Transcript Ionization characteristics of glycosides in direct analysis in real time

Electronic Supplementary Material (ESI) for New Journal of Chemistry.
This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2016
Ionization characteristics of glycosides in direct analysis in real
time quadrupole-time of flight mass spectrometry
Hongmei Yang,*,a,d Ge Gao,c Yihan Wang,a Jinrong Liu,b Zongjun Lid, Rui Su,a,b
Bing Wang,b Wenhui Lian,a Xinhua Guob and Shuying Liu*,a,d
aChangchun
University of Chinese Medicine, Changchun 130117, China.
E-mail: [email protected], [email protected]
bDepartment
of Chemistry, Jilin University, Changchun 130012, China.
cDepartment
of pathology, China-Japan Union Hospital, Jilin University, Changchun
130033, China.
dChangchun
Institute of Applied Chemistry, Chinese Academy of Sciences, 5625
Renmin Street, Changchun 130022, China.
Supplemental Information
Figure Legends
Fig. S1 Plots of influence of temperature on signal intensity. (a) 10 g/mL 2nitrophenyl -D-galactopyranoside, (b) 10 g/mL hyperoside, and (c) 10
g/mL 2'-deoxyadenosine in positive ion mode.
Fig. S2 He-DART mass spectra of 10 g/mL hyperoside at different
fragmentor voltages. (a) fragmentor voltage: 10 V; (b) fragmentor voltage: 50
V; (c) fragmentor voltage: 100 V; (d) fragmentor voltage: 200 V; (e)
fragmentor voltage: 300 V; (f) fragmentor voltage: 400 V.
Fig. S1
1.1E+07
(a)
1.0E+07
9.0E+06
8.0E+06
7.0E+06
250
Absolute Abundance
1.0E+07
300
350
400
300
350
400
300
350
450
500
550
(b)
8.0E+06
6.0E+06
4.0E+06
2.0E+06
0.0E+00
250
1.6E+07
450
500
550
(c)
1.4E+07
1.2E+07
1.0E+07
250
400
450
Temperature
500
550
Fig. S2
2500000
3000000
(a)
Y0 3
303.05
Y30
303.05
(b)
Absolute Abundance
Absolute Abundance
2000000
1500000
[M+H]+
465.11
1000000
500000
627.16
4000000
400
500
600
m/z
700
[M+H]+
465.11
1000000
M+B03
627.16
[2M+H] +
929.21
M+B0 3
300
2000000
800
Y30
303.05
0
900
Y30
303.05
400
600 m/z
[2M+H]+
929.21
800
(c)
(d)
2000000
[M+H]+
465.11
1000000
M+B03
627.16
8000000
4000000
3
Y0
400
600
m/z
2000000
[M+H]
465.11
+
4000000
2000000
[2M+H]+
929.21
3
M+B0
627.16
400
8000000
(e)
600
m/z
800
[2M+H]+
929.21
M+B03
627.16
800
303.05
6000000
[M+H]+
465.11
[2M+H]+
929.21
0
0
Absolute Abundance
Absolute Abundance
3000000
Absolute Abundance
Absolute Abundance
6000000
400
Y30
303.05
600 m/z
800
(f)
+
6000000
[M+H]
465.11
4000000
2000000
0
M+B03
627.16
400
600 m/z
[2M+H]+
929.21
800