Transcript MYOGRAPHIE ISOMETRIQUE

MYOGRAPHIE ISOMETRIQUE
AORTE
n=souris (x4 anneaux)
0.60
lox/lox (n=6)
cre (pkd) (n=10)
0.50
lox/lox cre (n=5)
N=19
Tension (mN/mm)
lox/+ cre (n=6)
0.40
N=17
N=17
0.30
N=22
0.20
0.10
0.00
0
50
100
-0.10
K+ concentration (mM)
150
4,00
lox/lox (n=12)
3,00
2,50
lox/+ (n=1)
cre (pkd) (n=11)
2,00
lox/lox cre (n=9)
1,50
lox/+ cre (n=12)
1,00
0,50
0,00
-9,0
-8,5
-8,0
-7,5
-7,0
-6,5
-6,0
-5,5
-5,0
5-HT (Log)
1,80
lox/lox (n=12)
1,60
Delta tension / Delta tension max
Delta tension (mN/mm)
3,50
lox/+ (n=1)
1,40
cre (pkd) (n=11)
1,20
1,00
lox/lox cre (n=9)
lox/+ cre (n=12)
0,80
0,60
0,40
0,20
0,00
-9,0
-8,5
-8,0
-7,5
-7,0
5-HT (Log)
-6,5
-6,0
-5,5
-5,0
8,00
lox/+(n=1)
7,00
cre (pkd) (n=10)
6,00
lox/lox cre (n=8)
5,00
lox/+cre (n=12)
4,00
3,00
2,00
1,00
0,00
-11,0
-10,0
-9,0
-8,0
-7,0
-6,0
U46619 (Log)
Control
8,00
7,00
lox/lox cre
6,00
Delta tension
Delta tension
lox/lox (n=10)
lox/+cre
5,00
4,00
3,00
2,00
1,00
0,00
-11,0
-10,0
-9,0
-8,0
U46619 (Log)
-7,0
-6,0
Delta tension (mN/mm)
0.45
0.40
lox/lox (n=20)
0.35
cre(pkd) (n=22)
0.30
lox/lox cre (n=17)
0.25
lox/+ cre (n=17)
0.20
0.15
0.10
0.05
0.00
-9.0
-8.0
-7.0
Phenylephrin (Log)
-6.0
-5.0
0 Ca2+ egta Mg2+
PSS + thapsigargin 500 nM
+ thapsigargin 500nM + Phe 1µM
+ caffeine 10 mM
+ Phe 1µM
V-dpt Ca channels
Internal stores
Store operated channels
2.00
1.80
lox/lox (n=4)
1.60
cre (n=7)
Force (mN)
1.40
1.20
lox/lox cre (n=7)
1.00
lox/+ cre (n=3)
0.80
0.60
0.40
0.20
0.00
+Phe1µM (peak)
+Phe1µM (1 min)
zero pss thapsi
+ Phe 1µM (1 min)
+ Phe 1µM (2 min)
PSS + thapsigargin
MYOGRAPHIE ISOMETRIQUE
ARTERE MESENTERIQUE
n=anneaux