Orion QuikRead

®

Portable CRP Test Kit

Supplies

• QuikRead ® 101 instrument • Magnetic calibrator card • Blood • Capillary tubes with plungers (20 uL) • Cuvettes • Waterproof marker • Buffer with 1-mL dispenser • CRP reagent caps

Intended Use

• QuikRead ® CRP is a quantitative test of CRP in whole blood, serum or plasma • CRP is an acute phase protein associated with bacterial infection, inflammation or tissue destruction

Test Principle

• Immunoturbidimetric test based on microparticles coated with anti-human CRP F(ab) 2 • CRP in sample reacts with the microparticles and increases solution turbidity, proportional to the CRP concentration

Steps

• Turn on QuikRead ® 101 instrument • Lot-specific calibration curve, coded onto a magnetic card, is loaded into instrument • Buffer is dispensed into cuvettes • Blood (or serum or plasma) is added to buffer and mixed • RBCs are lysed and blank is read

Steps (cont.)

• Pre-calibrated reagents are dispensed from caps • Capped cuvettes are mixed by vigorous shaking • Sample is read in instrument • Result is recorded before removing cuvette • Instrument performs self-check before next test

Measurement Range

• 5 – 180 mg/L

Analytical Precautions

• Make sure that the magnetic card and reagents have matching lot numbers • Do not expose magnetic card to strong magnetic fields • Do not touch the optical part (lower half) of the cuvettes • Clean the dispenser regularly with deionized water

Analytical Precautions (cont.)

• Do not expose the reagent caps to moisture • Check for air bubbles in buffer dispenser tube and cuvettes • Do not splash liquid into the measuring well of the QuikRead ® 101 • Results must be recorded before removing cuvette from instrument or else the result is lost

Hematocrit Correction

• Calibration assumes a hematocrit of 40% • Correction formula for different hematocrits 60 (plasma volume) 100 – actual hematocrit X CRP test result For example, 60 (plasma volume) 100 – 33 X 5 mg/L = 4.5 mg/L Alternatively, use table of correction factors provided with kit