Transcript Elterninformation / Prof. Dr. med. Zacharias Zachariou

O
Effect of air pollution exposure during
pregnancy on the steroid profile of newborns
Rüedi S*, Proietti Elena*, Gorlanova O, Dick B, Flück C, Röösli M, Latzin P,
M. Frey B, Frey U
Introduction
• Prenatal air pollution exposure has adverse
effects on the infant’s respiratory system.
• One pathway involved might be via oxidative
stress, possibly leading to a modified pattern of
steroid production.
Aim
We examined a possible association of air
pollution exposure during pregnancy and steroid
metabolites in the newborn’s urine.
Air Pollution
pregnancy
Steroids profile
at one month
Methods – BILD cohort
Prenatal
recruitment
Age 1
month
Urin
collection
Methods – exposure and outcome
Air Pollution
pregnancy
 PM10 from background
monitoring station
 NO2
• background monitoring
station and
• hybrid time-space
regression model
Steroids profile
at one month
• Spot urine
• mass spectrometry/
gas chromatography
Methods - statistic
Steroids profile
at one month
Air Pollution
pregnancy
Adjusted for:
season
distance to road
urban area
smoking exposure
socio-economic status
Sex, gestational age, weight
delivery mode
Methods - steroids
11- β HSD2 (NAD+)
Cortisol F
Cortisone E
11- β HSD1 (NADH)
CYP3A
5β-reductase
5β-DHF
6β-OHF
5β-reductase
5α-DHF
3αHSD
3αHSD
THF
α-THF
DHE
THE
20aHSD/20βHSD
β-cortol
α-cortol
α-cortolone
β-cortolone
Results – PM10 (aRR per 10mg/m
3
PM10 increase)
All
1.46 (1.06/2.02)
N=129
Cortisol/Cortisone
1.04 (0.81/1.34)
Branche F
1.42 (0.96/2.1)
Branche E
1.45 (1.06/1.99)
All metabolites
1.6 (1.14/2.28)
Branche F metabolites
1.62 (1.04/2.54)
Branche E metabolites
1.60 (1.14/2.23)
Results – NO2 background (aRR per 10mg/m
All
No association
3
NO2 increase)
N=120
Cortisol/Cortisone
No association
4.10 (1.6/10.6)
6βOH cortisol
4.9 (1.6/14.9)*
Branche F
No association
Branche E
No association
All metabolites
No association
*distance to road (50m)
aRR 0.98 (0.97/0.99)
*Pathologic CTG
aRR 1.6 (1/2.5)
Results – NO2 model (aRR per 10mg/m
All
No association
Cortisol/Cortisone
No association
6βOH cortisol/F
1.14 (0.9/1.5)
6βOH cortisol
1.3 (0.9/1.7)
Branche F
No association
Branche E
No association
All metabolites
No association
3
NO2 increase)
N=120
Conclusions
Air pollution exposure during pregnancy is
associated with alterations of the steroid profiles in
newborns’ urine.
PM10 exposure during pregnancy is associated with
an overall increase of stress steroids production.
Conclusions
background NO2 is associated with increased 6bOHcortisol 6bOH-F/F ratio, which may suggest
induction of the cytochrome enzymes.
If air pollution modelling was purely based on home
location of mothers without considering maternal
mobility, the risk ratio significantly decreased
Even at low environmental doses of air pollution
we found steroids response as know trigger such
as perinatal stress
[email protected]
Thank you
OHF
RR
CI
CI
p-value
No2 background
4.96
1.64
14.94
0.01
sex
1.00
0.73
1.37
1.00
Gest age
0.89
0.79
0.99
0.04
sectio
0.99
0.66
1.48
0.96
smoke
0.89
0.60
1.32
0.55
education mid
0.75
0.48
1.16
0.19
education upper
0.72
0.47
1.12
0.15
studywgt
1.00
1.00
1.00
0.02
Road dist 50m
0.98
0.97
1.00
0.01
urban
0.90
0.65
1.25
0.54
season
1.56
0.92
2.65
0.10
Methods - DAG
Smoking
Maternal
SES
education
Pregnancy behaviour
psychological stress
Urban/rural
home
Air pollution
Season
sex
sectio
Gestational
age +
weight
Steroids
PM10 effect on overall steroids production –
different adjustments
PM 10 models
2.5
2.0
RR
1.5
1.0
0.5
0.0
crude
season
perinatal
adjustments
traffic
perinatal+traffic
10
0
15
5
20
25
Frequency
15
20
Air Pollution exposure of the BILD cohort
(region of Bern – Switzerland)
14
16
NO2 background
18
20
40
30
20
30
NO2 model
10
20
0
10
Frequency
0
40
5
50
10
12
10
15
20
PM10 background
25
European Environment Agency – NO2 annual averages 2005