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NATIONAL FOOD AND VETERINARY RISK ASSESSMENT INSTITUTE Gediminas Pridotkas Director 31/01/2011 Vilnius LABORATORIES OF THE SFVS NFVRAI Territorial Branches Laboratories of counties SFVS NATIONAL FOOD AND VETERINARY RISK ASSESSMENT INSTITUTE Lithuanian competent body for risk assessment in the fields of food safety and animal health Mission – to provide scientific information and scientific and technical support in pursuance State policy in the fields of food and feed safety and veterinary and communicate on risks Established in 2008 (originally in 1945) Branches in Kaunas, Panevėžys and Šiauliai 275 employees NATIONAL FOOD AND VETERINARY RISK ASSESSMENT INSTITUTE Accreditation: • ISO/IEC 17025 (DAkkS, Germany) • Federal Centre of Hygiene and • Epidemiology of the Federal Service for the Protection of Consumer Rights and Human Well Being (Rospotrebnadzor, Russian Federation) Federal Agency on Technical Regulating and Metrology (GOST-R, Russian Federation) ILAC logo since 2007 STRUCTURE OF THE NFVRAI THE APPROACH TO RISK ANALYSIS EU – EFSA LT - NFVRAI Risk Assessment EU - EC LT – SFVS Risk Management Hazard identification Hazard characterization Exposure assessment Risk characterization Legal acts Official control Education EFSA NFVRAI Risk EC SFVS Communication Consumers Business operators Veterinary medicines and biocides RISK ASSESSMENT Feed 1. Hazard identification 2. Hazard characterization 3. Exposure assessment 4. Risk characterisation Food Animal health and welfare RISK ASSESSMENT DEPARTMENT SCIENTIFIC AND TECHNICAL ASSISTANCE LEGISLATION CONTROL COMMUNICATION THE APPROACH TO RISK ASSESSMENT Analysis of Laboratory and Veterinary data Analysis of scientific information Research work SCIENTIFIC AND TECHNICAL ASSISSTANCE Scientific cooperation Competence of experts SCIENTIFIC OPINIONS OF RISK ASSESSMENT DEPARTMENT IN 2009 Risk assessment criteria for feed operators Use of bioethanol by-products for feeding of animals Possible risk of influenza A subtype H1N1 virus to human and animal health Risk assessment of infectious laryngotraceheitis in poultry Classification of feed materials Possible negative impact of veterinary medicinal product Domitor in dogs Assessment of eradication plan of the bovine viral diarrhea Preventive vaccination against bluetongue disease Risk assessment of meat preparations Assessment of impact of ammonium to human health Laboratory analysis of imported products of animal origin Risk assessment of high, medium and low risk food establishments Risk assessment of elevated level of boron in natural mineral water Risk assessment of marketing of raw milk Risk assessment of soft ice creams Risk assessment of imported animal origin food from third countries LABORATORY DEPARTMENT NRLs in 36 fields covered by CRLs for food, feed and animal diseases Laboratory tests of biological, chemical and other risk factors in food and feed Diagnosis of animal diseases Epidemiological analysis of animal infectious diseases and zoonoses Validation and coordination of laboratory test standards and methods TARGETS OF INVESTIGATIONS STATE OFFICIAL CONTROL AND MONITORING PROGRAMS • Monitoring of contaminants in food of plant origin • Monitoring plan for certain substances and residues in live animals and animal origin food products • Environment monitoring program • Program for the control of imported foodstuffs • Program for the control of animal infectious diseases • Monitoring program of feed • Plan for investigation of official samples of food