Transcript The secondary antibody
Choose a category. You will be given a question. You must give the correct answer.
Outbreaks Immune System Vocabulary
Enzyme-Linked Immunosorbant Serological Assay
What are the 2 types of ELISA tests?
Antigen Detection ELISA & Antibody Detection ELISA
What is significant about the secondary antibody created for the ELISA test?
Is created to specifically bind the primary antibody
It is conjugated with an enzyme
What reaction occurs in an ELISA, causing a visible color change?
The enzyme oxidizes the substrate?
Why are antibodies used to identify the presence of a particular antigen?
Antibodies are only created in response to a specific antigen and will only bind that one antigen
What is a medical intervention?
Any measure that improves health or alters the course of a disease.
List 3 interventions that could be documented for Sue Smith
List 3 major medical intervention categories
Diagnostics Surgery Medications rehabilitation Genetics Immunology Medical devices
Which medical intervention category does an ELISA fall into?
List 3 interventions that could be used for a broken leg.
Xray Pain meds Cast Crutches/wheel chair surgery
What is the liquid used to dilute a substance called?
How is a tube dilution calculated?
Amount of sample _________________ Total volume in tube
Calculate the tube dilution for the following tube:
Calculate the final dilution for tube #4
If tube #1 has a concentration of 100 ug/ml, what is the concentration in tube #3?
A rise in disease occurrence in an area
List 4 steps taken by scientists during an outbreak
What is the best medical intervention to determine patient zero?
Quantitative concentration data from an ELISA.
How do scientists determine the pathogen responsible for the outbreak?
Pathogen DNA isolation and sequencing
1. Determine patient zero 2. Track the path of infection
Which immune system structure produces antibodies?
How many antigens will an antibody bind?
An identifying protein marker on the surface of cells
What does the presence of the specific antibody for HIV in a patient’s system indicate?
That patient has encountered the HIV virus in their body
Why is antigen concentration alone sometimes not enough to determine patient zero?
A patient’s immune system may have already reduced the amount of pathogen present
What is a specific causative agent of disease?
The amount of a specified substance in a unit amount of another substance
How does a visible color change in an antigen detection ELISA prove the presence of the antigen?
The enzyme connected to the secondary antibody oxidizes the substrate to produce a color change. The secondary antibody is not present unless the primary antibody is present. The primary antibody is not present unless the antigen is present. Therefore, the presence of the secondary antibody proves the presence of the antigen.