
arriving at the crime scene in no time at all.
AGENT MOORE: By golly! We almost forgot
Eastman. Where is that wily scientist?
AGENT EASTMAN: Right here, Moore. I was
just preparing some sacs for our evidence.
Then we can run some tests. Here’s an
outline for our eventual report – now all we
have to do is fill in the blanks.
AGENTS: Let’s crack this case…
AGENT HANSEN: …The Case of the Scientist
Slayer.
On the Trail of a Killer
 The geniuses of the S.S.S. entered
the lab and spent careful time
collecting every piece of evidence,
even the most trivial of items.
 The following is an accurate account
of what they discovered.
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Evidence
 The Secret Science Squad encountered
lots of blood at the scene of the
crime, both on Dr. Donley and the
insurance policy.
 After swabbing and analyzing the
samples, they found two blood types;
O+ and B+.
 People with O+: Walrus, Sheetburger,
Moose.
 People with B+: Powers, Dangerkitty
 Donley has O+, there is a possibility
of this sample being her blood.
Prints
 Three glasses had lip prints on them
at the party
 They matched the lips of Donley,
Music, and Dangerkitty
 Fingerprints were found on the
incubator in Donley’s lab as well as
on test tubes
 The fingerprints belonged to One Eyed
Pete and Dr. Donley
 Fingerprints were also found on
goggles in the lab, belonging to Dr.
Kelvin High
www.idfpr.com
Autopsy Report
 Dr. Donley was poisoned by
Cyanide found in her cocktail.
 Time of death is estimated to be
between 10 and 11 p.m.
 Blood not belonging to Dr.
Donley was found on her right
hand, beneath her fingernails,
and on her clothes.
Other Evidence
 Dog hair was also found at the crime
scene. Dr. Dangerkitty, Dr. Music,
and Dr. Walrus all have dogs.
 There was also a crumpled letter at
the crime scene…perhaps a love
letter…ooohhhh….
 There was also a ripped out diary
page
 The room smelled faintly of almonds
What next?
 How, you ask, do the geniuses of the S.S.S.
interpret this evidence?
It’s really quite simple
(if you’re a genius)
 There are several techniques that the
agents use, including, but not limited to:
DNA Fingerprinting, ELISA Tests, fingerprint
analysis, and blood tests.
Blood Typing
 A person’s blood type is classified based on the
presence or absence of inherited antigenic substances
(proteins,carbohydrates, glycoproteins, or glycolipids)
 Blood type is inherited from one’s parents and is
controlled by multiple alleles
 The ABO blood group system and the Rhesus blood
group system are the most common
 Antigen A = type A
 Antigen B = type B blood
 No antigens = type O blood
 Antigens A and B = type AB
 RhD present = positive
 RhD absent = negative
http://en.wikipedia.org/wiki/Blood_type
Blood Type Chart
Blood type is partially determined by the ABO blood group antigens
present on red blood cells.
http://en.wikipedia.org/wiki/Blood_type
DNA Fingerprinting
 DNA fingerprinting is a process to show
relatedness or identity of individual
humans, plants, or animals.
 Every person is not only genetically
different but also different in their introns.
 It analyzes STR (single tandem) repeats
which are four to six base pairs long and
repeat on specific chromosomal loci. By
determining the odds of a certain STR
repeat, suspects can be eliminated.
DNA fingerprinting scenarios
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Food identification
Accused and convicted felons
Identifying human remains
Paternity tests
Identifying pathogens
www.accelrys.com
DNA Fingerprint Procedure
1.
2.
3.
4.
5.
6.
7.
8.
9.
Obtain samples from the crime scene and suspects and
place in test tubes
Add 10 microliters of enzyme mix ENZ into the bottom of
each tube
Mix the components
Incubate the tubes at 37 degrees Celsius for 45 minutes.
Prepare agarose gel for electrophoresis
Load 15 microliters of each sample into the wells of the gel
and record their order.
Place the lid on the elcetrophoresis chamber, plug in the
jacks to the base, and turn on the power for 30 minutes at
100 volts.
Finish the process of electrophoresis (described elsewhere)
Analyze results
DNA Fingerprint Lab analysis
 The DNA at the crime scene
matched that of Suspect Three,
Dr. Red Walrus, because the STR
repeats shown by electrophoresis
were identical.
Hmmm…I think the S.S.S. is on to
something
ELISA Tests
 Enzyme-linked immunosorbent assay
(ELISA) tests rely on antibodies to
detect the presence of antigens in
liquid samples.
 They provide agents with the ability
to detect minute amounts of disease
agents in samples (usually body
fluids)
Uses for ELISA tests
 Detection of…
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Smallpox
West Nile
HIV coat protein p24
SARS
Anthrax spores
Hormones
Steroids
Bacteria
ELISA Procedure
1.
2.
3.
4.
5.
6.
7.
