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5 days,
6-10 March 2017,
international teachers
Advanced level
Early registration: November 30th 2016
Multicolor
FLOW CYTOMETRY
Basics in Cellular Immunology
FCM Techniques
Protocol design & settings
Data analysis & interpretation
Research and Diagnosis
Good practice, Accreditation
Organizers : Claude LAMBERT (F)
Ulrich SACK (D)
Enrique O’CONNOR (E)
for
Researchers
Clinical Biologists
PhD students
Technologists
R&D pharma
Information:
[email protected]
http://cytima2016.emse.fr/winter_school.php
https://www.chromocyte.com/
In vitro differentiation of
T cell phenotypes and
subsets Ulrich SACK (Leipzig) human dendritic cells
General review on
applications
9:30-10:30
Dendritic cells exercises
Practicals on T Cells
17:30-18:30 analyses
PMA Activation test
technique exercises
Phagocytosis and
Bactericidy
T cell functions
Andrea COSSARIZZA
Practicals on B Cells
15:00-16:00 analysis (lmd)
Immunopathology and
Sepsis
Fabienne
VENET (Lyon)
Immunoparalysis,
Monitoring Granulocyte
reactivity Guillaume
MONNERET (Lyon)
Assays for the assessment of PMA Activation test
16:30 - 17:30 T cell activation and function technique
detection of rare events
of interest
Andrea COSSARIZZA
Practicals on B Cells
14:00:00-15:00
analysis (lmd)
B-cells Development and
differentiation
Innate Lymphoid Cells
Myelopoiesis (Thomas
11:00 - 12:00 Ulrich SALZER (Freiburg) Frederic VELY (Marseille) MATTHES Geneva)
Phagocytosis Bactericidy
Innate Lymphoid Cells
Fouad SEGHROUCHNI
12:00 - 13:00 Ulrich SALZER (Freiburg) Frederic VELY (Marseille) (Rabat Morocco)
TP tests PMA Ionomycin
preparation
8th March
Wednesday
Flow cytometric analysis of
human peripheral blood
dendritic cells
9:00 Welcome
7th March
Tuesday
8:30-9:30
6th March
Monday
Immunology
Saint-Etienne France
Multiple functional assay
Bar coding
Fatima
L'FAQIHI (Toulouse)
T cell functional testing
Iyadh DOUAGI (Stokholm)
10th March
Friday
Conclusions, feedback
Signaling,
phosphorylation
Primary
immunodeficiencies
Ulrich SACK (Leipzig)
T cell Activation test
Antonio COSMA (Paris)
Cytoplasmic Cytokines
Myeloid lineage
clustering using Mass
cytometry antonio
COSMA (Paris)
Image flow exercises
Imune synapse : Image
flow cytometry
Livine DUBAN
Basophil Activation Test
Degranulation tests Paul
ROUZAIRE (Clermont
Ferrand)
Cytoplasmic Cytokines
Lymphopoiese T
Ulrich SACK (Leipzig)
9th March
Primary Thursday
immunodeficiencies in
clinic JL Stephan (SaintEtienne)
6-10 March 2017
2nd Winter School of Cell Analysis