Transcript 獲得原廠提供免費FuGENE ® HD Transfection Reagent 客戶須知

獲得原廠提供免費FuGENE® HD Transfection Reagent
客戶須知詳細內容
A.
由原廠免費獲得回饋禮後，請客戶提供實驗結果
B.
檔案規格:
相
關
規
定
圖片檔(以下擇其一)：

細胞株/primary細胞之bright field 及fluorescent field 照片

免疫染色照片

Flow cytometry 結果照片 (需配合negative control)
*Pixels: 寬-1000, 高-667; dpi:200
C.
註：

頁數
1.
為標準與基本要求
2.
為格式說明，紅字部份與方框為說明
3.
為空白格式，請客戶依格式要求寄回此頁

檔案可至www.genelabs.com.tw 之FuGENE HD® Transfection Reagent產品介紹網
頁下載使用。

檔案完成後回傳至 [email protected]，並註明單位、PI及提供者姓名。
勁因科技有限公司 www.genelabs.com.tw
訂貨專線：台北02-26557678,台中04-22633813,台南06-2094380. 全省技術支援電話：0800-231914
台南免付費電話：0800-094380. FAX:02-26557572 E-mail：[email protected]
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格
式
說
明
U-937, Blood(Cell,Origin)
(fluorescent field)
(bright field)
B
A
細胞名稱
U937 cells were transfected using the FuGENE® HD Transfection Reagent
and 0.5 µg of a plasmid encoding the EGFP. 24 hours post transfection, the cells were
analyzed by light (A) and fluorescence microscopy (B).
DNA量、DNA名稱與觀察記錄時間若有更動請自行修改。
I. Transfection conditions
Size of
Cell Line
Culture Transfected
Reagent:
DNA Ratio
Amount of DNA
Assay by
Transfection
Viability
Efficiency
Used (per well)
□ IFA
U937
24 well plate
6:1
0.25µg
□ FACS
□ 客戶自評
□Others_____
II. Cell information
勁因科技有限公司 www.genelabs.com.tw
訂貨專線：台北02-26557678,台中04-22633813,台南06-2094380. 全省技術支援電話：0800-231914
台南免付費電話：0800-094380. FAX:02-26557572 E-mail：[email protected]
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(fluorescent field)
(bright field)
B
A
______cells were transfected using the FuGENE® HD Transfection Reagent
and ____ µg of a plasmid encoding the EGFP. ____ hours post transfection, the cells were
analyzed by light (A) and fluorescence microscopy (B).
I. Transfection conditions
Cell Line
Size of
Reagent:
Amount of DNA
Culture Transfected
DNA Ratio
Used (per well)
Assay by
Transfection
Viability (%)
Efficiency (%)
□ IFA
□ FACS
□ 客戶自評
□Others_____
II. Cell information
Cell type/Description
Characteristics/Species
Tissue Origin
Media
Morphology
Double time
Subculturing
Culture condition
勁因科技有限公司 www.genelabs.com.tw
訂貨專線：台北02-26557678,台中04-22633813,台南06-2094380. 全省技術支援電話：0800-231914
台南免付費電話：0800-094380. FAX:02-26557572 E-mail：[email protected]
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