and drinking water • Monitoring of Listeria monocytogenes in food DIAGNOSTIC OF ANIMALS INFECTIOUS DISEASES Bacteriology Unit Serology Unit Virology Unit Pathological Anatomy and Histology Unit Molecular Biology and GMO Unit BACTERIOLOGY UNIT Bacteriological tests of clinical and pathological material and feedstuffs Parasitological tests Culture identification Prophylactic analyses Antimicrobial resistance Examination of antimicrobial substances in feedstuffs VIROLOGY UNIT Virological tests of domestic animals, fish and wild animals Cell culturing, VI, VN, FAVN, IF, IFA technique Biosafety Level 3 facilities SEROLOGY UNIT Monitoring programs: • • • • • • • • Bovine, ovine and caprine brucellosis Enzootic bovine leucosis Classical swine fever Swine vesicular disease Foot and mouth disease Aujeszky’s disease Avian influenza Newcastle disease More than 50 infectious diseases can be tested for animals intended for trade, shows and competitions Tests on milk (EBL, BRC) and sera PATHOLOGICAL ANATOMY AND HISTOLOGY UNIT Necropsy or postmortem examination of dead animals, birds Gross pathology and histological analyses of delivered samples Preparation of pathological material for virological, bacteriological and toxicological analyses Testing of transmissible spongiform encephalopathies (TSE) by histological imunohistochemical and rapid test methods Microscopic analyses of components of animal origin in feedstuffs CONTROL OF FOODSTUFFS AND FEEDSTUFFS Chemistry Unit Food Microbiology Unit Sensory Analyses Group Molecular Biology and GMO Unit Radiology Unit CHEMISTRY UNIT Chemical and physical analyses of foodstuffs, their raw materials, drinking and mineral water, beverages, feedstuffs and other Evaluation of composition of products – proteins, fats, hydrocarbons, vitamins, humidity, ash content and other Contaminants – mycotoxins, pesticide residues, polychlorbiphenils, benzo(a)pirene, residues of veterinary medicinal products and feedstuff additives, toxic elements FOOD MICROBIOLOGY UNIT Qualitative and quantitative microbiological, mycological, parasitological analyses of foodstuffs, water, beverages Tests of irradiating foodstuffs Detection of residues of antibacterial substances in raw milk and fresh meat Media and reagents production and quality control SENSORY GROUP 12 assessors were selected and trained in cooperation with Sensory Analysis Laboratory of Technological Institute in Denmark Sensory analyses of foodstuffs, drinking water, pharmaceutical raw materials RADIOLOGY UNIT Radiological tests of foodstuffs and feeds, their raw materials and water Control of imported goods from the third countries Blood biochemical analyses MOLECULAR BIOLOGY AND GMO UNIT Molecular qualitative and quantitative analyses of foodstuffs, feedstuffs, plants and their seeds Molecular microbiological, virological analyses to determine rabies, classical swine fever, avian influenza, bovine viral diarrhoea and other diseases Polymerase chain reaction (PCR), AT-PCR, real time PCR, genotyping and sequencing analyses TERRITORIAL BRANCHES Bacteriology analyses subdivision – bacteriological, parasitological and mycological tests of clinical and pathological material of animals Serology analyses subdivision – infectious diseases in domestic animals and wild fauna (analysis from milk and blood) Microbiology analyses subdivision – quantitative and qualitative microbiological, mycological, parasitological analyses of foodstuffs Chemistry analyses subdivision – chemical and physical analyses of foodstuffs, drinking and mineral