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Focusing of biological cells utilizing negative
dielectrophoretic force generated by insulating
structures
Chun-Ping Jen *, Ching-Te Huang, Cheng-Hsin Weng
Department of Mechanical Engineering, National Chung Cheng
University, Chia Yi, Taiwan, ROC
Advisee : Jyong-Sian Lai
Department of Mechanical Engineering
& Institute of Nanotechnology,
Southern Taiwan University, Tainan, TAIWAN
Date ﹕2011/11/11
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Outline
1. Introduction
2. Chip fabrication
3. Numerical Simulations
4. Experimental results
5. Conclusions
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1. Introduction
The main
purpose of the
present study is
to design an
insulator-based
dielectrophoreti
c microdevice
with effective
focusing of
biological cells.
The cells are introduced into the microchannel and preconfined
hydrodynamically by the funnel-shaped insulating structures
close to the inlet.
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2. Chip fabrication
Schematic diagram of the microchip for particle
focusing using insulator-based dielectrophoresis.
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3. Numerical Simulations
Operational concept illustrating focusing by a
dielectrophoretic force acting on the cells.
Transient simulation of tracks of negative dielectrophoretic
cells under varying electric field strengths and inlet velocity.
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4. Experimental results
Table 1
Measured widths of cells distributed at the outlet under
varying electric field strengths and inlet velocity.
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5. Conclusions
• The numerical simulations indicated that an increase in the
strength of the applied electric field significantly enhanced
the performance of focusing. Furthermore, decreasing the
inlet velocity increased the efficiency of focusing because
the higher velocity resulted in greater lateral expansion.
• Experimental results indicated that the performance of
focusing increased with the strength of the applied electric
field and with a decrease in inlet velocity. The
experimental results as exhibited were in accordance with
the trend predicted by numerical simulations.
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Thanks for your attention
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