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新陳代謝儀
June , 2010
Britton Chance (born July 24, 1913) is Eldridge Reeves Johnson University
Professor Emeritus of Biochemistry and Biophysics, at the University of
Pennsylvania. He received a Ph.D. degree in Physical Chemistry 1940 at
the University of Pennsylvania. In 1952 he received his D.Sc. from
His research interests focus on the use of light
to characterize the properties of various tissues and
breast tumors. He joined the United States National Academy of
Cambridge.
Sciences in 1952. Chance received the National Medal of Science in 1974.
He was also previously elected a Foreign Member of the Royal Swedish
Academy of Sciences, Medical Sciences, in 1968, as well as a Foreign
Member of the Royal Society (London) in 1981.
何謂新陳代謝儀?
• 以光學、非侵入方式檢視口腔黏膜細胞粒
線體REDOX 比值(NADH/FAD)的儀器。
氣虛症與細胞能量代謝
• 1996年中國醫藥大學院**碩士論文以鈉鉀
泵 的活性與氣虛症相對照，得到明顯的統
計相關。
• 當時作者體內40%~60%的能量是供泵活動
所需，故本項研究以鈉鉀泵作為能量代謝
的評估。
• REDOX 比值為西醫標準的
Supporting Literature
• Coghlan L. et. al, Optimal fluorescence
excitation wavelengths for detection of
squamous intra-epithelial neoplasia: results from
an animal model, Opt Express,. 2000 Dec.,
4;7(12):436-46.
Abstract
Using the hamster cheek pouch carcinogenesis model, we explore which
fluorescence excitation wavelengths are useful for the detection of
neoplasia. 42 hamsters were treated with DMBA to induce carcinogenesis,
and 20 control animals were treated only with mineral oil. Fluorescence
excitation emission matrices were measured from the cheek pouches of the
hamsters weekly. Results showed increased fluorescence near 350-370 nm
and 410 nm excitation and decreased fluorescence near 450-470 nm
excitation with neoplasia. The
optimal diagnostic excitation
wavelengths identified using this model - 350-370 nm
excitation and 400-450 nm excitation - are similar to those
identified for detection of human oral cavity neoplasia.
The Construction
• The electronic engineering design consists
of 450 nm and 370 nm LEDs flashed
alternatively for 10msec. Attached thereto
at a distance of 3 mm but protected by
gelatin filters, respectively at 520 nm and
450 nm, are two Si diode detectors
followed by current to voltage conversion.
The sheep, under appropriate animal subject protocol,
was anesthetized with breathing isofluorane [to avoid a
change of mitochondrial steady state as with Nembutal]
and anesthesia was ensured by standard tests. From
minutes 0 to 10 the device was moved in the cavity to
find a stable location. At minute 14 the inspired gas
was changed to N2 an the redos value in creased from
0.76 to 0.94 with very good signal to noise ratio.
Possible applications
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Biomarkers of oxidative stress,
Biomarkers in Mental Disorders,
Development of pediatric Medical Devices,
Biomarkers of neurodegenerative Diseases,
Oral pre-malignancies
Type 2 diabetes- optimal control,
Obstructive Sleep apnea,
Body burden of emerging contaminants
Telehealth tools for high risk youth
TCM: Qi