Transcript 下載/瀏覽Download

Biosensors and Bioelectronics 41 (2013) 129–136
On-chip dual detection of cancer biomarkers directly in serum based
on self-assembled magnetic bead patterns and quantum dots
Xu Yu a,b, He-Shun Xia c, Zuo-Dong Sun a, Yi Lin a,b, Kun Wang c, Jing Yu c, Hao Tang a,c, DaiWen Pang a,b, Zhi-Ling Zhang a,b,
a Key
Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and
Molecular Sciences, and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China
bWuhan Institute of Biotechnology, Wuhan 430075, PR China
c Hubei Cancer Hospital, Wuhan 430079, PR China
Advisor : Cheng-Hsin Chuang
Advisee : Kai-Chieh Chang
Department of Mechanical Engineering
& Institute of Nanotechnology,
Southern Taiwan University of Science and Technology, Tainan, TAIWAN
Date ﹕2013/11/4
Outline
Paper Survey
• Experimental
• Result and discussion
2
Experimental
Fig. 1. Schematic diagrams of an integrated MFCM-Chip and sample loading
process. (a) Scheme of Ab-SPMBs loading process. (b) The cross-sectional view of
the integrated MFCM-Chip. (c) Scheme of the sample and reagent loading
process. (d) Imaging of the nickel pattern arrays in eight parallel channels. (e)
Schematic diagrams of the principle of multiplexed detection simultaneously in a
MFCM-Chip. The scale bar is 500 mm.
3
Experimental
Fig. S4. (A) (B) Optical and fluorescence images of
SPMBs. (C) (D) Optical and fluorescence images
of the complexes which were the products of the
immunoreaction of the anti-AFP-SPMBs and antimouse IgG-FITC. The scale bars are 20 μm.
Fig. S5. Optical and fluorescence images of immune
complexes. 1.0 μg/mL CEA was added into two 1.5
mL eppendorf tube with the anti-CEA-SPMBs and
incubated for 30 min. (A) (B) Without biotin-antiCEA adding into the immunoreaction. (C) (D) With
biotin-anti-CEA (10.0 μg/mL) adding into the
immunoreaction. SA-QDs (1 μL, 1 × 10-6 mol/L)
were added into two tubes and incubated for 15 min.
The scale bars are 20 μm.
4
Experimental
5
Fig. S9. Simulation of the absolute value of the
multiplication of magnetic flux density and the magnetic
Fig. S8. A MFCM-Chip was used to detection of 200 ng/mL field gradient ((B⋅∇) B). The scale bar is 50 μm.
CEA for 16 times.
6
Results and discussion
Fig. 3. Multiplexed detection in a MFCM-Chip. (A) Nickel pattern arrays
under eight parallel branch fluid channels. (B) Different captured Ab-SPMBs
in the branch channels. (C) Fluorescence image of detection of multiple target
Ags. From channel 1 to channel 8 to detect AFP, none, rabbit IgG, CEA, rabbit
IgG, AFP, none and CEA, respectively. (D) Fluorescence image after several
minutes’ excitement by blue light. The scale bars are 200 mm.
7
8
Fig. 5. (a) Fluorescence images of detection of different concentrations of CEA. (b) Calibration curves of simultaneous
detection of CEA and AFP from 10.0 ng/mL to 400.0 ng/mL at the exposure time of 400 ms. (c) Calibration curves of
detection of CEA and AFP from 200.0 ng/mL to 2000.0 ng/mL with the exposure time of 200 ms.
(d) Normalized calibration curves of detection of CEA and AFP from 10.0 ng/mL to 2000.0 ng/mL. (e) The linear
relationship between fluorescence intensity and the concentration of AFP and CEA in the range from 10.0 ng/mL to 800.0
ng/mL (R2 ¼0.982 and 0.998, respectively). The scale bar is 50 mm.
9
Results and discussion
Fig. 6. Simultaneous detection of AFP and CEA in clinical serum samples.
The values of background fluorescence intensity were not deducted.
10
Thank you for your attention
11