Transcript PowerPoint-presentatie - q

In search for whitefly attractants
Willem Jan de Kogel, Chamila Darshanee Lunuwila,
Rob van Tol, Antje de Bruin & Gerrie Wiegers
Bemisia tabaci
 Occur in Europe
 Whiteflies from non-European origin may contain qviruses
 Therefore introduction of non-European B. Tabaci not
allowed
 WP4: improve detection of whiteflies
 WP7: detect q-virus in whiteflies
Aim
 Find whitefly attractants for improved
detection/monitoring/control
 Find tomato cultivars that are more attractive then
others
 Check if whitefly – tomato plant interaction affects
attractivity
 Analyse chemical compounds involved in attraction
 Develop attractants in practical tools
Y-tube Olfactometer
Air
Charcoal
Filter
Charcoal
Filter
Sucking pump
Y-Tube
each arm 5cm
Flow meter
Wind speed
Temperature
= 10cm/S
= 21-25 0C
Olfactometer: preference for Santa Clara over Money maker
(small difference)
Olfactometer:
Male whiteflies prefer infested tomato “Santa Clara” plants
over uninfested plants
Infested
Uninfested
Santa Clara
Money Maker
Wind Tunnel Experiments
Windtunnel: whitefly infestation increases attractiveness of
Santa Clara.
Windtunnel: whitefly infestation more attractive than mechanical
damage
Windtunnel: Infested Santa Clara preferred over infested
Moneymaker
Greenhouse trial: result confirmed
Insect release platform
Greenhouse trial: result confirmed
Insect release platform
Conclusion:
 Tomato cultivars differ in attractivity to Bemisia tabaci
 Previous infestation increases attractivity strongly
 Results y-tube, windtunnel greenhouse consistent
 Attractivity (at least partly) due to plant odour
Follow-up:
 Follow-up: option to continue topic as PhD-project:
● Publish results
● Identify volatile compounds by headspace collection of
infested vs control Santa Clara plants followed by GC-MS
● Test potentially interesting compounds with bio-assays
● Test attractants in combination with yellow sticky traps in
greenhouse