Transcript Power Point Day 2

Aim: How can we measure area
on ImageJ?
Day 2
Why use area?
• Useful in colony
counts
• Area of growth
• Zone of inhibition
• Can measure multiple
areas simultaneously
How to measure area (single shape)
• Set Scale
• Analyze
• Decide which tool to
• Measure
use- rectangle, ellipse, • Record value for area
polygon (best choice!)
How to measure area (color adjustment)
• This is only for Black and White
photos! (We will do color tomorrow)
• Set scale
• Rectangle tool over scale
– Right click; clear
• Image
– Type
– 8-bit
• Process
– Noise
– Despeckle (2-3x)
How to measure area (color adjustment)
• Process
– Binary
– Make binary
• Make sure entire shape is
one color (despeckle if not)
• Analyze
– Analyze particles
– Show
• Outlines
– Check “Display Results”
Compare original to outline
Assignment
• Junior High Researchers
• Calculate area using polygon AND Color
Adjustment for Image B and Image D
• Senior High Researchers
• Calculate area using polygon AND color
Adjustment for Image C and E