Transcript Figure S3

1
1
0.8
0.8
OD600
B 1.2
OD600
A1.2
0.6
Figure S3
0.6
0.4
0.4
0.2
0.2
0
0
0
15
30
45
60
75
Time (minutes)
90
105
120
0
15
30
45
60
75
90
105
120
Time (minutes)
prz1OE + zym
EV + zym
prz1OE + zym
EV + zym
∆pvg1 prz1OE + zym
∆pvg1 EV + zym
∆pvg5 prz1OE + zym
∆pvg5 EV + zym
Figure S3 Cell wall degradation assays. (A) The zymolyase-resistant phenotype from
overexpression of prz1+ was abrogated by loss of pvg1+ (p=6.8e-3). (B) The ∆pvg5 strain does
not suppress the zymolyase-resistant phenotype from overexpression of prz1+. The samples
were adjusted to matching cell densities and transferred to test tubes in the presence and
absence of 25 U/mL Zymolyase 100T. The samples were shaken at 37°C and OD600 readings
were taken every 15 minutes to assess the degree of cell wall degradation. The zymolyase
experiments were repeated in triplicate and error bars represent the standard error. The Pvalues were determined with ANOVA followed by a two-tailed student t-test after two hours of
zymolyase treatment.