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Innovate Food Science and Emerging Technologies 12 (2011) 617-622
A microwire sensor for rapid detection of
Escherichia coli K-12 in fresh produce
Sungkyun Kim ,Lin Lu, Jae-Hyun Chung ,Kyonghoon Lee ,Yong Li ,Soojin Jun
Paper Survey
Advisor : Cheng-Hsin Chuang
Advisee :Wan-Ting Su
Department of Mechanical Engineering
& Institute of Nanotechnology,
Southern Taiwan University, Tainan, TAIWAN
Date ﹕2012/04/06
1
Outline
• Paper Survey
 Sensor Design
 Results and discussion
• Experimental Result
• Future work
Sensor Design
Fig .1.
(a) Microwire sensing unit
(b) Power supply and measurement
equipments
Results and Discussion
Fig2.Regression curve for enumeration
of E.coli K-12cells
Fig3.Stepwise produres for detection of E.coli K-12
DEP force and electroosmotic flow
Capillary
Simulation Result
Fig 4. Sequential simulation of electric field strength
Experiment Results
Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire.
(a)Without and (b) with AC field ﹒
Experiment Results
Fig. 6. Optimization of the working frequency
for detection of E.coli K-12 at 106 CFU/ml
Fig .7. Fluorescence intensity of E.coli K-12 in
PBS buffer when applied with 20VPP and 3MHz
Experiment Results
Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution:
(a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations
(b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface
Outline
• Paper Survey
 Sensor Design
 Results and discussion
• Experimental Result
• Future work
Experimental Method
Experimental Results
Applied Time ： 0 min
Applied Time ： 2 min
Flow Rate ： 0.1 ml/hr
Al2O3 Concentration：2 wt%
BSA Concentration：1 μM
Applied Time ： 16 min
Applied Frequency：10 kHz
Apply Voltage：10 Vpp
Applied Time ： 30 min
Outline
• Paper Survey
 Sensor Design
 Results and discussion
• Experimental Result
• Future work
Future work
• Paper Survey
• Experiment by Different Parameter
• Experimental Sample Using Bladder
Cancer Cell
• SEM Training
Thank you for your
attention!!