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CONDENSATION OF FLUORESCENT
NANOPARTICLES USING A DEP CHIP WITH A
DOT – ELECTRODE ARRAY
STUDENT’S NAME : TRAN HONG CHUONG
陳紅章
CONTENT
1
INTRODUCTION
2
DEFINITION OF DEP
(DIELECTROPHORESIS)
The ability of an uncharged material to move when subjected to an electric
field
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DEFINITION OF
MICROCHIPS
Microfluidics deals with the behavior, precise control
and manipulation of fluids that are geometrically
constrained to a small, typically sub-millimeter, scale
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DEFINITION OF FLUORESCENT
Fluorescence is the emission of light by a substance that has
absorbed light or other electromagnetic radiation. It is a form
of luminescence
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SOME METHODS HAVE BEEN
IMPLEMENTED BEFORE THIS PAPER
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PURPOSE OF THIS PAPER
DEP manipulations was considered low cost ; intrinsic electrical
control ; and compability with microfluids .
This paper develops a micro fluidics chip for programmable
condensation of fluorescent – labeled nano particles based on DEP to
enhance the strength of fluorescent density in a dot electrode array.
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DEP THEORY AND NUMERICAL SIMULATION
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DEP EQUATION AND EXPLAIN
For a time-averaged dielectrophoretic force acting on a spherical particle
immersed in a medium and exposed to a spatially non-uniform electric field.
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FDEP  2. . m .R3p .Re .[K ()].Ems
m
Is the electrical permittivity of the
surrounding medium .
Rp
Is the radius of the particle
E  E  E  E
2
ms
2
x
2
y
2
z
Re . K ()  0  FDEP  0
Re . K ()  0  FDEP  0
Re . K ()  0  FDEP  0
Gradient of the square of the applied
electric field magnitude
Particle moved particle possessing a
high intensity electric field.
Particle moved particle possessing a low
intensity electric field.
The suspend particles will not be
affected by the DEP force
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STRUCTURE OF DEP CHIP &
SIMULATION RESULT
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EFFECT OF BROWNIAN FORCE ON
PARTICLE
FB   (12 RP K BT /  )1/ 2

Is a Gaussan random vector

Is a dynamic vicosity of fluid
RP
Is the boltzman’s contrast
T
Is the temperature and T =dt is the time step
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EFFECT OF BROWNIAN FORCE ON
PARTICLE
2
FDEP  2. . m .R3p .Re .[K ()].Ems
FB
RP1/2 ; FDEP
RP3 ; FDEP  FB
FB   (12 RP K BT /  )1/ 2
This meant that the DEP force constructed in our chip was able to sufficiently
manipulate 20 nm particles and overcome the Brownian motion effects.
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MATERIAL AND METHOD
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SAMPLE PREPARATION
In order to quantify the condensation of the NPs using DEP
force, nanoparticles were labeled by fluorescent protein
and observed by fluorescent optical microscopy.
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SAMPLE PREPARATION
In this experiment, we use Low-cost commercial aluminium oxide nano
particles (Al2O3-NPs)
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EXPERIMENT METHOD
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EXPERIMENT METHOD
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EXPERIMENTAL RESULT AND DISCUSSION
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EXPERIMENT RESULT AND
DISCUSSION
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MANY THANKS FOR YOUR ATTENTION!
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