Add sample and control samples to wells in a
microplate strip
Incubate for 5 minutes
Add primary antibody to the wells and incubate
Detect the bound antibodies with an HRP-labeled
secondary antibody
Add enzyme substrate to the wells
Wait 5 minutes
Analyze wells
 If the well turns blue, there antigen is present
 If the well is colorless, there is no antigen
ELISA Lab analysis
After conducting the ELISA test on the
evidence, the agents of the S.S.S.
came to the following conclusion:
DR. RED WALRUS HAS SMALLPOX
www.texasrealtoronline.com
Smallpox – The Disease

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Smallpox is a serious, contagious, and sometimes fatal infectious disease.
There is no specific treatment for smallpox disease, and the only prevention
is vaccination. The name smallpox is derived from the Latin word for
"spotted" and refers to the raised bumps that appear on the face and body
of an infected person.
There are two clinical forms of smallpox. Variola major is the severe and
most common form of smallpox, with a more extensive rash and higher
fever. There are four types of variola major smallpox: ordinary (the most
frequent type, accounting for 90% or more of cases); modified (mild and
occurring in previously vaccinated persons); flat; and hemorrhagic (both
rare and very severe). Historically, variola major has an overall fatality rate
of about 30%; however, flat and hemorrhagic smallpox usually are fatal.
Variola minor is a less common presentation of smallpox, and a much less
severe disease, with death rates historically of 1% or less.
Smallpox outbreaks have occurred from time to time for thousands of
years, but the disease is now eradicated after a successful worldwide
vaccination program. The last case of smallpox in the United States was in
1949. The last naturally occurring case in the world was in Somalia in 1977.
After the disease was eliminated from the world, routine vaccination against
smallpox among the general public was stopped because it was no longer
necessary for prevention.
Smallpox (cont’d)
Where Smallpox Comes From
 Smallpox is caused by the variola virus that
emerged in human populations thousands
of years ago. Except for laboratory
stockpiles, the variola virus has been
eliminated. However, in the aftermath of
the events of September and October,
2001, there is heightened concern that the
variola virus might be used as an agent of
bioterrorism. For this reason, the U.S.
government is taking precautions for
dealing with a smallpox outbreak.
Smallpox - Transmission
Transmission
 Generally, direct and fairly prolonged face-to-face contact is
required to spread smallpox from one person to another.
Smallpox also can be spread through direct contact with
infected bodily fluids or contaminated objects such as
bedding or clothing. Rarely, smallpox has been spread by
virus carried in the air in enclosed settings such as
buildings, buses, and trains. Humans are the only natural
hosts of variola. Smallpox is not known to be transmitted by
insects or animals.
 A person with smallpox is sometimes contagious with onset
of fever (prodrome phase), but the person becomes most
contagious with the onset of rash. At this stage the infected
person is usually very sick and not able to move around in
the community. The infected person is contagious until the
last smallpox scab falls off.
The plot thickens…
 After much time and deliberation, the
S.S.S. felt that they had gathered
sufficient evidence to begin
questioning the suspects.
 Many resisted their inquiries, but
were dismissed because of strong
alibis. There were only four who
caught the attention of the agents.
Prime Suspects
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Dr.
Dr.
Dr.
Dr.
Lazarus Dangerkitty
Red Walrus
Sandro Music
Sloopy Stretch
DR. LAZARUS DANGERKITTY
 ALIBI: “I was in my lab until
11:30…all alone. Just me and my
plastic robo-cats! Only the security
guard saw me leave.”
 MOTIVE: “I didn’t like her, not one
bit. (heh, heh, heh) She stole all
of my ingenious lab ideas!”
 OTHER NOTES: It appears that Dr.
Dangerkitty suffers from multiple
personality disorder and has hints of
bipolar mood swings.
DR. RED WALRUS
 ALIBI: “I was at school in my lab. Alone,
all alone I tell you!”
 MOTIVE: (evil laugh) “I love it that she’s
dead! Now my world is complete. Dr.
Donley was always after my stinky brew, but
now no one will be able to get it!!!!!!
The Nobel Prize is now in my reach. I can
see it now - me, the prize, and my dogs.
You ask why I need my brew? What is it
exactly? That you will never know, only
that it is for all the voices in my head!
(Muah ha ha!)
 OTHER NOTES: Walrus seemed very nervous and
exhibited the signs of the early stages of
schizophrenia.
DR. SANDRO MUSIC
 ALIBI: “11:00, hmmm…oh yes, I was
giving my 3 year old a bath, she had a
run-in with chocolate syrup.”
 MOTIVE: “I’m glad that Donley is dead,
however, I am saddened that the
scientific community has lost one of
its most brilliant minds. But yes,
I’m glad she’s gone because Donley had
no appreciation for the finer things
in life, like music (tra la la).”
 OTHER NOTES: Claimed she would never
kill and has two very furry dogs
Dr. Sloopy Stretch
 ALIBI:
(NOTE: AGENT STORK has a hidden talent for
detecting lies and informed us that Stretch was
speaking in a juvenile language called “Opposite”)
“I was at home, I wasn’t outside her
window looking in. Yep, it was just
me and my cat DonLee, we weren’t
peeping at her…”
 OTHER NOTES: Stretch was in Donley’s
lab after hours. It seems that he
was satisfying his huffing habits and
was contributing to his obvious sick
adoration of Dr. Donley. It appears
as if he was “fondling her pencils.”
