Transcript Power Point Day 1

Aim: What is ImageJ and Why do
we use it?
Day 1
Brief Background
• Released in 1997 by Wayne
Rasband
• Developed at the National
Institutes of Health
• Runs on Windows, Mac, and
Linux
• Macros enable
personalization and allows
for focus on specific tasks
Background
• FREE!! Runs on Java with
no license required
– Predecessors such as
Image SXM and NIH Image
were “closed source”
…requires fee/license
• More than 1700 users on
the mailing list
– Countless others use for
independent research
Why do we need to learn about it?
• Increase technical
computer skills
• More accurate than
hand calculations or
guess-timations
• Allows you to
manipulate data
Ok, so what is it?
• Long answer: a public domain, Javabased image processing program
• Short answer: pixel analysis
How to use ImageJ (The basics)
• Go to My Website (Mrs. Farley) through the
Elmont web page
• Right-click to open ImageJ in a new tab
– If you are working at home, download the
program so you can avoid step 1.
• If you have images saved, use those.
– If not, right click sample files and save them to
desktop or another folder
How to set Scales
• File
– Open
– Select image
• Go back to ImageJ
toolbar
• Straight Line
Selections
• Click to start line
on scale
• Click to stop line
on scale
How to set scales
• Analyze
• Set Scale
• Change known
distance to units
on scale in picture
• Change unit of
length to units
provided
(km,m,mm,um,
etc.)
• Click global
• Click OK
How to measure length
• Use straight line tool
• Draw line on selected
area
• Analyze
• Measure
• Scroll to the right of
data table
• Find length; record
How to measure length with a nonstraight line
Segmented Line
• Right click the straight line
tool
• Select segmented line
• Click repeatedly to match
line shape to original image
• Analyze is the same
Free-Hand
• Right click the straight line
tool
• Select freehand
• Click and drag
• Analyze is the same
• **NOT RECOMMENDED**
Assignment
• Junior High Researchers
– Analyze images B, C, and D for distance of one side
and total perimeter
• Senior High Researchers
– Analyze images A-E for “height”, “Width” and total
perimeter
• Email data to your teachers today (Mr.
Spinnato or Mrs. Flannory)