Transcript ChemWell ELISA Analysis

Introducing automated
TM
ELISA - ChemWell
Geoffrey Anderson
Unitech Scientific LLC
Lakewood, CA
Visit us:
www.unitechscientific.com
TM
ChemWell
Wine Autoanalyzer
Manufactured by Awareness Technology
Unitech Scientific
- Exclusive Distributor
First analyzer in the world to combine biochemistry
and microplate EIA into one instrument.
ChemWell uses microwells for all reactions, chemistry and EIA.
A ChemWell Tour
2 syringe pumps
Optical system
Wash head
Windows 95, 98 or XP
DI water
Wash
and
rinse
bottles
Reagents
Samples
reactions
ChemWell
Recommended workload
Designed for labs doing:
100 - 1000 tests per day
or
400 EIA per 8 hours
ChemWell Features
& Benefits
• Easy to Use
• Continuous Sample Loading
• “Stat” sample priority
• Low Operating Cost
• Intuitive, Open Software
• Proven, Reliable Technology
Calibration Options
Cut-off (Semi Qualitative)
 Multi-point
Polynomial fit
 Cubic Spline fit . . . and more
Store Calibration Curves
Wine Controls: Chart Levy Jennings
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ChemWell runs ELISA as
well as Enzymatic reagents
Unitech offers Histamine ELISA kits
Botrytis and Aspergillus ELISA kits
are in development
So let’s take a quick review of the steps and
principals of ELISA reagents.
Immuno Complex: an Antigen
with its matching Antibody
Ag
Ab
An antigen and its antibody fit together
like 3 dimensional puzzle pieces.
Diagram of a simple ELISA
with a positive sample (sample has Ag in it)
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Mix the sample and diluent together in a
microwell that is pre-coated with specific Ab
Incubate for x minutes until the Ag in the sample
binds to the Ab on the well walls.
Start with an
Ab-coated well
Ab
Add the sample
with some Ag in it
Ab
Ag
Ag
Ab-Ag
Ab-Ag
Ab
Ab
Incubate until
complexed
Ab
Ab
AbAb
Conjugate is a specific Ab
bound to an Enzyme.
Wash out the
remaining sample
Ab-Ag
Add a
conjugate
Ab-Ag
Ab
Ab-Ag
Ab-E
Ab-E
Ab
Ab-E
Ab-Ag
Incubate until
complexed
Ab-Ag-Ab-E
Ab
Ab-E
Ab-Ag-Ab-E
The conjugate has a second antibody to the same antigen, allowing you to make
an antigen sandwich (with antibody bread). It also leaves an enzyme hanging off.
Washing . . . removes the unbound
conjugate, without removing bound antibodies.
Wash out the
remaining conjugate
Ab-Ag-Ab-E
Ab
Ab-Ag-Ab-E
Add a
substrate
S
Ab-Ag-Ab-E
Ab
S
S
Substrate + Enzyme
produces color
Ab-Ag-Ab-E-S
Ab
Ab-Ag-Ab-E
S
Ab-Ag-Ab-E-S
The more Enzyme available, the more color will develop.
Many ELISA’s use a stopping solution to end the color generating step before
reading.
Reliable Results:
Enzymatic and ELISA
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Precise pipetting
Washes probe inside and out
Liquid sensing
Temperature controlled
Easy to Maintain

Proven Reliability, over 500 sold
Self Diagnosis
Phone/email support by Unitech
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Modular Design
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Economical to Use
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Dramatically reduces LABOR
 Laboratory
 Administrative
Warranty
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1-year parts & labor Warranty
Extended Warranty available

6-Month Preventative Maintenance

Free replacement of warranteed parts
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Other repairs if required

Loaner instrument within 72 hours
Easy to own
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Price start at $27,500
Reagent rack cooling option
Add $2000
ChemWell Distributed by Unitech Scientific