water, beverages, feedstuffs; blood biochemical analyses Pathological anatomy and histology subdivision – sections of dead animals, gross pathology and histological analyses of delivered samples Virology analyses subdivision – analyses of rabies of animals LABORATORY INVESTIGATIONS IN 2004-2010 1200000 1023334 944035 1000000 845875 824982 795482 800000 670144 600000 637702 400000 200000 0 2004 2005 2006 2007 2008 2009 2010 NUMBER OF LABORATORY INVESTIGATIONS IN 2010 57% (472592) Commercial tests 43% (352390) Official control programs NUMBER OF LABORATORY INVESTIGATIONS AND NON-COMPLIANT CASES IN 2007-2010 900000 800000 700000 845875 795482 824982 600000 637702 Laboratory investigations 500000 400000 Non-compliant cases 300000 200000 100000 18246 19227 12035 17406 0 2007 2008 2009 2010 NUMBER OF NON-COMPLIANT FOOD AND FEED SAMPLES IN 2010 Indicators Residues of antimicrobial substances Residues of veterinary medicinal products Chloramphenicol Residues of pesticides Heavy metals Salmonella spp. Legionella Listeria monocytogenes Iron (drinking and mineral water) Parasites in food products Dioxins, furanes and PCBs Organoleptic parameters GMO Number of samples 23 11 27 23 28 73 5 38 226 173 2 42 14 UNSAFE FOODSTUFFS AND FEEDINGSTUFFS IN 2009 Product Frozen pike Rice Frozen pork neck meat Food supplements Canned herring fillet Sunflower oil Grapes Nuts butter Apples Waffle Frozen fish fillet Pistachio Frozen poultry meat Canned fish Chicken leg meat Pet food Chocolate sweets Orange Pomegranate Feedingstuffs Country of origin Amount, t Indicator China Pakistan United Kingdom Canada Belarus Ukraine Turkey The Netherlands Poland Turkey Kazachstan Iran Poland Russia Poland USA Turkey Turkey Israel The Netherlands 14 25 5,6 0,64 2,05 59,12 19 1,17 0,1 0,74 16,2 12 10,6 14,1 3,5 19,4 7,2 19,5 1,6 300 Nematodes Mycotoxins Organoleptic parameters >5% GMO Listeria monocytogenes Benzopyrene Metoxychlore Organoleptic parameters Chlorbuphame >5% GMO Trematodes Aflatoxins Salmonella spp. Nematodes Listeria monocytogenes >5% GMO >5% GMO Chlorpyrifose λ-cihalotrine >5% GMO UNSAFE FOODSTUFFS AND FEEDINGSTUFFS IN 2010 Product Country of origin Amount, t Indicator Eggs Lithuania 360 pcs. Salmonella spp. Rice Brazil 0,002 Organoleptic parameters Orange Egypt 59,67 Phenitrotione Orange Egypt 20,28 Daiazinone Sweets Turkey 0,69 >5% GMO Pig muscle Lithuania 9,77 Spectynomycine, lincomycine Frozen fish fillet Russia 18 Nematodes Herring from Baltic Sea Lithuania 0,3 Dioxins Poultry meat Poland 1005 Salmonella spp. Feedingstuffs The Netherlands 2005,5 >5% GMO Golden raisins Iran 2380 Carbendazim and benomyl Sausages "without preservatives" Lithuania 725,61 Preservatives Drinking water Lithuania 360 pcs. Pseudomon Green grapes India 15840 Chlormequat Black tea India 200 Monocrotophos Pomegranate Egypt 100 Methomyl and thiodicarb Pasta with chicken aroma China 1760 Aluminum Hot smoked sausages Lithuania 50 Listeria Monocytogenes B Ar raz ge il nt in Be a lg iu m Cz ec h Eg yp t In In dia do ne sia Ir a Ic n ela nd Sp ain Ita ly Ch in Po a M lan ac ed d on ia Pe ru Ru ss i Tu a rk e Fi y nl a Sl nd ov en Vi ia etn a Uk m ra in e Number of consignments of unsafe imported foodstuffs 2010 12 10 10 9 7 2 1 7 4 3 1 1 1 9 8 8 7 6 4 3 2 2 2 1 1 1 1 0 Number of samples tested for residues in live animals and products of animal origin in 2010 in Lithuania 1179 1200 1000 957 800 594 600 Animals and products of animal origin 49 38 Fish Honey 89 206 Milk Rabbits Pigs 0 99 Poultry 13 Wild animals 14 Farmed wild animals 17 Sheep and goats 200 Horses 198 