So…What really happened?
 After many sleepless nights, the geniuses
of the S.S.S. came to their final conclusion.
We now join the story where we left
off…
Prior to the phone call…
“Achoo,” snorted Donley, causing her drink
to crash to the floor. “My allergies are
acting up again. I haven’t sneezed this hard
since I gave away my dog, Doug. Did
someone let an animal in here?” The eyes
of Walrus, Dangerkitty, and Music casually
shifted to the ground, affixed to the
intricate designs of the lab’s floor tiles. No
one could ever find out that they had stray
dog hairs on their lab coats.
Walrus saw his opportunity to act.
“Donley,” he casually addressed the
scientist who had stolen his chances of
winning the Nobel Prize, “let me get you
another drink.” He nervously staggered to
the mock wet-bar, making sure to turn his
back to his arch-nemesis. After all, he
wouldn’t want to give away the secret
ingredient to his fabulous concoction.
Some people might react negatively toward
him if they knew he kept cyanide on hand…
Donley slowly bent down to pick up
the broken glass on the floor. “Here,
Donley, let me help you with that,”
said Dangerkitty. “Ouch! Ughh. I
cut myself on the glass, now there’s
blood on the floor!”
“Don’t worry,” whispered Donley. “I’ll
clean that up later.”
Sloopy Stretch had been waiting for the
right time to speak to his secret love, and
now seemed to be his last chance of the
night. “Dearest Doodles,” he cooed, “I’ve
waited so long to tell you that…that…” His
lips hesitated and he had to peek at his
notes on a page torn from his diary. “…oh,
Doodles. I love you. I confess! Here, read
this, it explains all of my feelings.”
Donley’s wary fingers took the letter and
began to tear it open.
“Oh Don-l-ey!” A singsong voice of insanity
called out her name. “Here’s that drink you
wanted.” Walrus’ beady eyes concentrated
on her lips as she slowly raised them to her
glass.
“Wait!” interrupted Dr. Sandro Music, it was
the first time that she had spoken in
minutes. “I want to know what the letter
says. Read that first!”
Donley also wanted to know what her secret lover
wished to express, so she quickly scanned the lines of
the dirty and revealing letter. “You creep!” she
shouted, and began to rip the paper to shreds, each
time tearing a piece of Stretch’s heart. “How can I
possibly go on working with you? Get out! Now!” It
was at that moment that Donley decided to seek
comfort in her cocktail. She raised it to her lips in a
quick gesture, obviously directing her anger at Stretch
toward the nearest inanimate object.
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As she began to steadily drink from the cool,
refreshing cocktail, the invader known as cyanide
crept into her system and shut down her electron
transport chain, preventing her from making ATP to
carry out normal functions.
“AHHH,” screamed Music. “What have you done to
her! You’ve killed a brilliant mind! She was about to
help me with my project and look what you did!”
Music’s red eyes glowered down upon the guilty
Walrus, yet his muddled mind could not have cared
less. “Stretch, Dangerkitty, someone…help her…save
her.”
“No! No one will help you in your noble pursuits”
cackled Walrus. “We all wanted her dead. I wanted
her dead so I could win the Nobel Prize. Stretch
wanted her dead so that he can touch her without her
fighting back. Dangerkitty wanted his lab ingredients
back, he didn’t want his precious ideas stolen.”
Walrus became giddy, he was so taken with his clever
plan that he started to dance around, pull out his hair,
and dizzy himself in an effort to celebrate. He didn’t
even notice that everyone else had fled the scene.
Just then, the tip of Walrus’ elbow brushed
against one of Donley’s top-secret test
tubes, as if in slow motion. It crashed to
the ground, exposing the substance
(smallpox) to Walrus. He screamed and
the hysteria mounted as Walrus shot out of
the laboratory, carrying his pot of brew
within the grasp of his sweaty, guilty palms.
It was only a matter of days for the
S.S.S. to discover the guilty parties.
Dr. Red Walrus…
Dr. Red Walrus, on the edge of a
nervous breakdown, easily gave up.
He pleaded guilty because of insanity
and was sent to a home. They let
him bring his stinky brew, which
turned out to be a rare form of
almond juice.
Dr. Sandro Music
Dr. Sandro Music was let off the hook
because she was the one who called
the S.S.S. for help and had no
motivation to kill such a brilliant
scientist.
Dr. Sloopy Stretch
Dr. Sloopy Stretch was not convicted of
murder, but was punished for
stalking Dr. Donley. He has since
acquired a new love.
Dr. Lazarus Dangerkitty
Dr. Lazarus Dangerkitty fled from the
scene and was unable to be located
by the S.S.S. (this is very hard to do
because the S.S.S. is composed of
geniuses). Some speculate that he
ran off with his robo-cats and is now
the leader of the Robo-Cat Cult
(R.C.C.)
…and the S.S.S.
Agents Moore, Eastman, Stork, and
Hansen of the S.S.S. became famed
crime detectives.
In fact, they made so much money that
they decided to retire early and are
now living on remote islands around
the world.
Case Closed