Mass Spectrometry Analysis of GoldView

ACRIDINE ORANGE

ACRIDINE ORANGE 34 (1.215) Cm (32:34-21:22) 100 266.4

531.3

19-Sep-200614:07:32

Scan ES+ 4.17e6

Acridine Orange from Aldrich 252.0

532.2

Acridine orange dimer 268.1

0 143.4

161.9178.5

206.1

233.9

238.9

GOLD VIEW 24 (0.865) Cm (20:28-(13:14+47:48)) 100 280.0

281.0

310.0

325.8

357.0

399.1

385.1

400.1

413.4

443.1

488.3

517.1

533.3

504.1

534.2

567.1

569.1

594.1

531.2

629.0

650.1

664.1

666.3

694.1

Scan ES+ 3.29e6

Acridine orange dimer GoldView 532.2

252.0

268.1

567.1

0 142.9

154.9

120 140 170.9

160 180 209.0

221.8

238.9

200 220 240 260 280.0

280 306.1310.2

300 320 338.2

364.2

340 360 379.2

405.0

424.1

440.1 444.9

490.2

504.2

517.1

380 400 420 440 460 480 500 520 533.3

534.2

540 560 569.1

580 603.3

600 629.3

647.1

664.2

620 640 660 685.8 693.2

680 m/z 700

• • • • (1) (2) (3) (4)

Conclusion

The effective ingredient in GoldView is acridine orange (extremely cheap from Aldrich or Sigma at ~100g/$140!) Acridine orange is a known gel stain since 1970s (“Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange”.

Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. Acridine orange is a highly toxic and known mutagen!

Selected list of papers on acridine orange toxicity:

Web, RB. Mutat Res. 1984 Jul;137(1):1-6.

Wugmeister, M. Yale J Biol Med. 1983 Jan-Feb;56(1):9-13.

Martin, SE. Mutat Res. 1981 Jun;82(1):41-6.

Ashad, R. Letters in Applied Microbiology 2006, 42:94.