Eggs 400 Bovine animals Number of samples 1400 Prevalence of residues in live animals and products of animal origin in 2009 – 2010 in Lithuania 0,7 0,5 0,43 0,6 prevalence of residues (%) 0,5 0,3 0,4 0,2 0,3 0,2 0,1 0 2010, animals (N = 2030; n = 6) 2010, animal origin products (N = 1423; n = 3) 2009, animals (N = 1800; n = 9) Animals and animal origin products 2009, animal origin products (N = 1632; n = 7) Number of non-conformities of feed safety (monitoring) and labeling in 2009 – 2010 in Lithuania 4,5 3,2 Number of unconformities (%) 4 3,5 2,12 3 2,5 2 1,5 1 0,5 0 2010 (N = 377; n = 12) 2009 (N = 189; n = 4) Investigation of imported consignments in 2009 – 2010 in Lithuania 20 18,6 Number of positive samples (%) 18 16 14 12 10 10 8 6 4 7 5,6 4,1 2 0 0 0 0 2010 2009 2010 2009 2010 2009 2010 2009 (NF; P; N = (NF; P; N = (PF; P; N = (PF; P; N = (PF; L; N = (PF; L; N = (PF; S; N = (PF; S; N = 160; n = 9) 102; n = 19) 74; n = 3) 70; n = 7) 108; n = 0) 215; n = 15) 119; n = 0) 33; n = 0) Samples NF – non processed fish; PF – processed fish; P – parasites; L – Listeria; S – Salmonella. NUMBER OF POSITIVE CASES OF ANIMAL CONTAGIOUS DISEASES IN 2010 Disease Number of positive cases Rabies Efficiency of oral vaccination of wild animals against rabies Salmonella spp. infections Colibacteriosis Streptococcal infections Staphylococcal infections Anaerobic infections Pseudomonosis Mastitis Enzootic bovine leukosis Leptospirosis Infectious bovine rhinotracheitis (Ab) Bovine viral diarrhoea (Ab) Bovine respiratory syncytial viral infection (Ab) Bovine parainfluenzae 3 (Ab) Mycoplasmosis (Ab) Aujeszky’s disease (wild boars, Ab) Parvoviral infection (Ab) Newcastle disease (Ab) *- Antibodies after vaccination 33 71,1 % (tetracycline marker) / 59,6 % (Ab in blood) 17 131 99 195 34 51 1620 465 299* 333 189 212 182 4007 13 419* 199* Prevalence of rabies in 2009 – 2010 in Lithuania 9 5,6 8 Prevelance (%) 7 2,8 6 5 4 3 2 1 0 2009 (N = 1143; n = 64) 2010 (N = 1166; n = 33) Efficacy of intake of baits with vaccine by wild animals in 2009 – 2010 in Lithuania 78 Efficacy (%) 76 74,4 74 71,1 72 70 68 66 2009 (N = 2459; n = 1830) 2010 (N = 4448; n = 3161) Seroconversion of wild animals vaccinated against rabies in 2009 – 2010 in Lithuania 70 59,6 60 49,7 Effectiveness (%) 50 40 30 20 10 0 2009 (N = 290; n = 144) 2010 (N = 1164; n = 694) Effectiveness of vaccination against rabies of pets in 2008 – 2009 in Lithuania 105 Effectiveness (%) 100 95 89,8 98,2 99,3 Cats (2008; N = 109; n = 107) Cats (2009; N = 143; n = 142) 92,5 90 85 80 Dogs (2008; N =548; n =492) Dogs (2009; N = 624; n = 577) Pets Prevalence of Trichinella in domestic pigs (DP) and wild boars (WB) in 2009 – 2010 in Lithuania 7 6,2 6 Prevalence (%) 5 4,6 3,9 4 3 3 2 1 0 2009 (DP; N = 230; n = 9) 2010 (DP; N = 433; n = 27) 2009 (WB; N = 3181; n = 95) Animals 2010 (WB; N = 2149; n = 98) Investigation of prevalence of Salmonella in poultry flocks in 2009 – 2010 in Lithuania 3,6 3,3 3,2 2,8 prevalence, % 2,4 2 1,6 1,2 1 0,7 0,8 0,5 0,3 0,4 0,2 0 0 0 Breding (N 527; At the hatchery n 0) (N 479; n 1) Epheneral (N 296; n 2) Pullets (N 285; n 3) Lying hens (N 333; n 11) category of poultry Eggs (N 173; n 0) Broilers in flocks Broiler carcasses (N 707; n 2) (N 420; n 2) Number of bovine animals tested for BSE in 2009 – 2010 in Lithuania Number of tested bovine animals 90000 80000 84112 77168 70000 60000 50000 2009 40000 2010 30000 20000 10000 1936 1577 243 0 Slaughtered Emergency slaughtered 206 With clinical signs Risk category 2168 2004 Dead Thank you for your attention!