Transcript Biotechnology: Presant Status, future directions

Biotechnology:
Presant Status & biosafety concerns
Department of Agriculture
H. M.J.Bandara
Plant Quarantine Officer
Sri Lanka
Department of Agriculture
Mandatory Crops:
Cereals, legumes, condiments, fruit crops,
root & tuber crops, oil seed crops vegetables,
floricultural plants
Activities:
Research & development, Seed certification &
plant protection, Planting material production
Distribution & Plant quarantine, Germplasm
management, Botanic gardens, Agriculture
extension, Education & training,
Finance & administration
Biotechnology
Tissue Culture
Molecular techniques (Based on nuclear
acid & proteins)
Biotechnology is defined in many ways.
Tissue culture is considered one of the
techniques. It’s associated directly with genetic
transformation.
HORTICULTURAL CROP
RESEARCH AND DEVELOPMENT
INSTITUTE- Tissue Culture Program
Banana Tissue Culture
Papaya tissue culture
HORTICULTURE CROP RESEARCH
AND DVELOPMENT INSTITUTE
Virus indexing (serological)
Banana
Papaya
Citrus
Microbiology
Food Research Unit
Fermentation Food analysis
PLANT VIRUS INDEXING CENTER
Serology and PCR Based Virus
Indexing- Banana Virus
Pineapple
RT PCR for Detection of RNA Virus
SITHA ELIYA POTATO RESEARCH
CENTER:
Tissue Culture Program
Potato
Straw berry
Pineapple
RARDC, Bandarawela
Tissue Culture Program
Potato
Anthurium
Gerbera
Strawberry
Madonna lily
Virus indexing of potato by ELISA
RARDC, Agunakolapelessa
Banana
•Thubakarwial
RICE RESEARCH AND DEVELOPMENT
INSTITUTE:Rice Biotechnology
Anther Culture
Exploitation of Somaclonal Variation
Wide Hybridization
NATIONAL BOTANIC GARDENSTissue Culture Program
Propergation of Endemic & rare Plants
Floriculture Plants
Mutation induction
PLANT GENETIC RESOURCE CENTER:
Tissue Culture Program
Banana
Citrus spp
• In vitro pineapple potato Conservation
• Papaya tissue Culture
PLANT GENETIC RESOURCE
CENTER: Tissue Culture Program
Anther Culture in rice Haploid Culture
a good system for transformation
PGR/RRDI- Rice research
PCR based DNA markers for breeding/gene
pyramiding
•Gall midge resistance
•Thrips resistance
•Bactria leaf blight resistance
•BPH resistance
Genetic Characterization by DNA markers
Mango -
Mangifera zeylanica
Magifera indica
Maize(PGRC/MI)
Finger printing of hybrids
Pathogens - Ralstonia, BLB
Onion Characterization & breeding
Introgression of genes from wild
relatives
Wild tomato X tomato
Wild okra X okra
Future Work
SSR markers for rice
AFLP for fingerprinting of germplasm &
Varieties
Northern blot techniques for
Analysis of HSP regular gene
Expression
Isolating the fragment for Coat
protection gene papaya Ring spot virus
Application of Tools in Biosafety
Regular Measures
Tissue Culture Safe handling of materials Restricted
multiplication
Serological methods Testing &detection of foreign
Genes bases in proteins
Serological method should not be the sole method.
Genes expression (transgenic)is spatially &
temporally controlled
PCR/Blot methods Based on DNA More sensitive
method.Detect only presence or foreign DNA
National Plant Quarantine Service
Requirements
With the existing infrastructure & function
and provided with additional strength
(Trained staff, Detection equipment, Risk
assessments Studuo) NPQS could
undertake the regulatory activity
Institutional Biosafety Committee
Liaison with the national committees
Identification of institutes:transformation
work,GMO detection /testing,regulation & risk
assessment
Preparation of guidelines on biosafety
regulations,risk assessments & management
Maintain constant access to relevant data
base or registers both local & foreign
Monitoring & evaluation of programs
Building awareness
Requirements for Implementation
of the Biosafety Procedures
Policy level support
Financial,administrative & legislative support
Biosafety clearing house mechanism
Monitoring, evaluation & inspection services
Training of decision makers,&technical level
scientists & technicians
Facility enhancement
Public awareness, education & promotion of
public participation including stake holders.
GENETIC Transformation of
Agricultural Crops
Policy level decisions & if necessary, changes
Identification of priority problems/crops
Identification of Institutes
Facility development