Transcript Investigation of a novel functionalized peptide as a biomaterial

In vitro evaluation of biomaterials for
bone tissue engineering
Maria Chatzinikolaidou
Biomaterials
Dept. Materials Science and Technology
University of Crete, P.O. Box 2208 GR71303 Heraklio, Crete, Greece
[email protected]
Research activities
 In vitro biocompatibility study of biomaterials,
including cell adhesion, viability, proliferation and
morphology of mammalian cell lines and stem cells
 Investigation of the osteoinductive potential of
implantable biomaterials  bone tissue repair
 Development of immobilization techniques for
proteins on biomaterials surfaces
 Adsorption and desorption studies of proteins on
biomaterials surfaces
Cell adhesion shown by SEM
Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material, structured by twophoton polymerization
After 4 hours
After 3 days
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki
Cell adhesion and proliferation
shown by SEM
Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material, structured by twophoton polymerization
After 6 days
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki
Cell adhesion shown by SEM
Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material – meshes structured by
two-photon polymerization
After 4 hours
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki
Cell formations during adhesion
Early event
Late event
Extracellular factors and integrin-derived signaling trigger activate actin
polymerization at the leading-edge membrane (as an early event) and the
formation of focal adhesions (as a later event)
Biomaterials: The Intersection of Biology and Materials ScienceJohnna S. Temenoff, Antonios G. Mikos
Cell morphology of MC3T3-E1
Visualization of vinculin and actin cytoskeleton by means of
confocal microscopy
Cell source: mouse pre-osteoblasts MC3T3-E1
Biomaterial: organic-inorganic composite material
Overlay of double staining with ΤRΙTC-conjugated falloifin and
FITC-conjugated anti-vinculin
magnification 20x
Cell morphology
Visualization of vinculin and actin cytoskeleton by means of
confocal microscopy
Cell source: mouse pre-osteoblasts MC3T3-E1
Biomaterial: organic-inorganic composite material
Overlay of double staining with ΤRΙTC-conjugated falloifin and
FITC-conjugated anti-vinculin
magnification 40x
Acknowledgements
Collaborations
Prof. Maria Vamvakaki, UoC
Dr. Maria Farsari, Prof. Costas Fotakis, IESL-FORTH
Prof. Eleni Papadaki, UoC
A reservoir for progenitor cells
http://anatquest.nlm.nih.gov
Organic-inorganic composite material
Methacryloxypropyl trimethoxysilane (MAPTMS),
(2-dimethylamino)ethyl methacrylate (DMAEMA) and
methacrylic acid (MAA) as the polymerizable monomers
Zirconium n-propoxide (Zr(OPr)4, and the trimethoxysilane
groups of MAPTMS serve as the inorganic network forming
moieties